개선된 중성염 전해공정을 이용한 모의 방사성금속폐기물의 제염

이지훈,육완이,양호연,하종현 대한방사선 방어학회 2002 방사선방어학회지 Vol.27 No.2

원자력발전소에서 주로 발생되는 금속폐기물인 탄소강을 중성염전해질인 1.7M의 황산나트륨(Na₂SO₄)과 질산나트륨(NaNO₃)을 이용하여 기존전해제염과 개선된 전해제염공정의 비교실험을 수행하였다. 양극은 인코넬, 음극은 티타늄으로 하여 상온에서 1시간동안 반응시켜 금속폐기물 모재의 weight loss, 두께변화, 전해질 내 침전물농도, SEM을 이용하여 제염전후의 금속폐기물 표면의 형상을 분석하였다. 실험결과 개선된 전해제염 적용시 전해질 종류별 전류밀도 변화에 따른 실험에서는 전류밀도가 0.1∼0.6A/cm²으로 증가함에 따라 1.7M의 황산나트륨 적용시 금속폐기물 모재의 두께변화는 0.48±0.005∼67.7±0.02um, 1.7M의 질산나트륨 적용시에는 0.06±0.005∼17.7±0.05로 나타나 같은 전류밀도에서 황산나트륨 적용시 금속폐기물 모재의 표면 제염효율이 더욱 높은 양상을 보였다. 또한 전류밀도 0.3 A/cm² 및 1.7M의 황산나트륨의 조건에서 개선된 전해제염 적용 시 9.8±0.01um의 금속폐기물 두께변화를 보여 기존전해제염 적용시인 3.7±0.03um의 금속폐기물 두께변화보다 2배 이상의 표면 제염효과를 보였다. Conventional and modified electrolytic decontamination experiment were performed in the 1.7 M solution of sodium sulfate and sodium nitrate for decontamination of carbon steel as the simulated metal wastes which have been produced in large amounts from nuclear power plants. Anode and cathode were used as inconel and titanium respectively. The reaction time and temperature were 1 hr and 25℃. The analyses were performed of the characteristics such as weight loss and thickness change of metal waste, suspended solid in electrolyte and SEM observation. In modified electrolyte decontamination system with increased current density ranged from 0.1 to 0.6 A/cm², the metal waste showed thickness changes of 0.48±0.005 to 67.7±0.02um in 1.7 M sodium sulfate and those of 0.06±0.005 to 17.7±0.05um in sodium nitrate. Metal waste in modified electrolyte decontamination system showed the thickness change of 9.8±0.01um while it reacted up to 3.7±0.03um in conventional system with 0.3 A/cm² of current density and 1.7 M sodium sulfate. Decontamination efficiencies of modified electrolytic process are much higher than that of conventional electrolytic process when both are applied to metal waste.

單症性 健康念慮症의 臨床經驗

韓辰熙,柳太烈,趙伯紀,李鍾六 서울醫大 1990 精神醫學 Vol.15 No.2

The Effects of Thermal Injury on Epidermal Langerhans Cells and Contact Hypersensitivity in Guinea Pigs

Yi, Jong-Yuk,Jun, Kyung-Ho,Cho, Baik-Kee,Houh, Woo Catholic Medical Center 1990 Bulletin of the Clinical Research Institute Vol.18 No.1

Two Cases of Type V Hyperlipoproteinemia and Eruptive Xanthomas associated with Diabetes Mellitus

Yi, Jong Yuk,Park, Chul Jong,Mo, Hyun Jin,Son, Hyun Shik 대한피부과학회 2001 Annals of Dermatology Vol.13 No.3

We report two cases of type V hyperlipoproteinemia and eruptive xanthomas associated with diabetes mellitus in a 44-year-old woman and a 25-year-old woman. They had asymptomatic, diffuse, erythematous yellowish papules on the extremities and buttocks. The laboratory studies showed increased serum blood glucose, total cholesterol, triglyceride, increasing pre-β and chylomicron bands on electrophoresis of lipoprotein. Histopathologic findings of erythematous yellowish papules on the thigh and buttock revealed aggregation of foam cells in the dermis. They have been treated with diet control, hypoglycemic agent (gliclazide) and hypolipidemic drug (gemfibrozil), and are under continued supervision.

