mRNA Expression of Ovine Angiopoietin-like Protein 4 Gene in Adipose Tissues

Zhang, Jing,Jing, Jiong-Jie,Jia, Xia-Li,Qiao, Li-Ying,Liu, Jian-Hua,Liang, Chen,Liu, Wen-Zhong Asian Australasian Association of Animal Productio 2016 Animal Bioscience Vol.29 No.5

Angiopoietin-like protein 4 (ANGPTL4) is involved in a variety of functions, including lipoprotein metabolism and angiogenesis. To reveal the role of ANGPTL4 in fat metabolism of sheep, ovine ANGPTL4 mRNA expression was analyzed in seven adipose tissues from two breeds with distinct tail types. Forty-eight animals with the gender ratio of 1:1 for both Guangling Large Tailed (GLT) and Small Tailed Han (STH) sheep were slaughtered at 2, 4, 6, 8, 10, and 12 months of age, respectively. Adipose tissues were collected from greater and lesser omental, subcutaneous, retroperitoneal, perirenal, mesenteric, and tail fats. Ontogenetic mRNA expression of ANGPTL4 in these adipose tissues from GTL and STH was studied by quantitative real time polymerase chain reaction. The results showed that ANGPTL4 mRNA expressed in all adipose tissues studied with the highest in subcutaneous and the lowest in mesenteric fat depots. Months of age, tissue and breed are the main factors that significantly influence the mRNA expression. These results provide new insights into ovine ANGPTL4 gene expression and clues for its function mechanism.

MiR-188-5p regulates the proliferation and differentiation of goat skeletal muscle satellite cells by targeting calcium/calmodulin dependent protein kinase II beta

Jing Jing,Sihuan Zhang,Jinbo Wei,Yuhang Yang,Qi Zheng,Cuiyun Zhu,Shuang Li,Hongguo Cao,Fugui Fang,Yong Liu,Ying-hui Ling Asian Australasian Association of Animal Productio 2023 Animal Bioscience Vol.36 No.12

Objective: The aim of this study was to reveal the role and regulatory mechanism of miR-188-5p in the proliferation and differentiation of goat muscle satellite cells. Methods: Goat skeletal muscle satellite cells isolated in the pre-laboratory were used as the test material. First, the expression of miR-188-5p in goat muscle tissues at different developmental stages was detected by quantitative reverse transcription polymerase chain reaction (qRT-PCR). In addition, miR-188-5p was transfected into goat skeletal muscle satellite cells by constructing mimics and inhibitors of miR-188-5p, respectively. The changes of differentiation marker gene expression were detected by qPCR method. Results: It was highly expressed in adult goat latissimus dorsi and leg muscles, goat fetal skeletal muscle, and at the differentiation stage of muscle satellite cells. Overexpression and interference of miR-188-5p showed that miR-188-5p inhibited the proliferation and promoted the differentiation of goat muscle satellite cells. Target gene prediction and dual luciferase assays showed that miR-188-5p could target the 3'untranslated region of the calcium/calmodulin dependent protein kinase II beta (CAMK2B) gene and inhibit luciferase activity. Further functional studies revealed that CAMK2B promoted the proliferation and inhibited the differentiation of goat muscle satellite cells, whereas si-CAMK2B restored the function of miR-188-5p inhibitor. Conclusion: These results suggest that miR-188-5p inhibits the proliferation and promotes the differentiation of goat muscle satellite cells by targeting CAMK2B. This study will provide a theoretical reference for future studies on the molecular mechanisms of skeletal muscle development in goats.

MiRNA320a Inhibitor-Loaded PLGA-PLL-PEG Nanoparticles Contribute to Bone Regeneration in Trauma-Induced Osteonecrosis Model of the Femoral Head

Zhang Ying,Li Chuan,Wei Qiushi,Yuan Qiang,He Wei,Zhang Ning,Dong Yiping,Jing Zhenhao,Zhang Leilei,Wang Haibin,Cao Xiangyang 한국조직공학과 재생의학회 2024 조직공학과 재생의학 Vol.21 No.1

