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배승섭,나정걸,이성목,강성균,이현숙,이정현,김태완,Bae, Seung Seob,Na, Jeong Geol,Lee, Sung-Mok,Kang, Sung Gyun,Lee, Hyun Sook,Lee, Jung-Hyun,Kim, Tae Wan The Korean Society for Microbiology and Biotechnol 2015 한국미생물·생명공학회지 Vol.43 No.3
초고온성 고세균 Thermococcus onnurineus NA1은 개미산, 일산화탄소, 또는 전분 등을 이용해서 수소를 생산하는 것으로 알려져 있다. 본 연구에서는 T. onnurineus NA1의 고정화 세포를 이용한 수소생산을 고찰하였다. 고정화 실험결과, T. onnurineus NA1은 표면에 아민기가 코팅된 규조토 담체에 정전기적 인력에 의해 효과적으로 고정화되었고, 1 g의 담체에 고정화 될 수 있는 최대 세포의 양은 71.7 mg-dcw로 확인되었다. 고정화 세포를 이용한 세 번의 반복회분식 배양을 통해 개미산으로부터 수소생산 특성을 고찰하였고, 그 결과 배양이 반복됨에 따라 고정화 세포 농도의 증가에 기인하여 초기수소생산속도가 2.3 에서 4.0 mmol l<sup>−1</sup> h<sup>−1</sup>로 상당량 증가됨이 관찰되었다. 따라서, T. onnurineus NA1의 고정화세포 시스템은 수소생산을 위한 좋은 대안이 될 수 있을 것으로 사료된다. 본 연구는 초고온성 고세균의 고정화세포를 수소생산에 적용한 첫 번째 사례이다. Previously we reported that the hyperthermophilic archaeon, Thermococcus onnurineus NA1 is capable of producing hydrogen (H<sub>2</sub>) from formate, CO or starch. In this study, we describe the immobilization of T. onnurineus NA1 as an alternative means of H<sub>2</sub> production. Amine-coated silica particles were effective in immobilizing T. onnurineus NA1 by electrostatic interaction, showing a maximum cell adsorption capacity of 71.7 mg-dried cells per g of particle. In three cycles of repeated-batch cultivation using sodium formate as the sole energy source, immobilized cells showed reproducible H<sub>2</sub> production with a considerable increase in the initial production rate from 2.3 to 4.0 mmol l<sup>−1</sup> h<sup>−1</sup>, mainly due to the increase in the immobilized cell concentration as the batch culture was repeated. Thus, the immobilized-cell system of T. onnurineus NA1 was demonstrated to be feasible for H<sub>2</sub> production. This study is the first example of immobilized cells of hyperthermophilic archaea being used for the production of H<sub>2</sub>.
Teratogenic Effects of Nano- and Micro-sized Particles of Zinc Oxide during Mouse Organogenesis
Sang-Yoon Nam , Jung-Min Yon, A Young Jung, Chunmei Lin, Jong-Geol Lee, Ki Youn Jung, Hansung Na, Myeon-Woo Chung, Beom Jun Lee, Young Won Yun 충북대학교 동물의학연구소 2011 Journal of Biomedical and Translational Research Vol.12 No.2
Zinc oxide nanoparticles (nZnO) are used in a various range, including ceramic manufacture, photocatalysis, UV filters, and the food industry. However, little is known about the effects of micro- and nano-particles during mouse embryo organogenesis. To determine whether ZnO affects size-dependent anomalies during embryonic organogenesis, mouse embryos were cultured for two days with 300 ug/ml micro ZnO (mZnO;80±25 μm) and nZnO (< 100 nm) and the developmental changes were then investigated. Quantity of Zn by inductively coupled plasma mass spectrometry analysis, and expression patterns of various antioxidant enzymes in the embryos were investigated. Embryos exposed to mZnO or nZnO exhibited severe retardation of growth and development. In embryos exposed to mZnO and nZnO, yolk sac diameter, crown-rump length, and head length were significantly diminished. The morphological parameters, including yolk sac circulation, allantois, flexion, heart, hindbrain, midbrain, forebrain, otic system, optic system, branchial bars, maxillary process, mandibular process, olfactory system, caudal neural tube, forelimb, hindlimb, and somites in mZnO and nZnO-treated groups were significantly decreased. Zn absorption of the nZnO-treated group was significantly higher than that of the mZnO-treated group. Significantly decreased levels of CuZn-SOD, Mn-SOD, cGPx, and PHGPx mRNA were observed in the ZnO-treated group. In addition, antioxidant enzyme mRNA expressions of the nZnO group were significantly diminished, less than those of the mZnO treated group. These findings indicate that 300 ug/ml ZnO showed abnormality and nZnO may have a more severe effect than mZnO in developing embryos.
