Combined transcriptomic and proteomic analysis of flubendiamide resistance in Plutella xylostella

Li Jing‐Jing,Jin Ming‐Hui,Wang Nian‐Meng,Yu Qi‐Tong,Shang Ze‐Yu,Xue Chao‐Bin 한국곤충학회 2020 Entomological Research Vol.50 No.10

Diamondback moth (DBM), Plutella xylostella, is an important pest of crucifers worldwide. The extensive use of diamide insecticides has led to DBM resistance in the world, and this presents a serious threat to vegetable production. In the present study, transcriptomic and proteomic analyses were combined to investigate the potential flubendiamide‐resistance mechanism in DBM. The lab‐selected (Rh) and field‐collected (Rb) flubendiamide‐resistant lines of P. xylostella with resistance ratios of 1889.92‐fold and 1250.97‐fold, respectively, were used, as well as a lab‐reared flubendiamide‐susceptible line (S). Compared with the S group, the transcriptomic analysis revealed 151 upregulated and 287 downregulated gene messengers in the Rh group and 432 upregulated and 565 downregulated gene messengers in the Rb group. The most frequently enriched pathways of differentially expressed genes (DEGs) were mainly involved in metabolic pathways. Metabolism related genes, including two P450, two ABC transporters, and three trypsins, were upregulated in the Rh line. Additionally, some P450 genes, trypsin, juvenile hormone (JH), and mucin genes were also upregulated in the Rb line. In proteomic analysis comparisons with the S group, there were 78 upregulated and 90 downregulated proteins in the Rh group and 221 upregulated and 155 downregulated proteins in the Rb group. Further analyses found that three CYP and 11 CYP proteins were over‐expressed in Rh and Rb lines, respectively. Four glutathione S‐transferase (GST) and four UGTs were over‐expressed in Rb line. So, we deduced that the detoxification metabolism may be the main mechanism of flubendiamide resistance in P. xylostella.

What Frequency of Ankle Pump Exercise is Optimal to Improve Lower Limb Hemodynamics? A Systematic Review and Network Meta-analysis

Wang Xin,Tang Rongsong,Zhang Heli,Li Fan,Wang Jing,Li Baohua 한국간호과학회 2023 Asian Nursing Research Vol.17 No.2

Purpose: Ankle pump exercises (APE) have been widely used in clinical practice. However, best practices for APE have not been established. Recognize the most effective frequency of APE for improving lower extremity hemodynamics and establish recommendations in clinical practice. Methods: Therefore, a systematic review and network meta-analysis (NMA) was performed according to PRISMA-NMA. Six English databases (Pubmed, Medline, CINAHL, Embase, the Cochrane library and ProQuest) and four Chinese databases (CNKI, Wanfang, VIP and Sinomed) were searched. Randomized controlled trials (RCTs) and quasi-experimental studies investigating the effects of different frequencies of APE on lower limb hemodynamics published before July 2022 were included. The reference list was also searched. Seven studies (one RCTs and six quasi-experimental studies) were included in the systematic review and five studies (one RCTs and four quasi-experimental studies) were included in the NMA. The risk of bias was assessed using the Cochrane and Joanna Briggs Institute tools. The NMA was performed using the R software (version 4.2.1) and OpenBUGS (version 3.2.3). Results: The results of the NMA showed that a frequency of every 3e4 s the most effective in improving lower extremity hemodynamics (P ¼.85), followed by every 1e2 s (P ¼ .81), every 5e6 s (P¼.32) and less than every 10 s (P ¼.02). Subgroup analysis failed to find a difference between healthy participants and those with unilateral total hip arthroplasty or fracture (MD ¼ À0.23, 95% CI-5.92 to 4.61). Conclusions: Consequencely, for adult patients, with or without lower extremity disease, a frequency of every 3e4 s can be recommended as the optimal frequency of APE in clinical care practice.

MRI-guided Wire Localization Open Biopsy is Safe and Effective for Suspicious Cancer on Breast MRI

Wang, Hai-Yi,Zhao, Yu-Nian,Wu, Jian-Zhong,Wang, Zheng,Tang, Jing-Hai Asian Pacific Journal of Cancer Prevention 2015 Asian Pacific journal of cancer prevention Vol.16 No.5