BACKGROUND: This study aimed to explore the effect of a nanomaterial-based miR-320a inhibitor sustained release system in trauma-induced osteonecrosis of the femoral head (TIONFH). METHODS: The miR-320a inhibitor-loaded polyethylene glycol (PEG)- Poly(lactic-co-glycolic acid) (PLGA)- Poly-L-lysine (PLL) nanoparticles were constructed using the double emulsion method. The TIONFH rabbit model was established to observe the effects of miR-320a inhibitor nanoparticles in vivo. Hematoxylin–eosin staining and microcomputed tomography scanning were used for bone morphology analysis. Bone marrow mesenchymal stem cells (BMSCs), derived from TIONFH rabbits, were used for in vitro experiments. Cell viability was determined using the MTT assay. RESULTS: High expression of miR-320a inhibited the osteogenic differentiation capacity of BMSCs in vitro by inhibiting the expression of the osteoblastic differentiation markers ALP and RUNX2. MiR-320a inhibitor-loaded PEG-PLGA-PLL nanoparticles were constructed with a mean loading efficiency of 1.414 ± 0.160%, and a mean encapsulation efficiency of 93.45 ± 1.24%, which released 50% of the loaded miR-320a inhibitor at day 12 and 80% on day 18. Then, inhibitor release entered the plateau. After treatment with the miR-320a inhibitor nanoparticle, the empty lacunae were decreased in the femoral head tissue of TIONFH rabbits, and the osteoblast surface/bone surface (Ob.S/BS), osteoblast number/bone perimeter (Ob.N/B.Pm), bone volume fraction, and bone mineral density increased. Additionally, the expression of osteogenic markers RUNX2 and ALP was significantly elevated in the TIONFH rabbit model. CONCLUSION: The miR-320a inhibitor-loaded PEG-PLGA-PLL nanoparticle sustained drug release system significantly contributed to bone regeneration in the TIONFH rabbit model, which might be a promising strategy for the treatment of TIONFH. BACKGROUND: This study aimed to explore the effect of a nanomaterial-based miR-320a inhibitor sustained release system in trauma-induced osteonecrosis of the femoral head (TIONFH). METHODS: The miR-320a inhibitor-loaded polyethylene glycol (PEG)- Poly(lactic-co-glycolic acid) (PLGA)- Poly-L-lysine (PLL) nanoparticles were constructed using the double emulsion method. The TIONFH rabbit model was established to observe the effects of miR-320a inhibitor nanoparticles in vivo. Hematoxylin–eosin staining and microcomputed tomography scanning were used for bone morphology analysis. Bone marrow mesenchymal stem cells (BMSCs), derived from TIONFH rabbits, were used for in vitro experiments. Cell viability was determined using the MTT assay. RESULTS: High expression of miR-320a inhibited the osteogenic differentiation capacity of BMSCs in vitro by inhibiting the expression of the osteoblastic differentiation markers ALP and RUNX2. MiR-320a inhibitor-loaded PEG-PLGA-PLL nanoparticles were constructed with a mean loading efficiency of 1.414 ± 0.160%, and a mean encapsulation efficiency of 93.45 ± 1.24%, which released 50% of the loaded miR-320a inhibitor at day 12 and 80% on day 18. Then, inhibitor release entered the plateau. After treatment with the miR-320a inhibitor nanoparticle, the empty lacunae were decreased in the femoral head tissue of TIONFH rabbits, and the osteoblast surface/bone surface (Ob.S/BS), osteoblast number/bone perimeter (Ob.N/B.Pm), bone volume fraction, and bone mineral density increased. Additionally, the expression of osteogenic markers RUNX2 and ALP was significantly elevated in the TIONFH rabbit model. CONCLUSION: The miR-320a inhibitor-loaded PEG-PLGA-PLL nanoparticle sustained drug release system significantly contributed to bone regeneration in the TIONFH rabbit model, which might be a promising strategy for the treatment of TIONFH.