Hoeflea halophila sp. nov., a novel bacterium isolated from marine sediment of the East Sea, Korea.
Jung, Man-Young,Shin, Kee-Sun,Kim, Suyeon,Kim, So-Jeong,Park, Soo-Je,Kim, Jong-Geol,Cha, In-Tae,Kim, Mi-Na,Rhee, Sung-Keun N.V. Swets en Zeitlinger 2013 Antonie van Leeuwenhoek Vol.103 No.5
<P>A Gram-negative, aerobic, motile, straight or curved rod-shaped marine bacterium was isolated from marine sediment of the East Sea, Korea. The isolated strain, JG120-1(T), grows with 0-5 % (w/v) NaCl and at 15-30 C and pH 6-9. α-galactosidase activity test was positive. Comparative 16S rRNA gene sequence studies showed that this strain belonged to the Alphaproteobacteria and was the most closely related to Hoeflea alexandrii AM1 V30(T), Hoeflea phototrophica DFL-43(T) and Hoeflea marina LMG 128(T) (98.9, 97.9 and 97.0 % 16S rRNA gene sequence similarities, respectively). Strain JG120-1(T) was found to possess summed feature 8 (C18:1ω7c/C18:1ω6c, 71.11 %) as the major cellular fatty acid. The major ubiquinone was determined to be Q-10. Polar lipids include phosphatidylglycerol, phosphatidylethanolamine, sulfoquinovosyl diacylglycerol, phosphatidylcholine and phosphatidylmonomethylethanolamine. The G+C content of the genomic DNA of strain JG120-1(T) was determined to be 57.8 mol %. DNA-DNA relatedness data indicated that strain JG120-1(T) represents a distinct species that is separate from H. phototrophica DFL-43(T), H. marina LMG128(T) and H. alexandrii AM1 V30(T). On the basis of polyphasic evidences, it is proposed that strain JG120-1(T) (= KCTC 23107(T) = JCM 16715(T)) represents the type strain of a novel species, Hoeflea halophila sp. nov.</P>
Na, Hee-Kyung,Wi, Jung-Sub,Son, Hye Young,Ok, Jong G.,Huh, Yong-Min,Lee, Tae Geol Elsevier 2018 Biosensors & bioelectronics Vol.113 No.-
<P><B>Abstract</B></P> <P>Localized surface plasmon resonance (LSPR) biosensors have attracted much interest due to their capacity for multiplexing, miniaturization, and high performance, which offers the potential for their integration into lab-on-a-chip platforms for point-of-care (POC) diagnostics. The need for microRNA (miRNA)-sensing platforms is particularly urgent because miRNAs are key regulators and biomarkers in numerous pathological processes and diseases. Unfortunately, however, development of such miRNA-sensing platforms has not yet been achieved. In order to realize the detection of these important biomarkers, there has been an increasing demand for POC-sensing platforms that enable label-free quantification with low sample consumption, good sensitivity, real-time responsiveness, and high throughput. Here, we developed a highly specific, sensitive LSPR miRNA-sensing platform on a flexible, scalable plasmonic nanostructure to enable single-base mismatch discrimination and attomole detection of miRNAs in clinically relevant samples. The hairpin probe contained a locked nucleic acid (LNA) that enabled the discrimination of single base mismatches based on differences in melting temperatures of perfectly matched or single base mismatched miRNAs when they formed base pairs with probes. In addition, through hybridization induced signal amplification based on precipitate formation on the gold surface through the enzyme reaction, we observed a dramatic LSPR peak shift, which enabled attomole detection. Additionally, our LSPR miRNA sensor enabled the detection of <I>miR-200a-3p</I> in total RNA extracts from primary cancer cell lines without purification or labeling of the miRNA. This label-free and highly specific miRNA sensing platform may have applications in POC cancer diagnostics without the need for gene amplification.</P> <P><B>Highlights</B></P> <P> <UL> <LI> Signal amplification strategy was developed for sensitive miRNA detection on a scalable three dimensional gold nanostructure. </LI> <LI> Localized surface plasmon resonance (LSPR) miRNA sensor was able to discriminate single base mismatches. </LI> <LI> Signal amplification enabled the largest LSPR peak shift in the presence of the target miRNA. </LI> <LI> Sensitive detection of miRNAs in the biological samples was demonstrated. </LI> </UL> </P>
