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      • KCI등재SCOPUS

        국내 시판 액상요구르트의 품질특성

        이지현,박혜영,원주인,박혜인,최인덕,이석기,박지영,조동화,전용희,오세관,한상익,최혜선,Lee, Ji Hyun,Park, Hye Young,Won, Ju In,Park, Hye In,Choi, In Duck,Lee, Seuk Ki,Park, Ji Young,Joe, Dong Hwa,Jeon, Yong Hee,Oh, Sea Kwan,Han, Sang Ik,Ch 한국식품저장유통학회 2017 한국식품저장유통학회지 Vol.24 No.6

        Yogurt is produced by fermentation of milk using bacteria known as "yogurt cultures". Most of these bacteria are probiotics such as Lactobacillus delbrueckii subsp. bulgaricus, Lb. rhamnosus, Streptococcus thermophilus, and Bifidobacterium. The domestic fermented milk market is increasing, and about 30 companies are producing yogurt. The purpose of this study was to analyze the quality characteristics of domestic commercial liquid yogurt. Total 30types of commercial yogurts were sampled and their physicochemicial properties, including pH, sugar content, acidity, viscosity, and microbial characteristics of lactic acid bacteria counts, were measured. Commercial liquid type yogurt showed a pH of 4.5, sugar content of 7.4-21.2%, total acid content of 0.4-0.9%, and viscosity of 0.1-250 cP. In terms of microbial populations, lactic acid bacteria counts were 7.2-11.3 log CFU/mL and anaerobic lactic acid bacteria counts were 8.0-11.5 log CFU/mL. The quality characteristics differed depending on the constituents of the sample and the microorganisms used. These results are related to the quality characteristics of yogurts and are useful for identifying new trends in the domestic fermented milk industry. 요구르트는 유산균을 이용하여 제조한 대표적인 유발효식품이다. 국내 유통되고 있는 요구르트는 약 30개의 업체에서 생산되고 있으며, 최근 당 저감화 정책 실시로 저당제품이 출시되고 있다. 본 연구는 국내 시판되고 있는 액상타입의 요구르트를 수집하여 제품의 일반적인 특성을 조사하였고, pH, 산도, 당도, 점도, 호기성 혐기성 유산균수를 측정하여 기초자료로 사용하고자 하였다. 탈지분유(97%) 및 원유(57%)가 주된 원료로 높은 빈도로 사용되고 있으며, 발효스타터는 요구르트에 100% 이용되고 있으며 주로 수입균주가 이용되고 있다. 당은 sucrose, oligosaccharide, xylitol이 첨가되었고, 그 외 구연산, vitamin C, gum류 등이 요구르트의 기능성과 물성 등을 개선하고자 하는 부원료로 사용되고 있는 것을 확인하였다. 30종의 요구르트의 품질특성을 분석한 결과 pH는 3.6-4.6, 당도는 7.4-21.2%, 총산도는 0.4-0.9%, 호기성 유산균수 7.2-11.3 log CFU/mL, 혐기성 유산균수는 8.0-11.5 log CFU/mL로 분석되었다. 본 연구에서 도출된 시판 액상타입 요구르트의 품질분석 결과는 다양한 소재를 접목한 요구르트 개발의 품질규격설정의 기초자료로 유용 할 것으로 사료된다.

      • KCI등재

        큰느타리버섯의 신선도 유지기간 연장을 위한 CO<sub>2</sub> 처리 효과

        이지현,최지원,홍윤표,최현진,김지강,Lee, Ji-Hyun,Choi, Ji-Weon,Hong, Yoon-Pyo,Choi, Hyun-Jin,Kim, Ji-Gang 한국버섯학회 2014 한국버섯학회지 Vol.12 No.4