A Genetic Variant in MiR-146a Modifies Digestive System Cancer Risk: a Meta-analysis

Li, Ying-Jun,Zhang, Zhen-Yu,Mao, Ying-Ying,Jin, Ming-Juan,Jing, Fang-Yuan,Ye, Zhen-Hua,Chen, Kun Asian Pacific Journal of Cancer Prevention 2014 Asian Pacific journal of cancer prevention Vol.15 No.1

MicroRNAs (miRNAs) negatively regulate gene expression and act as tumor suppressors or oncogenes in oncogenesis. The association between a single nucleotide polymorphism (SNP) in miR-146a rs2910164 and susceptibility to digestive system cancers was inconsistent in previous studies. In this study, we conducted a literature search of PubMed to identify all relevant studies published before August 31, 2013. A total of 21 independent case-control studies were included in this updated meta-analysis with 9,558 cases and 10,614 controls. We found that the miR-146a rs2910164 polymorphism was significantly associated with decreased risk of digestive system cancers in an allele model (OR=0.90, 95%CI 0.87-0.94), homozygote model (OR=0.84, 95%CI 0.77-0.91), dominant model (OR=0.90, 95%CI 0.84-0.96), and recessive model (OR=0.85, 95%CI 0.79-0.91), while in a heterozygous model (OR = 0.99, 95% CI 0.89-1.11) the association showed marginal significance. Subgroup analysis by cancer site revealed decreased risk in colorectal cancer above allele model (OR=0.90, 95%CI 0.83-0.97) and homozygote model (OR=0.85, 95%CI 0.72-1.00). Similarly, decreased cancer risk was observed when compared with allele model (OR=0.87, 95%CI 0.81-0.93) and recessive model (OR=0.81, 95%CI 0.72-0.90) in gastric cancer. When stratified by ethnicity, genotyping methods and quality score, decreased cancer risks were also observed. This current meta-analysis indicated that miR-146a rs2910164 polymorphism may decrease the susceptibility to digestive system cancers, especially in Asian populations.

Inhibitory effects of piceatannol on human cytomegalovirus (hCMV) in vitro

Wang San-Ying,Zhang Jing,Xu Xiao-Gang,Su Hui-Li,Xing Wen-Min,Zhang Zhong-Shan,Jin Wei-Hua,Dai Ji-Huan,Wang Ya-Zhen,He Xin-Yue,Sun Chuan,Yan Jing,Mao Gen-Xiang 한국미생물학회 2020 The journal of microbiology Vol.58 No.8

Human cytomegalovirus (hCMV) is a ubiquitous herpesvirus, which results in the establishment of a latent infection that persists throughout the life of the host and can be reactivated when the immunity is low. Currently, there is no vaccine for hCMV infection, and the licensed antiviral drugs mainly target the viral enzymes and have obvious adverse reactions. Thus, it is important to search for compounds with antihCMV properties. The present study aimed to investigate the suppressive effects of piceatannol on hCMV Towne strain infection and the putative underlying mechanisms using human diploid fibroblast WI-38 cells. Piceatannol supplementation prevented the lytic changes induced by hCMV infection in WI-38 cells. Furthermore, piceatannol suppressed the expression of hCMV immediate-early (IE) and early (E) proteins as well as the replication of hCMV DNA in a dose-dependent manner. Moreover, hCMV-induced cellular senescence was suppressed by piceatannol, as shown by a decline in the senescence-associated β-galactosidase (SA-β-Gal) activity and decreased production of intracellular reactive oxygen species (ROS). p16INK4a, a major senescence-associated molecule, was dramatically elevated by current hCMV infection that was attenuated by pre-incubation with piceatannol in a dose-dependent manner. These results demonstrated that piceatannol suppressed the hCMV infection via inhibition of the activation of p16INK4a and cellular senescence induced by hCMV. Together, these findings indicate piceatannol as a novel and potent anti-hCMV agent with the potential to be developed as an effective treatment for chronic hCMV infection.

Genetic Analysis of the Liver Putative Tumor Suppressor (LPTS) Gene in Gastric Cancer

Hai Bing Zhang,Qu Zhang,Li Sa Wang,Fei Yan,Yun Lu,Zhi Qiang Wang,Jing Li,Liang Liang Zhang,Ying Yan Yu,Zheng Gang Zhu,Xiang Yin Kong,Lan Dian Hu 한국유전학회 2005 Genes & Genomics Vol.27 No.4

20(S)-ginsenoside Rh2 ameliorates ATRA resistance in APL by modulating lactylation-driven METTL3

