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- 저자 : Jian Zhuge (검색결과 3 건)
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A Heuristic-Based Population Synthesis Method for Micro-Simulation in Transportation
Chengxiang Zhuge,Xia Li,Chia-An Ku,Jian Gao,Hui Zhang 대한토목학회 2017 KSCE Journal of Civil Engineering Vol.21 No.6
Population synthesis is extensively required by a number of micro-simulation models in transportation. A heuristic-based population synthesis method called Pop-H was proposed to overcome the following two limitations that received less attention. The first limitation is that one target marginal distribution can be well met by various sets of household weights that can be used to generate different sets of population and thus it is a problem that which set of household weights is the real one. Secondly, the population synthesis is commonly viewed as an optimization problem, and minimizing the Mean Absolute Percentage Error of control variables is generally used as the objective function. The Standard Deviation of control variables is also crucial in some cases, which, however receives scant attention. In response to these two limitations, the heuristic-based population synthesis method works in the following way: the Pop-H algorithm starts with the initial set of household weights derived from a sample data and calculates the final set of household weights by iteratively adjusting the initial set in a defined way with the objective function taking into account both Mean Absolute Percentage Error and Standard Deviation of control variables. Finally, the medium-sized city of Baoding, China was used as the case study. The sensitivity test was firstly done to examine four key parameters of the Pop-H algorithm, and then the algorithm was applied to create the population for the whole city.
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Interaction between Barium Oxide and Carbon Film in BaO/C/Mo System under High Temperature
Yue Hui Lu,Xue Mei Wu,Lan Jian Zhuge,Xiang Huai Liu 한국물리학회 2005 THE JOURNAL OF THE KOREAN PHYSICAL SOCIETY Vol.46 No.1
Carbon ¯lm, used as the coating of the Mo grid in pulsed-controlled grid traveling wave tubes, can eectively suppress electron emission from the Mo grid contaminated by the emission material of the hot cathode, i.e. BaO or Ba, so that the lifetime of the tubes can be prolonged signi¯cantly but the reasons for it have not been well understood. To study the eect of it on the BaO/Mo system under high temperature, carbon ¯lms were prepared on Mo substrate at room temperature by a dual-ion-beam sputtering deposition system, and post-annealing was conducted to know their microstructure under high temperature. In our experiments, BaO layers were coated on Mo and carbon-coated Mo substrates by the chemical method to compare with each other, and the prepared BaO/Mo and BaO/C/Mo samples were annealed at two dierent temperatures of 973 K and 1223 K in order to investigate the interaction between barium oxide and carbon ¯lm under high temperature. The results show that the BaO/C/Mo changes into C/Mo after the exhaustion of BaO at 1223 K, nevertheless, This does not happen at 973 K. In this paper, the mechanism whereby the addition of carbon ¯lm can suppress the grid emission under its operating condi-tion is discussed according to the experimental results and the calculation of the reaction free energy.
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Cheng Zhang,Hong Zong,Bin Zhuge,Xinyao Lu,Huiying Fang,Jiali Zhu,Jian Zhuge 한국생물공학회 2016 Biotechnology and Bioprocess Engineering Vol.21 No.1
The regeneration of Candida glycerinogenes protoplasts is a major step following genetic manipulations such as fusion and DNA-mediated transformation. An investigation of protoplast formation and cytological examination was used to gain further insight into the loss of protoplast viability in osmotically stabilized support media. Protoplasts with the highest regeneration frequency (98.6% protoplasts/mL) were isolated, using lysozyme dissolved in 1M sorbitol osmoticum. The commercial enzyme preparations, osmotic stabilisers, and growth phase were effective in raising the protoplast yield. Sodium chloride was effective for protoplast preparation; however, sugars and sugar alcohols were better for protoplast regeneration. Sorbitol at a concentration of 1 M was used in regeneration agar for further studies. Regeneration of colonies from protoplasts was maximal (11 ~ 15%) when protoplasts were incorporated in cooled agar containing 0.5% glucose, supplemented with 1M sorbitol as osmotic stabilizer. C. glycerinogenes strain was highly sensitive to zeocin, so transformation of protoplasts and PEG-mediated was achieved with an improved transformation system, using plasmid pURGAP-gfp containing zeocin gene driven by a PCgGAP promoter from C. glycerinogenes to express gfp gene and be transformed into the 5.8S rDNA site of C. glycerinogenes in order to test the system for studying the yeast osmoregulation. We developed an efficient method for transformation of C. glycerinogenes, and parameters involved in transformation efficiency were optimized. Expressions of gfp at different levels were conducted under osmotic stress containing NaCl, KCl, sorbitol or glycerol for the recombinant strains. These improved procedures for protoplast isolation, regeneration and transformation proved to be useful applications in genetic studies for other Candida species and industrial yeast.
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