The Expression of c - erbB - 1 and c - erbB - 2 in the Various Skin Tumors

Yi, Jong Yuk,Park, Chul Jong,Ham, Sang Hee 대한피부과학회 2001 Annals of Dermatology Vol.13 No.2

Background : c-erbB-1 (epidermal growth factor receptor) and c-erbB-2 oncoprotein have common tyrosine kinase activities, and alteration in their expression has been defined in various visceral tumors. However, relatively little is known about their expression in skin tumors. Objective : Our aim was to evaluate the distribution and expression pattern of c-erbB-1 and c-erbB-2 in skin neoplasms. Methods : We have undertaken an immunohistochemical survey of c-erbB-1 and c-erbB-2 in the tissue specimens of keratoacanthoma (KA), actinic keratosis (AK), squamous cell carcinoma (SCC) and basal cell carcinoma (BCC). Results : Membranous c-erbB-1 expression had a tendency to be down-regulated in some specimens of SCC and dysplastic portions of AK. In invasive lesions of SCC, we observed increased cytoplasmic accumulation of c-erbB-1. Most specimens of BCC showed rather decreased expression of c-erbB-1 compared with other skin tumors. c-erbB-2 oncoprotein showed strong cytoplasmic staining in SCC, especially in the invasive tumor mass, and in some deeply dysplastic or hyperplastic portions of AK, though the difference of intensity was not striking between tumors. BGC revealed relatively weaker expression of c-erbB-2 than other skin tumors, which was similar in pattern to c-erbB-1. Conclusion : The expression patterns of c-erbB-1 and c-erbB-2 are altered in various skin neoplasms, and seem to be related to the dysplastic status or differentiation level of tumor cells.

Immunohistochemical Detection of p16 , p21 , and TGF - β in Cutaneous Epithelial Tumors

Yi, Jong Yuk,Kim, Si Yong,Park, Chul Jong,Park, Kee Woan 대한피부과학회 2001 Annals of Dermatology Vol.13 No.1

Background : It has been known that p16, p21, and TGF-β are related to cellular proliferation and malignant transformation but the results of the previous studies are controversial. Objective : This study was performed in order to investigate the possible role of p16, p21, and TGF-β in relation to the cellutar proliferation and malignant transformation in various skin tumors. Methods : For immunohistochemical staining we examined sections (4um thick) of formalin-fixed, paraffin-embedded tissue from 11 cases of squamous cell carcinoma (SCC), 13 cases of basal cell carcinoma (BCC), 7 cases of actinic keratosis, 5 cases of keratoacanthoma, and 4 cases of normal skin for control. Following conventional deparaffinization, the three step immunoperoxidase method was performed using the streptavidine-biotin complex and monoclonal antibodies. All sections were counterstained with hematoxylin. The expression of p16, p21, and TGF-β was evaluated and graded in relation to the intensity of cytoplasmic immunostaining of positive epidermal cells. Results : p16, p21, and TGF-β were detected mainly in the cytoplasm. The p21 positive cells were localized exclusively in the upper squamous layer and were not visualized in the basal layer. 'The expression of p21 was higher in SCC than BCC (P value=0.03). The expression of p21 in normal control was higher than skin tumors, but there was no significant difference among these tumors. There was a significant loss in the expression of p16 and TGF-β in various skin tumors compared with normal control, but no difference in the expression of p16 and TGF-β among these skin tumors. Conclusion : 1. p21 is possibly related to the differentiation of epidermal cells, because p21 was observed not in basal cells but in squamous and granular cells of the normal epidermis and the higher expression of p21 was demonstrated in SCC compared to BCC. 2. It may imply that loss of expression of p16 may play a critical role in malignant transformation but not in tumor progression of human malignant skin tumors, because the significant loss of the expression of p16 was found in SCC and BCC when normal skin and there was no difference in the expression of p16 among various skin tumors but the expression of p16 of SCC is higher than that of actinic keratosis (P value=0.04). 3. It may imply that the loss of the expression of TGF-R may play a critical role in malignant transformation, because the significant loss of expression of TGF-β was found in various skin tumors when compared to normal skin, but there was no difference in the expression of TGF-β among various skin tumors.