Siyu Cheng,Langqun Chen,Jiahui Ying,Ying Wang,Wenjuan Jiang,Qi Zhang,Hong Zhang,Jiahe Wang,Chen Wang,Huimin Wu,Jing Ye,Liang Zhang 고려인삼학회 2024 Journal of Ginseng Research Vol.48 No.3

Background: 20(S)-ginsenoside Rh2(GRh2), an effective natural histone deacetylase inhibitor, can inhibit acute myeloid leukemia (AML) cell proliferation. Lactate regulated histone lactylation, which has different temporal dynamics from acetylation. However, whether the high level of lactylation modification that we first detected in acute promyelocytic leukemia (APL) is associated with all-trans retinoic acid (ATRA) resistance has not been reported. Furthermore, Whether GRh2 can regulate lactylation modification in ATRA-resistant APL remains unknown. Methods: Lactylation and METTL3 expression levels in ATRA-sensitive and ATRA-resistant APL cells were detected by Western blot analysis, qRT-PCR and CO-IP. Flow cytometry (FCM) and APL xenograft mouse models were used to determine the effect of METTL3 and GRh2 on ATRA-resistance. Results: Histone lactylation and METTL3 expression levels were considerably upregulated in ATRA-resistant APL cells. METTL3 was regulated by histone lactylation and direct lactylation modification. Overexpression of METTL3 promoted ATRA-resistance. GRh2 ameliorated ATRA-resistance by downregulated lactylation level and directly inhibiting METTL3. Conclusions: This study suggests that lactylation-modified METTL3 could provide a promising strategy for ameliorating ATRA-resistance in APL, and GRh2 could act as a potential lactylation-modified METTL3 inhibitor to ameliorate ATRA-resistance in APL.

Catalytic topological insulator Bi₂Se₃ nanoparticles for <i>in</i> <i>vivo</i> protection against ionizing radiation

Zhang, Xiao-Dong,Jing, Yaqi,Song, Shasha,Yang, Jiang,Wang, Jun-Ying,Xue, Xuhui,Min, Yuho,Park, Gyeongbae,Shen, Xiu,Sun, Yuan-Ming,Jeong, Unyong Elsevier 2017 Nanomedicine Vol.13 No.5

<P><B>Abstract</B></P> <P>Bi<SUB>2</SUB>Se<SUB>3</SUB> nanoparticles (NPs) have attracted wide interests in biological and medical applications. Layer-like Bi<SUB>2</SUB>Se<SUB>3</SUB> with high active surface area is promising for free radical scavenging. Here, we extended the medical applications of Bi<SUB>2</SUB>Se<SUB>3</SUB> NPs further to <I>in vivo</I> protection against ionizing radiation based on their superior antioxidant activities and electrocatalytic properties. It was found that Bi<SUB>2</SUB>Se<SUB>3</SUB> NPs can significantly increase the surviving fraction of mice after exposure of high-energy radiation of gamma ray. Additionally, the Bi<SUB>2</SUB>Se<SUB>3</SUB> NPs can help to recover radiation-lowered red blood cell counts, white blood cell counts and platelet levels. Further investigations revealed that Bi<SUB>2</SUB>Se<SUB>3</SUB> NPs behaved as functional free radical scavengers and significantly decreased the level of methylenedioxyamphetamine. <I>In vivo</I> toxicity studies showed that Bi<SUB>2</SUB>Se<SUB>3</SUB> NPs did not cause significant side effects in panels of blood chemistry, clinical biochemistry and pathology.</P> <P><B>Highlights</B></P> <P> <UL> <LI> Bi<SUB>2</SUB>Se<SUB>3</SUB> NPs can significantly increase the surviving fraction of mice up to 70% after Gamma radiation. </LI> <LI> Bi<SUB>2</SUB>Se<SUB>3</SUB> NPs can help to recover radiation-lowered red blood cell counts, white blood cell counts and platelet levels. </LI> <LI> Bi<SUB>2</SUB>Se<SUB>3</SUB> NPs behaved as functional free radical scavengers and significantly decreased the level of methylenedioxyamphetamine. </LI> </UL> </P> <P><B>Graphical Abstract</B></P> <P>Layer-like Bi<SUB>2</SUB>Se<SUB>3</SUB> with high active surface area can protect mice against ionizing radiation based on their superior antioxidant activities and electrocatalytic properties. Bi<SUB>2</SUB>Se<SUB>3</SUB> NPs can significantly increase the surviving fraction of mice up to 70% after exposure of high-energy radiation of gamma ray. Bi<SUB>2</SUB>Se<SUB>3</SUB> NPs behaved as functional free radical scavengers and significantly decreased the level of methylenedioxyamphetamine. <I>In vivo</I> toxicity studies showed that Bi<SUB>2</SUB>Se<SUB>3</SUB> NPs did not cause significant side effects in panels of blood chemistry, clinical biochemistry and pathology.</P> <P>[DISPLAY OMISSION]</P>