Background: Magnetic resonance imaging of breast, reported to be a high sensitivity of 94% to 100%, is the most sensitive method for detection of breast cancer. The purpose of this study was to investigate our clinical experience in MRI-guided breast lesion wire localization in Chinese women. Materials and Methods: A total of 44 patients with 46 lesions undergoing MRI-guided breast lesion localization were prospectively entered into this study between November 2013 and September 2014. Samples were collected using a 1.5-T magnet with a special MR biopsy positioning frame device. We evaluated clinical lesion characteristics on pre-biopsy MRI, pathologic results, and dynamic curve type baseline analysis. Results: Of the total of 46 wire localization excision biopsied lesions carried out in 44 female patients, pathology revealed fourteen malignancies (14/46, 30.4%) and thirty-two benign lesions (32/46, 69.6%). All lesions were successfully localized followed by excision biopsy and assessed for morphologic features highly suggestive of malignancy according to the American College of Radiology Breast Imaging Reporting and Data System (BI-RADS) category of MRI (C4a=18, C4b=17, C4c=8,C5=3). Of 46 lesions, 37 were masses and 9 were non-mass enhancement lesions. Thirty-two lesions showed a continuous kinetics curve, 11 were plateau and 3 were washout. Conclusions: Our study showed success in MRI-guided breast lesion wire localization with a satisfactory cancer diagnosis rate of 30.4%. MRI-guided wire localization breast lesion open biopsy is a safe and effective tool for the workup of suspicious lesions seen on breast MRI alone without major complications. This may contribute to increasing the diagnosis rate of early breast cancer and improve the prognosis in Chinese women.

Land side truck traffic modeling at container terminals by a stationary two-class queuing strategy with switching

Jing Wang,Nathan N. Huynh,Edsel Pena 인하대학교 정석물류통상연구원 2022 JOURNAL OF INTERNATIONAL LOGISTICS AND TRADE Vol.20 No.3

Purpose – This paper evaluates an alternative queuing concept for marine container terminals that utilize a truck appointment system (TAS). Instead of having all lanes providing service to trucks with appointments, this study considers the case where walk-in lanes are provided to serve those trucks with no appointments or trucks with appointments but arrived late due to traffic congestion. Design/methodology/approach – To enable the analysis of the proposed alternative queuing strategy, the queuing system is shown mathematically to be stationary. Due to the complexity of the model, a discrete event simulation (DES) model is used to obtain the average waiting number of trucks per lane for both types of service lanes: TAS-lanes and walk-in lanes. Findings – The numerical experiment results indicated that the considered queuing strategy is most beneficial when the utilization of the TAS lanes is expected to be much higher than that of the walk-in lanes. Originality/value – The novelty of this study is that it examines the scenario where trucks with appointments switch to the walk-in lanes upon arrival if the TAS-lane server is occupied and the walk-in lane server is not occupied. This queuing strategy/policy could reduce the average waiting time of trucks at marine container terminals. Approximation equations are provided to assist practitioners calculate the average truck queue length and the average truck queuing time for this type of queuing system.

4-(Methylnitrosamino)-1-(3-pyridyl)-1-butanone Induces Retinoic Acid Receptor β Hypermethylation through DNA Methyltransferase 1 Accumulation in Esophageal Squamous Epithelial Cells

Wang, Jing,Zhao, Shu-Lei,Li, Yan,Meng, Mei,Qin, Cheng-Yong Asian Pacific Journal of Cancer Prevention 2012 Asian Pacific journal of cancer prevention Vol.13 No.5

Overexpression of DNA methyltransferase 1 (DNMT1) has been detected in many cancers. Tobacco exposure is known to induce genetic and epigenetic changes in the pathogenesis of malignancy. 4-(Methylnitrosamino)-1-(3-pyridyl)-1-butanone (NNK) is an important carcinogen present in tobacco smoke; however the detailed molecular mechanism of how NNK induces esophageal carcinogenesis is still unclear. We found that DNMT1 was overexpressed in ESCC tissues compared with paired non-cancerous tissues, the overexpression being correlated with smoking status and low expression of $RAR{\beta}$. The latter could be upregulated by NNK treatment in Het-1A cells, and the increased DNMT1 expression level reflected promoter hypermethylation and downregulation of retinoic acid receptor ${\beta}$($RAR{\beta}$). RNA interference mediated knockdown of DNMT1 resulted in promoter demethylation and upregulation of $RAR{\beta}$ in KYSE30 and TE-1 cells. 3-(4,5-Dimethyl-thiazol-2yl)-2,5-diphenyltetrazolium bromide (MTT) and flow cytometric analysis demonstrated that NNK treatment in Het-1A cells could enhance cell proliferation and inhibit cell apoptosis in a dose-dependent manner. In conclusion, DNMT1 overexpression is correlated with smoking status and low expression of $RAR{\beta}$ in esophageal SCC patients. NNK could induce $RAR{\beta}$ promoter hypermethylation through upregulation of DNMT1 in esophageal squamous epithelial cells, finally leading to enhancement of cell proliferation and inhibition of apoptosis.

