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헤어리베치 수확 시기에 따른 종자 수량, 발아율 및 경실종자 비율 변화 양상
구자환(Ja Hwan Ku),김민태(Min Tae Kim),손범영(Beom Young Son),이진석(Jin Seok Lee),김정태(Jung Tae Kim),황종진(Jong Jin Hwang),백성범(Seong Bum Baek),문중경(Jung Kyung Moon),권영업(Young Up Kwon) 한국초지조사료학회 2012 한국초지조사료학회지 Vol.32 No.2
This experiment was conducted to investigate an optimum harvest time for hairy vetch (HV) seed production. The seeds were harvested at 5 times, 35, 42, 48, 54, and 58 days after flowering (DAF) in 2009 and 2010. In 2011, 3 times of harvest, 39, 49, and 53 DAF were done at Suwon, middle part of Korea. HV plants were harvested and threshed by manually at each time. Seed yield, yield components, germination rate, hard seed rate, and viviparous germination rate were investigated. The highest seed yield represented 54 DAF in 2009, 42 DAF in 2010, and 49 DAF in 2011. When the triticale, HV support plant, comes to early lodging, the time of highest yield was delayed. 1,000-seed weight linearly increased from 35 DAF to 54 DAF. The later harvested seed showed a trend of increasing germination and hard seed ratio. However, viviparous germination had occurred because of in rainy season. In conclusion, it was presumed that an optimum harvest time for safe seed production in HV should be during 42 DAF ~ 54 DAF before the rainy season considering seed yield, 1,000-seed weight and germination. 본 시험은 친환경농업 및 조사료 용도로 재배가 증가하고 있는 헤어리베치를 대상으로 국내 헤어리베치 종자 채종 시 개화 후 경과 일시에 따른 채종 수량 추이를 조사하여 안전 다수확 채종 기간을 구명하기 위하여 경기도 수원시에 위치한 농촌진흥청 국립식량과학원 시험포장에서 헤어리베치 품종 '청풍보라'를 이용하여 2009년부터 2011년까지 3개년에 걸쳐 시험하였다. 2009년도는 개화 후 35일 (6월 11일), 42일 (6월 18일), 48일 (6월 24일), 54일 (6월 30일), 58일 (7월 4일) 등 5회, 2010년도는 개화 후 35일 (6월 21일), 42일 (6월 28일), 48일 (7월 4일), 54일 (7월 10일), 58일 (7월 14일) 등 5회, 그리고 2011년에는 개화 후 39일 (6월 21일), 49일 (7월 1일), 53일 (7월 5일) 등 3회 수확하여 종자 수량과 발아율을 조사한 결과 종자 수량은 개화 후 경과 일수와 비례하여 증가하다가 개화 후 42일 (2010년도), 49일 (2011년도), 54일 (2009년도)에 정점을 나타낸 이후 도복으로 인한 꼬투리 소실로 수량이 감소하였다. 수확 시기가 늦어질수록 1,000립 중과 발아율이 증가하였으나 장마기 이후에 수확 된 종자는 수발아 종자 발생이 증가하였다. 이상의 본 시험 결과에 의하면 헤어리베치 종자 안전 다수확을 위한 수확 시기는 개화 후 42일에서 54일 사이에 장마 전 기간으로 판단되었다.
겔스캐너를 이용한 변성아크릴아마이드 겔의 형광 DNA 검출
구자환(Ja-Hwan Ku),정지웅(Ji-Ung Jeong),조영찬(Young-Chan Cho) 한국작물학회 2005 한국작물학회지 Vol.50 No.S
형광 염료와 레이저 겔스캐너 장비를 이용하여 변성아크릴 아마이드 겔에서 전기 영동된 DNA를 신속하고 간편한 방법으로 기존의 은염색법과 비슷한 감도로 검출하고자 하였다. 변성아크랄아마이드 겔을 형광 염료인 SYBR Green (Molecular Probes)이나 Vistra Green (Amersham Bioscience) 0.01 X 희석액 (pH 8)으로 염색한 후 480㎚ 레이져, 520 ㎚ filter 옵션으로 스캔하여 DNA를 검출하였으며, 검출감도는 기존의 은염색법과 비슷하면서 염색 단계를 한 단계로 줄일 수 있었다. The denature polyacrylamide gel stain silver nitrate is used for routine nucleic acid detection. More sensitive stains, such as Vistra Green, SYBR Green are available to address a broad range of DNA applications requiring lower detection limits in polyacrylamide gel formats. Gel Scanners, laser-scanning instruments, provide sensitive fluorescence detection of DNA gel stains. We established one step fluorescent impregnation enhanced sensitivity with simple, rapid and low cost. We have applied this fluorescent staining procedure for the routine analysis of DNA profiles generated by SSR amplification.
구자환(Ja-Hwan Ku),김민태(Min-Tae Kim),한옥규(Ouk-kyu Han),권영업(Young-Up Kwon),김충국(Chung-Guk Kim),전원태(Weon-Tai Jeon),조현숙(Hyeoun-Suk Cho),강위금(Ui-Gum Kang),오인석(In-Seok Oh),이정태(Jeong-Tae Lee),양창휴(Chang-Hyu Yang) 한국육종학회 2015 한국육종학회지 Vol.47 No.4
In order to develop a hairy vetch (Vicia villosa Roth) cultivar which is suitable for cropping after the rice harvest in middle and northern regions because the cultivar can stably pass the winter season and produce much green manure, the National Institute of Crop Science developed the ‘Ontobi’, an elite cultivar through the method of line breeding after having selected good plants with superior growth rate which were derived from the germplasm ‘Ch4 (introduced from China)’. As main characteristics, ‘Ontobi’ has vivid green leaves during the blooming period and its stem hair’s density is high, and its blooming period is May 15 on average nationwide, and its average blooming period is 5 days later than that of ‘Cheongpoongbora’ the check variety. The Ontobi’s overwintering rate is 87% on average, showing 2% higher than that of the check variety, and its dry matter yield is 5.44 ton/ha on average, showing 17% more than that of the check variety, and its nitrogen content is 172kg/ha on average, showing 13% more than that of the check variety. ‘Ontobi’ can be cultivated in every region nationwide having its average lowest temperature in January is over -15℃.
Ku, Ja-Min,Kim, Dae-Kyun,Ryu, Taek-Hee,Jung, Eun-Hwan,Lansac, Yves,Jang, Yun-Hee Korean Chemical Society 2012 Bulletin of the Korean Chemical Society Vol.33 No.3
Push-pull-type copolymers - low-band-gap copolymers of electron-rich fused-ring units (such as cyclopentadithiophene; CPDT) and electron-deficient units (such as benzothiadiazole; BT) - are promising donor materials for organic solar cells. Following a design principles proposed in our previous study, we investigate the electronic structure of a series of new CPDTBT derivatives with various electron-withdrawing groups using the time-dependent density functional theory and predict their power conversion efficiency from a newlydeveloped protocol using the Scharber diagram. Significantly improved efficiencies are expected for derivatives with carbonyl [C=O], carbonothioyl [C=S], dicyano [$C(CN)_2$] and dicyanomethylene [C=$C(CN)_2$] groups, but these polymers with no long alkyl side chain attached to them are likely to be insoluble in most organic solvents and inapplicable to low-cost solution processes. We thus devise several approaches to attach alkyl side chains to these polymers while keeping their high efficiencies.