Assessment of IgE Detecting Methods in Cord and Maternal Serum

Yi, Jong Yuk,Park, Chun Wook,Kim, Hyung Ok,Kim, Chung Won CATHOLIC MEDICAL CENTER 1985 Bulletin of the Clinical Research Institute Vol.13 No.1

The aim of the present work is to evaluate the most accurate technique for analysing low levels of serum IgE. Following results are obtained from experiments by PRIST, Overnight PRIST, PRIST using RAST anti-IgE tracer and Overnight PRIST using RAST anti-IgE tracer methods with cord serum and maternal serum of 21 individuals respectively. 1. There was no statistical significance in maternal serum IgE liter using above 4 methods. However, the higher level of IgE titer was found in PRIST method when IgE was over 190 IU/ml (5 cases) estimated by PRIST method and in Overnight PRIST using RAST anti-IgE tracer method when IgE was below 190 IU/ml (16 cases) (P<0.05). 2. In cord serum IgE titer, only Overnight PRIST using RAST anti-IgE tracer method showed statistical significance. 3. The most desirable correlation coefficient between maternal serum and cord serum IgE titer was estimated by Overnight PRIST using RAST anti-IgE tracer method than any other methods.

The Systemic Effect of Local Hyperthermia on Contact Hypersensitivity in Mice

Yi, Jong Yuk,Cho, Baik Kee,Houh, Won CATHOLIC MEDICAL CENTER 1991 Bulletin of the Clinical Research Institute Vol.19 No.1

This study was designed to investigate the systemic suppressive effect of local hyperthermia on contact hypersensitivity (CHS) and elucidate its mechanism through transfer of spleen cells. Depilated dorsal skin was immersed into a controlled water bath (at 52℃ for 30 seconds). 2,4-dinitro-1-fluoro-benzene (DNFB) and 4-ethoxyrnethylene-2-oxazolin-5-one (oxazolone) was used for sensitization and challenge. Spleen cells obtained from hyporesponsive mice after hyperthermia treatment and DNFB sensitization, and the positive control group, which did not receive hyperthermia treatment but were sensitized, were injected intravenously into normal syngeneic mice. The results were as follows: 1. CHS was significantly suppressed in mice sensitized on day 5 or day 7 after hyperthermia treatment at the untreated skin (p<0.05, p<0.01), but it was meagerly suppressed in mice sensitized on day 1, day 3 or day 10 after hyperthermia treatment. 2. When the mice received spleen cells obtaind from hyporesponsive donors sensitized on day 7 after hyperthermia treatment before sensitization, CHS of the recipient mice was significantly suppressed (p<0.05). When they received spleen cells obtained from hyporesponsive donors sensitized on day 5 after hyperthermia treatment, CHS of the recipient mice was suppressed, but not significantly. 3. When spleen cells obtained from hyporesponsive donors sensitized on day 7 after hyperthermia treatment were transferred after the recipient mice were sensitized, CHS of the recipient mice was not suppressed. 4. When spleen cells obtained from hyporespensive donors sensitized with DNFB on day 7 after hyperthermia treatment were transferred before the recipient mice were sensitized with oxazolone, the suppression of CHS in the recipient mice was not observed. These findings suggest that local hyperthermia treatment suppresses CHS systemically in mice and this systemic suppressive effect is at least in part associated with the induction of antigen-specific suppressor cells which block the afferent limb of CHS but not the efferent limb.

Assessmints of in Vivo and in Vitro Tests for Atopic Diseases

Yi, Jong-Yuk,Kim, Jin-Wou,Kim, Hyung-Ok,Kim, Chung-Won Catholic Medical Center 1987 Bulletin of the Clinical Research Institute Vol.15 No.-

The Effects of Thermal Injury on Epidermal Langerhans Cells amd Contact Hypersensitivity in Guinea Pigs

Yi, Jong Yuk,Jun, Kyung Ho,Cho, Baik Kee,Houh, Won CATHOLIC MEDICAL CENTER 1990 Bulletin of the Clinical Research Institute Vol.18 No.1

This study was attempted to investigate the effects of thermal injury on epidermal Langerhans cell (LC) and the induction of contact hypersensitivity (CHS), and the relevance of the density of epidermal LC to tile degree of CHS in guinea pigs. The skin was treated with 65℃ for 5 seconds. Epidermal LC count utilized ATPase staining and DNCB (2, 4-dinitrochlor-obenzene) was used as sensitizer. The results were as follows: 1. The number of epidermal to was significantly reduced on day 0, day 5 day 10 and day 15 after thermal injury compared with that of 5 days before thermal injury (p<0.01). 2. CHS was significantly suppressed in groups sensitized on day 0 (group A), day 5 (group B) and day 10 (group C) after thermal injury compared with positive control group (group E) (P < 0.01). 3. CHS was correlated relatively with the density of epidermal LC. 4. Reduced number of epidermal LC and suppressed CHS were recovered to normal 15-20 days after therma1 injury.