Behavior of improved through-diaphragm connection to square tubular column under tensile loading

Ying Qin,Jing-Chen Zhang,Peng Shi,Yi-Fu Chen,Yao-Han Xu,Zuo-Zheng Shi 국제구조공학회 2018 Structural Engineering and Mechanics, An Int'l Jou Vol.68 No.4

Square tubular columns are commonly used in moment resisting frames, while through-diaphragm connection is the most typical configuration detail to connect the H-shaped beam to the column. However, brittle fracture normally occurs at the complete joint penetration weld between the beam flange and the through-diaphragm due to the stress concentration caused by the geometrical discontinuity. Accordingly, three improved types of through-diaphragm are presented in this paper to provide smooth force flow path comparing to that of conventional connections. Tensile tests were conducted on four specimens and the results were analyzed in terms of failure modes, load-displacement response, yield and ultimate capacity, and initial stiffness. Furthermore, strain distributions on the through-diaphragm, the beam flange plate, and the column face were comprehensively evaluated and discussed. It was found that all the proposed three types of improved through-diaphragm connections were able to reduce the stress concentration in the welds between the beam flange and the through-diaphragm. Furthermore, the stress distribution in connection with longer tapered through-diaphragm was more uniform.

Obesity-Associated Metabolic Signatures Correlate to Clinical and Inflammatory Profiles of Asthma: A Pilot Study

YING LIU,Jing Zheng,Hong-Ping Zhang,Xin Zhang,Lei Wang,Lisa Wood,Gang Wang 대한천식알레르기학회 2018 Allergy, Asthma & Immunology Research Vol.10 No.6

Purpose: Obesity is associated with metabolic dysregulation, but the underlying metabolic signatures involving clinical and inflammatory profiles of obese asthma are largely unexplored. We aimed at identifying the metabolic signatures of obese asthma. Methods: Eligible subjects with obese (n = 11) and lean (n = 22) asthma underwent body composition and clinical assessment, sputum induction, and blood sampling. Sputum supernatant was assessed for interleukin (IL)-1β, -4, -5, -6, -13, and tumor necrosis factor (TNF)-α, and serum was detected for leptin, adiponectin and C-reactive protein. Untargeted gas chromatography time-of-flight mass spectrometry (GC-TOF-MS)-based metabolic profiles in sputum, serum and peripheral blood monocular cells (PBMCs) were analyzed by orthogonal projections to latent structures-discriminate analysis (OPLS-DA) and pathway topology enrichment analysis. The differential metabolites were further validated by correlation analysis with body composition, and clinical and inflammatory profiles. Results: Body composition, asthma control, and the levels of IL-1β, -4, -13, leptin and adiponectin in obese asthmatics were significantly different from those in lean asthmatics. OPLS-DA analysis revealed 28 differential metabolites that distinguished obese from lean asthmatic subjects. The validation analysis identified 18 potential metabolic signatures (11 in sputum, 4 in serum and 2 in PBMCs) of obese asthmatics. Pathway topology enrichment analysis revealed that cyanoamino acid metabolism, caffeine metabolism, alanine, aspartate and glutamate metabolism, phenylalanine, tyrosine and tryptophan biosynthesis, pentose phosphate pathway in sputum, and glyoxylate and dicarboxylate metabolism, glycerolipid metabolism and pentose phosphate pathway in serum are suggested to be significant pathways related to obese asthma. Conclusions: GC-TOF-MS-based metabolomics indicates obese asthma is characterized by a metabolic profile different from lean asthma. The potential metabolic signatures indicated novel immune-metabolic mechanisms in obese asthma with providing more phenotypic and therapeutic implications, which needs further replication and validation.