Electro-hydraulic coupling jet printing technology based on Weissenberg effect under pulse voltage

Jing Wang,Xintao Wu,Wei Wang,Bing Xu,Yuanjie Fang,Jing Tang 대한기계학회 2021 JOURNAL OF MECHANICAL SCIENCE AND TECHNOLOGY Vol.35 No.6

In this paper, electro-hydraulic coupling jet printing technology based on the Weissenberg effect is put forward for the first time, and the pulse voltage is used to realize the on-demand printing. The experimental results show that the Weissenberg effect can inhibit the solution reflux and make the solution converge into a cone tip at the nozzle, which is more conducive to position printing precisely. By exploring the influence principles of process parameters on printing, it is found that droplet diameter is positively correlated with rotation speed and negatively correlated with voltage frequency. The printing frequency is negatively correlated with the rotation speed and positively correlated with the voltage frequency. However, the effect of voltage amplitude and duty ratio on the printing effect is not obvious in a certain range. It is found that the rotation speed of needle core within the range of 1800 r/min-7500 r/min can print out the ideal lattice structure, which proves the feasibility of this system to realize on-demand positioning printing. Finally, by using the printing equipment, the sacrificial layer structure of PDMS microfluidic chip was successfully made and the function of the microfluidic chip was realized, which further verified the practicability of the technical scheme proposed in this paper.

On-line Auxiliary Input Signal Design for Active Fault Detection and Isolation Based on Set-membership and Moving Window Techniques

Jing Wang,Junde Wang,Meng Zhou 제어·로봇·시스템학회 2019 International Journal of Control, Automation, and Vol.17 No.11

This paper presents an on-line auxiliary input signal design strategy based on set-membership and moving window techniques to deal with the problem of active fault detection and isolation. The goal of active fault detection and isolation is to design an auxiliary input signal, such that the nominal system output set and faulty systems output sets are separated each other after injecting the input signal. In this paper, the output sets are characterized by ellipsoids. First, an extended model of the system based on moving window technique is constructed, then an auxiliary input signal is calculated on-line based on the equivalent model. As the energy of the auxiliary input signal is restricted minimum to decrease the influence of the signal on the system, the design condition of active fault detection and isolation is transformed into an optimal problem. Furthermore, the fault is isolated by judging the actual system output belongs to which output ellipsoid of the faulty models, or determining the probability of the system output is in which faulty model when the output ellipsoids of faulty models are intersecting. Finally, numerical simulations illustrate the feasibility and effectiveness of the proposed approach.

Experimental Investigation of Ozone Decomposition in Diesel Particulate Filter Regeneration with Non-Thermal Plasma Technology

Wang Weikai,Cai Yi-xi,Shi Yunxi,Wang Jing,Zhao Nan,Ji Liang 한국자동차공학회 2021 International journal of automotive technology Vol.22 No.4

A non-thermal plasma (NTP) reactor was used to generate ozone for diesel particulate filter (DPF) regeneration. The kinetic mechanism of ozone thermal decomposition reaction is explored, and the effect of temperature on the change of ozone concentration is analyzed. The changes of the internal temperature and the concentration of regeneration products such as CO2 and CO during the regeneration under constant temperature and non-constant temperature conditions are then studied and the influence of different regeneration environments on the regeneration is analyzed in combination with the decomposition law of ozone. In the non-constant temperature condition, DPF surface temperature changes significantly with time. The results show that when using an NTP reactor to generate ozone, the activation energy of the decomposition reaction is 2.80755 × 104 J/mol and the law of thermal decomposition reaction can be described as 190.76 . /. During DPF regeneration, the overall regeneration rate and ozone utilization rate in the nonconstant temperature environment are higher than in the constant temperature environment and the temperature change (delta-T) peak rises with the increase of PM deposition. The regeneration with NTP under non-constant thermal condition is an effective way to improve the efficiency of DPF regeneration.

Color centers and dynamic emission of Eu2+ ion doped halosilicate Ca10Si6O21Cl2

Wang, Jing,Qin, Lin,Huang, Yanlin,Wei, Donglei,Seo, Hyo Jin Springer-Verlag 2014 APPLIED PHYSICS A MATERIALS SCIENCE AND PROCESSING Vol.115 No.4

Simulation Research on Jingdong Orders System Based on Flexsim

Wang Jing,Lilei 보안공학연구지원센터 2016 International Journal of Multimedia and Ubiquitous Vol.11 No.6

Order processing system is the beginning and core part of distribution center operations, and efficient order processing operations can not only improve customer satisfaction but also reduce costs. Therefore, the trade-off analysis between order processing difficulty and quick response time, improving the handling capacity and efficiency while reducing order processing costs are regarded as two big challenges for e-commercial enterprise. Based on queuing model as well as some data collected from Jingdong, a Chinese E-commerce giant, this paper constructed a 3D simulation model by using Flexsim, aiming at solving the bottleneck of the whole system of E-commerce order processing. According to the simulation, the author gives two order processing optimization solution, service stations optimization and queuing optimization to solve the two challenges.