        큰느타리버섯은 장거리 해상운송 후 갈변 및 조직 물러짐에 의한 품질저하가 클레임의 원인이 되는데, 수출 중 신선도 유지를 위한 전처리 기술을 개발하고자 $CO_2$ 처리의 효과를 검증하였다. 버섯의 수확후 $5^{\circ}C$에서 30, 50% 농도의 $CO_2$를 3시간 처리하고 관행적인 방법으로 필름포장한 다음 $20^{\circ}C$와 $5^{\circ}C$에 저장하며 무처리와의 품질을 비교분석하였다. $CO_2$ 처리구는 무처리에 비해 저장 중 조직 경도와 색도(Hunter 'L', 'b')를 유지하였고, 관능평가에서도 품질이 급격히 저하되는 저온저장 3주차에 조직물러짐 정도와 갈변지수가 낮아 신선도 유지에 효과가 있었다. $CO_2$ 처리 농도 중 30% 처리구가 50%에 비해 상품성이 높았으며 무처리에 비해서는 $5^{\circ}C$에서 약 1주일간 신선도 유지기간이 연장되었다. 따라서 큰느타리버섯의 장거리 해상운송 중 품질유지를 위한 전처리 방법으로 $CO_2$ 처리가 효과가 있는 것으로 판단되며 현장적용을 위해 효과가 있는 $CO_2$ 처리농도의 범위 및 장해를 유발하는 한계농도의 설정과 처리시간 등 추가적인 실험이 요구된다. King oyster mushrooms(Pleurotus eryngii) are the second biggest mushroom for exporting in Korea but their browning and soft rot is the main factors of claim during long distance transportation. Fresh king oyster mushrooms were treated with $CO_2$ at 30, 50% for 3 hours at $5^{\circ}C$ prior to storage at $20^{\circ}C$ and $5^{\circ}C$. There was no difference on respiration rate after $CO_2$ treatment. However exposure to $CO_2$ for 3h prior to MA packing maintained the firmness and delayed color(hunter L and b value) change of mushrooms during storage. Especially an incubation in high $CO_2$ at 30% significantly reduced soft rot and browning symptoms resulting in one week extension of shelf-life during storage at $5^{\circ}C$ compared to control and 50% $CO_2$ treatment.

      • SCOPUSKCI등재

        수종 Flavonoid의 항허피스바이러스효과

        이지현,김영소,이종길,이혁구,한성순,Lee, Ji-Hyun,Kim, Young-So,Lee, Chong-Kil,Lee, Hyuk-Koo,Han, Seong-Sun 한국생약학회 1999 생약학회지 Vol.30 No.1

        To search for less toxic antiherpetic agents, the inhibitory effects of twelve kinds of flavonoids including chrysin, quercetin, quercitrin, rutin, fisetin, gossypin, kaempferol, morin, naringenin, naringin, hesperetin and hesperidin on the plaque formation of herpes simplex virus type 1 (HSV-1) and type 2 (HSV-2) in Vero cells were examined by plaque reduction assay in vitro. Some flavonoids tested in this study showed potent antiherpetic activity, reducing intracellular replication of herpes simplex viruses when Vero cell monolayers were infected and subsequently cultured in medium containing flavonoids. Naringenin showed the most potent antiviral activity against HSV-1 with selectivity index (SI) of 19.1 and hesperetin showed the most potent antiviral activity against HSV-2 with SI of 9.8. These results suggest that some flavonoids may be a potential therapeutic agent for the treatment and prevention of herpes simplex virus infections.

      • SCOPUSKCI등재

        천연 Hesperetin의 항허피스바이러스작용과 Acyclovir 및 Vidarabine과의 병용효과

        이지현,어성국,김영소,이종길,한성순,Lee, Ji-Hyun,Eo, Seong-Kug,Kim, Young-So,Lee, Chong-Kil,Han, Seong-Sun 한국생약학회 1999 생약학회지 Vol.30 No.1

        To search for less toxic antiherpetic agents, the inhibitory effects of natural hesperetin on the plaque formation of herpes simplex virus type 1 (HSV-1) and type 2 (HSV-2) in Vero cells were examined by the plaque reduction assay in vitro. Hesperetin inhibited plaque formations of HSV-1 and HSV-2 in a dose dependent manner. It also exhibited more potent antiherpetic activity on HSV-2 with selectivity index (SI) of 9.8 than on HSV-1 with SI of 8.4. The combined antiherpetic effects of hesperetin with nucleoside antiherpetic agents, acyclovir and vidarabine, were examined on the multiplication of these two strains of herpesviruses in Vero cells by the combination assay. The results of combination assay were evaluated by the combination index (CI) that was calculated by the multiple drug effect analysis. The combinations of hesperetin with acyclovir on HSV-1 and HSV-2 showed synergistic effects with CI values of $0.29{\sim}0.73$ for 50%, 70%, 90% effective levels and those with vidarabine showed partially synergistic or additive effects with CI values of $0.83{\sim}1.33$.

