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H-Y 에 대한 단일클론 항체의 생산 및 그 이용에 관한 연구 1 . H-Y 에 대한 단일클론항체의 생산
심호섭(H . S . Shim),김재화(J . H . Kim),이병철(B . C . Lee),김종배(J . B . Kim),박홍양(H . Y . Park),정길생(K . S . Chung) 한국축산학회 1988 한국축산학회지 Vol.30 No.7
Testis supernatant, a source of H-Y, obtained from BALB/c mice was used to immunize females of same strain. B lymphocytes of mouse producing antibodies to H-Y were fused with SP2/0-Ag 14 myeloma cells and distributed to 384 wells of 96-well microtiter plates. Eighty hybridoma colonies were formed, resulting in 20.8 percent of fusion efficiency. Three strong positive wells from hybridoma colonies were selected for cloning by ELISA and two of them were also found to be positive by indirect immunofluorescence test. Twelve wells of ELISA-positive were selected after cloning and 2D45D4 clones from them were confirmed to produce monoclonal antibodies to H-Y by indirect immunofluorescence test.
송해범,심금섭 대구대학교 농업과학연구소 1987 農業科學硏究論文集 Vol.1 No.-
Ejaculated and/or epididymal spermatozoa of goats were preincubated for 4-6 h in the isolated genital tracts from hamsters, gilts and goats, or for 3-8 h in m-KRB solution. After preincubation, they inseminated the goat ovulated eggs collected shortly after ovulation and/or the follicular oocytes with intact and without cumulus cells cultured for 25 h in m-KRB solution, and the oocytes with dispersed cumulus cells which were not cultured. The results obtained were as follows. (1) None of the ovulated eggs were fertilized after insemination with ejaculated and epididymal spermatozoa preincubated for 4-5 h in the isolated genital tracts from hamsters, gilts and goats. But 50 and 38% of the ovulated eggs were fertilized with epididymal sermatozoa preincubated for 5 and 6 h in m-KRB solution, respectively. (2) None of the follicular oocytes were fertilized after insemination with ejaculated spermatozoa preincubated for 4-6 h in the uteri isolated from hamsters and goats, and with epididymal spermatozoa preincubated for 4-6 h in the isolated hamster uterus. But 38% of the follicular oocytes were fertilized with epididymal spermatozoa preincubated for 5 h in the isolated goat uterus. (3) After further culture with spermatozoa for 18-24 h, 50-89, 89 and 14-33% of the oocytes with intact, with dispersed and without cumulus cells had matured to the second metaphase, respectively. (4) When epididymal spermatozoa were preincubated for 5 h at the concentration of 4.2x10^(8)/ml and 6 h at the concentration of 3.5x10^(8)/ml in m-KRB solution, 36 and 33% of the oocytes with intact cumulus cells were fertilized. (5) The results suggested that epididymal spermatozoa can be capacitated in m-KRB solution, and that the follicular oocytes matured in culture could be used for the study of fertilization in vitro instead of the ovulated eggs.
Jang, K.J.,Kwon, G.S.,Jeong, J.W.,Kim, C.H.,Yoon, H.M.,Kim, G.Y.,Shim, J.H.,Moon, S.K.,Kim, W.J.,Choi, Y.H. Society for Bioscience and Bioengineering, Japan ; 2015 Journal of bioscience and bioengineering Vol.119 No.3
Cordycepin is an adenosine analog originally extracted from Cordyceps militaris that possesses many pharmacological effects including immune activation and antioxidant and antitumor effects. However, the underlying relationship between apoptosis and telomerase activity in response to cordycepin exposure has not been investigated. In this study, we found that cordycepin-induced apoptosis of human leukemia cells (H937 and THP-1 cells) was associated with inactivation of telomerase and downregulation of human telomerase reverse transcriptase (hTERT) as well as the transcription factors c-Myc and Sp1, which are required for basal transcription from the hTERT gene promoter. Cordycepin also attenuated the activation of phosphoinositide-3-kinase (PI3K)/Akt signaling, thereby reducing phosphorylation and nuclear translocation of hTERT. We further showed that the PI3K inhibitor LY29004 significantly decreased telomerase activity in cordycepin-treated cells and increased cordycepin-induced cell death. These findings demonstrate that cordycepin is cytotoxic to human leukemia cells and suppresses telomerase activity through transcriptional and post-translational suppression of hTERT by inactivating the PI3K/Akt signaling pathway.