Effect of Chuna (Shoulder Traction) Treatment on Frozen Shoulder During Korean Medical Treatment
Jo, Na Young,Yeo, In Ho,Jung, Se Ho,Sung, Hee Jin,Lee, Cham Geol,Lee, Eun Yong,Roh, Jeong Du Korean AcupunctureMoxibustion Medicine Society 2014 대한침구의학회지 Vol.21 No.1
Objectives : Through an analysis of the previous studies, It is estimated that Chuna(shoulder traction) is effective in reducing the pain and increasing motion range of shoulder joint of the patient. So this study is to investigate the effects of Chuna(shoulder traction) on frozen shoulder. Methods : After treated with acupuncture and electro stimulating therapy, the subjects were measured list of measurement. And then treated with Chuna(shoulder traction) therapy, the subjects were measured list of measurement again. All treatment was performed by the same doctor who is a highly qualified about Chuna(shoulder traction). Chuna(shoulder traction) treatment takes 15 minutes. Three kind of Chuna(shoulder traction) therapy were performed for 5 minutes each. Depending on the degree of the subject's pain, treatment strength was adjusted. Results : - Chuna simultaneous treatment group, shoulder joint range of flexion, extension, abduction, adduction, external rotation and internal rotation movement improved statistically significant. - Chuna simultaneous and acupuncture treatment group, visual analogue scale(VAS) scores of two groups decreased statistically significant. Conclusions : The result suggests that Chuna(shoulder traction) can be effective to recover range of motion and reduce pain on patients with frozen shoulder.
Seo, Jung Yoon,Praveenkumar, Ramasamy,Kim, Bohwa,Seo, Jeong-Cheol,Park, Ji-Yeon,Na, Jeong-Geol,Jeon, Sang Goo,Park, Seung Bin,Lee, Kyubock,Oh, You-Kwan The Royal Society of Chemistry 2016 GREEN CHEMISTRY Vol.18 No.14
<P>Microalgal biofuel, albeit an exciting potential fossil-fuel-replacement candidate, still requires the development of more advanced downstream processing technology for its price competitiveness. The major challenge in a microalgae-based biorefinery is the efficient separation of microalgae from low-concentration culture broth. The post-harvesting cell-disruption step necessary to render microalgae suitable for lipid extraction, moreover, further raises energy consumption and cost. For the mitigation of biorefinery complexity and costs, we suggest herein a new scheme that integrates the critical downstream processes (harvesting and cell disruption) by means of cationic surfactant-decorated Fe3O4 nanoparticles. The cationic surfactants' quaternary ammonium heads play an important role in not only flocculating negatively charged microalgae but also weakening thick cell walls. In the present study, the harvesting efficiency and cell-damaging effects of three cationic surfactants - cetrimonium bromide (CTAB), cetylpyridinium chloride (CPC), and cetylpyridinium bromide (CPB) - were evaluated. The CTAB-decorated Fe3O4 nanoparticles, which were found to be the most effective, achieved a 96.6% microalgae harvesting efficiency at a dosage of 0.46 g particle per g cell. Next, for the purposes of magnetic nanoparticle recycling and high-purity microalgal biomass obtainment, microalgae detachment from microalgae-Fe3O4 flocs was performed by addition of an anionic surfactant, sodium dodecyl sulfate (SDS). The detached CTAB-decorated Fe3O4 nanoparticles showed a steady reuse efficiency of about 80%. Furthermore, microalgae harvesting by CTAB-decorated Fe3O4 nanoparticles could contribute to a great improvement in the total extracted lipid content and greener wet extraction without the additional energy-intensive cell-disruption step, thus demonstrating the cell-disruption ability of CTAB-decorated Fe3O4 nanoparticles.</P>