      • KCI등재후보

        살균수 발생장치에 의해 생성된 양치용액이 치주질환에 미치는 효과

        이지현,윤정호,최성호,조규성,Lee, Ji-Hyun,Yun, Jeong-Ho,Choi, Seong-Ho,Cho, Kyoo-Sung 대한치주과학회 2004 Journal of Periodontal & Implant Science Vol.34 No.3

        The aim of this study was to evaluate the clinical efficacy of mouthrinses prepared by sterilized water-generating $device(Purister^{(R)})$ on the control of gingivitis and incipient periodontitis when it was used as a adjunctives to the mechanical plaque control. 40 healthy patients with gingivitis or incipient periodontitis were divided into two groups. Patients in the experimental group use mouthrinses prepared by sterilized water-generating $device(Purister^{(R)})$ after tooth brushing while patients in the control group do only tooth brushing for plaque control. All patients received scaling and tooth brushing instruction. 1 week after scaling was set as baseline. Probing pocket depth, clinical attachment level, and bleeding on probing were scored at baseline, 4 weeks. Gingival index and plaque index were scored at baseline, 2 weeks, and 4 weeks. The results were as follows: 1. In the experimental group, gingival index, plaque index, probing pocket depth, and clinical attachment level showed statistically significant decrease, but in the control group, significant increase(p<0.05). 2. There was no significant difference between the experimental and the control group in bleeding on probing, but significant decrease in the experimental group and Significant increase in the control group In a time-dependent manner(p <0.05). From these results, it can be concluded that regular use of mouthrinses prepared by sterilized water-generating $device(Purister^{(R)})$ as adjunctives of mechanical plaque control, may be effective to prevent and treat gingivitis and incipient periodontitis.

      • KCI등재

        청석면 분석 정도관리용 표준시료 개발 및 평가

        이지현,김은영,노수진,박용진,정지연,Lee, Ji-Hyun,Kim, Eun-Young,Noh, Su-Jin,Park, Yong-Jin,Jeong, Jee-Yeon 한국환경보건학회 2011 한국환경보건학회지 Vol.37 No.1

        Crocidolite quality control (QC) sampling created by the wet generation method has been validated by validation tests such as the accuracy, precision, and storage tests. For this study we designed and developed a manufacturing apparatus and standard operating procedure for making these QC samples. The most important step in the procedure of making QC samples was the stage eliminating static electricity in asbestos fibers. This static electricity hampers the fibers clog functioning. In accuracy and precision tests by phase contrast microscopy analysis, the difference between the reference values and the studied values was at maximum 17.8%. This satisfies the AIHA proficiency analytical test criteria for asbestos. We could confirm the nearly even distribution of crocidolite fibers on the membrane filter. Also, there was no loss of fibers in the storage test after the one month.

      • KCI등재

        스트레스성 고혈압 환자에서 고혈압 약물 복용 없는 복합한의치료만의 강압 효과 - 증례보고

        이지현,노민영,윤석영,전형선,한양희,임정태,Lee, Ji-hyun,Roh, Min-yeong,Yoon, Seok-yeong,Jun, Hyung-sun,Han, Yang-hee,Leem, Jung-tae 대한한방내과학회 2021 大韓韓方內科學會誌 Vol.42 No.5

        Objectives: This study considered the effectiveness of integrative Korean medical treatment for stress-induced hypertensive patients without the use of conventional medication. Methods: A 62-year-old female with stress-induced hypertension (HTN) was hospitalized for 33 days. Her pattern identification was ascendant hyperactivity of liver yang (Ganyangsanghang)-type HTN. Herbal medicine and acupuncture were used for the treatment: Cheonggansoyo-san for 33 days and Chunwangbosim-dan for 15 days, together with acupuncture for 20 minutes twice a day. Blood pressure was checked daily during hospitalization with a digital sphygmomanometer in the brachial artery. Results: After 33 days of treatment, blood pressure decreased. The Handicap Inventory (DHI) scale and Pittsburg Sleep Quality Index (PSQI) scores both decreased. The patient also reported fewer complaints. A five-month follow-up after discharge, with no further treatment, confirmed stable blood pressure. Symptom improvements continued with no significant side effects. Conclusions: This study indicates that Korean medical treatment is effective for stress-induced hypertensive patients.

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