南基桓,沈在燮 충북대학교 엽연초연구소 1974 煙草硏究 Vol.2 No.-
This experiment was carried out in order to observe the effects three elements of fertilizer(N.,P.,K.,) on the infection of tobacco brown spot disease. The plots were designed by split plot method with 8 treatment (A: check, B: 50% added applied N., P. and K., C: 50% subatracted applied three elements., D: 50% added applied N., E: 50% added applied P., F: 50% added applied P., G: no applied P., G: an applied P: and H: no applied P.). Check is N 14kg P2O5 21kg and K3O 28kg per 10 are respectively. Y.S.A was employed for sample variety and transplanted on 15 May. The results obtained as follow; 1) There were no differences among the plots (treatments) during the early growing stages. 2) The plot of 1½N, no K. and 1½N.P.K. have shown larger number of lesions than the check respectively. 3) The plot of 1/2 N.P.K.(C), 1½K(F), and no P.(G) have shown almost no differences in the rate of disease occurence as compared with check (A). In conclusion, excess application of N. and defficiency of K. seemed to be related with tobacco brown spot disease infection.
High-Performance Blue InGaN Laser Diodes With Single-Quantum-Well Active Layers
Ryu, H.Y.,Haleem, K.H.,Lee, S.N.,Jang, T.,Son, J.K.,Paek, H.S.,Sung, Y.J.,Kim, H.K.,Kim, K.S.,O.H. Nam,Park, Y.J.,Shim, J.I. IEEE 2007 Photonics Technology Letters Vol.19 No.21
<P>The authors report on the high-performance blue laser diodes (LDs) with an emission wavelength of ~448 nm employing InGaN single-quantum-well (QW) active layers. At 100-mW continuous-wave (CW) output power, operation current and voltage are, respectively, 150 mA and 5.3 V, corresponding to the wall plug efficiency of >12%, a record value for the single-mode InGaN LDs with blue wavelengths. The single QW blue LD showed normal temperature dependence of light output-current curves with the characteristic temperature of 170 K. In addition, we demonstrate a high level of catastrophic optical damage of >300 mW and long device lifetime under CW operation condition at room temperature.</P>
Park, J.K.,Lee, D.H.,Cho, C.H.,Yuk, S.S.,To, E.O.,Kwon, J.H.,Noh, J.Y.,Kim, B.Y.,Choi, S.W.,Shim, B.S.,Song, M.K.,Lee, J.B.,Park, S.Y.,Choi, I.S.,Song, C.S. Elsevier Scientific Pub. Co 2014 Veterinary microbiology Vol.169 No.3
Avian influenza virus (AIV) subtype H9N2 has been evolving rapidly and vaccine escape variants have been reported to cause circulation of infections and economic losses. In the present study, we developed and evaluated ectodomain of the AIV matrix 2 (M2e) protein as a supplementing antigen for oil-based inactivated H9N2 vaccine to increase resistance against vaccine escape variants. AIV H9N2 M2e antigen was expressed in Escherichia coli and supplemented to inactivated H9N2 oil emulsion vaccine. Specific pathogen-free chickens received a single injection of inactivated H9N2 oil emulsion vaccines with or without M2e supplementation. At three weeks post vaccination, hemagglutination inhibition tests and enzyme-linked immunosorbent assays were performed to determine serological immune responses. Challenge study using a vaccine escape H9N2 variant was performed to evaluate the efficacy of M2e supplementation. M2e antigen supplemented in oil emulsion vaccine was highly immunogenic, and a single M2e-supplemented vaccination reduced challenge virus replication and shedding more effectively than non-supplemented vaccination.
PSNR estimation scheme using coefficient distribution of frequency domain in H.264 decoder
Shim, S.-Y.,Moon, J.-H.,Han, J.-K. IET 2008 Electronics letters Vol.44 No.2
<P>Peak signal-to-noise ratio (PSNR) is commonly used as a measure for the quality of the reconstructed picture where the original picture is used as reference data. Since the original picture is not available in the video decoder, the receiver cannot evaluate the quality of the reconstructed image from the viewpoint of PSNR. Proposed is an efficient method to estimate PSNR using the statistical distribution of the integer transform coefficient in an H.264 decoder.</P>