Polymorphisms in cancer-related pathway genes and lung cancer

Lee, Shin Yup,Kang, Hyo-Gyoung,Choi, Jin Eun,Jung, Deuk Kju,Lee, Won Kee,Lee, Hyun Chul,Lee, So Yeon,Yoo, Seung Soo,Lee, Jaehee,Seok, Yangki,Lee, Eung Bae,Cha, Seung Ick,Cho, Sukki,Kim, Chang Ho,Lee, European Respiratory Society 2016 The European respiratory journal Vol.48 No.4

<P>We evaluated the associations between potentially functional variants in a comprehensive list of cancer-related genes and lung cancer in a Korean population.</P><P>A total of 1969 potentially functional single nucleotide polymorphisms (SNPs) of 1151 genes involved in carcinogenesis were evaluated using an Affymetrix custom-made GeneChip in 610 nonsmall cell lung cancer patients and 610 healthy controls. A replication study was conducted in an independent set of 490 cases and 486 controls. 68 SNPs were significantly associated with lung cancer in the discovery set and tested for replication.</P><P>Among the 68 SNPs, three SNPs (corepressor interacting with RBPJ 1 (<I>CIR1</I>) rs13009079T>C, ribonucleotide reductase M1 (<I>RRM1</I>) rs1465952T>C and solute carrier family 38, member 4 (<I>SLC38A4</I>) rs2429467C>T) consistantly showed significant associations with lung cancer in the replication study. In combined analysis, adjusted odds ratio for <I>CIR1</I> rs13009079T>C, <I>RRM1</I> rs1465952T>C and <I>SLC38A4</I> rs2429467C>T were 0.69, 0.71 and 0.73, respectively (p=4×10<SUP>−5</SUP>, 0.01 and 0.001, respectively) under the dominant model. The relative mRNA expression level of <I>CIR1</I> was significantly associated with rs13009079T>C genotypes in normal lung tissues (ptrend=0.03).</P><P>These results suggest that the three SNPs, particularly <I>CIR1</I> rs13009079T>C, may play a role in the pathogenesis of lung cancer.</P>

Are Spinal GABAergic Elements Related to the Manifestation of Neuropathic Pain in Rat?

Jaehee Lee,Seung Keun Back,Eun Jeong Lim,Gyu Chong Cho,Myung Ah Kim,Hee Jin Kim,Min Hee Lee,Heung Sik Na 대한생리학회-대한약리학회 2010 The Korean Journal of Physiology & Pharmacology Vol.14 No.2

Impairment in spinal inhibition caused by quantitative alteration of GABAergic elements following peripheral nerve injury has been postulated to mediate neuropathic pain. In the present study, we tested whether neuropathic pain could be induced or reversed by pharmacologically modulating spinal GABAergic activity, and whether quantitative alteration of spinal GABAergic elements after peripheral nerve injury was related to the impairment of GABAergic inhibition or neuropathic pain. To these aims, we first analyzed the pain behaviors following the spinal administration of GABA antagonists (1Ռg bicuculline/rat and 5Ռg phaclofen/rat), agonists (1Ռg muscimol/rat and 0.5Ռg baclofen/rat) or GABA transporter (GAT) inhibitors (20Ռg NNC-711/rat and 1Ռg SNAP-5114/rat) into naïve or neuropathic animals. Then, using Western blotting, PCR or immunohistochemistry, we compared the quantities of spinal GABA, its synthesizing enzymes (GAD65, 67) and its receptors (GABA_A and GABA_B) and transporters (GAT-1, and -3) between two groups of rats with different severity of neuropathic pain following partial injury of tail-innervating nerves; the allodynic and non-allodynic groups. Intrathecal administration of GABA antagonists markedly lowered tail-withdrawal threshold in naïve animals, and GABA agonists or GAT inhibitors significantly attenuated neuropathic pain in nerve-injured animals. However, any quantitative changes in spinal GABAergic elements were not observed in both the allodynic and non-allodynic groups. These results suggest that although the impairment in spinal GABAergic inhibition may play a role in mediation of neuropathic pain, it is not accomplished by the quantitative change in spinal elements for GABAergic inhibition and therefore these elements are not related to the generation of neuropathic pain following peripheral nerve injury.

Comparison of the Performance of Chamber and Bag Digesters for Solid State Anaerobic Digestion of Separated Solid Fraction of Swine Manure

Lee, Jaehee,Lee, Seunghun,Kim, Eunjong,Jo, Hyunsoo,Ahn, Heekwon 한국토양비료학회 2015 한국토양비료학회지 Vol.48 No.2

The performance of chamber and bag digesters for solid state anaerobic digestion (SS-AD) of separated solid fraction of swine manure was investigated using lab-scale digester (4,460 mL total volume and 1,800 mL of effective volume) operating at $37^{\circ}C$ for 63 days. The performance of two different digester types was evaluated in terms of the kinetic constants of methane production obtained from the Gompertz and Gaussian equations. Methane production potential of chamber and bag digester was 202 and $218N{\cdot}mL$ $CH_4/g$ VS. Time to produce 95% methane production potential (T95) and calculated effective anaerobic digestion time were 55.5 days and 41.8 days for chamber digester and 52.8 days and 43.5 days for bag digester, respectively. Our results reveal that the performance was not significantly different between chamber and bag digester.

A Functional Polymorphism in the <i>CHRNA3</i> Gene and Risk of Chronic Obstructive Pulmonary Disease in a Korean Population

Lee, Jae Yeon,Yoo, Seung Soo,Kang, Hyo-Gyoung,Jin, Guang,Bae, Eun Young,Choi, Yi Young,Choi, Jin Eun,Jeon, Hyo-Sung,Lee, Jaehee,Lee, Shin Yup,Cha, Seung-Ick,Kim, Chang Ho,Park, Jae Yong The Korean Academy of Medical Sciences 2012 JOURNAL OF KOREAN MEDICAL SCIENCE Vol.27 No.12

<P>A genome-wide association study has identified the 15q25 region as being associated with the risk of chronic obstructive pulmonary disease (COPD) in Caucasians. This study intended as a confirmatory assessment of this association in a Korean population. The rs6495309C > T polymorphism in the promoter of nicotinic acetylcholine receptor alpha subunit 3 (<I>CHRNA3</I>) gene was investigated in a case-control study that consisted of 406 patients with COPD and 394 healthy control subjects. The rs6495309 CT or TT genotype was associated with a significantly decreased risk of COPD when compared to the rs6495309 CC genotype (adjusted odds ratio = 0.69, 95% confidence interval = 0.50-0.95, <I>P</I> = 0.023). The effect of the rs6495309C > T on the risk of COPD was more evident in moderate to very severe COPD than in mild COPD under a dominant model for the variant T allele (<I>P</I> = 0.024 for homogeneity). The <I>CHRNA3</I> rs6495309C > T polymorphism on chromosome 15q25 is associated with the risk of COPD in a Korean population.</P>

Operation Scheduling Method for Battery Energy Storage System in a Wind Farm Considering Wind Power Forecast Error

Jaehee Lee(이재희),SeoEun Son(손서은),Min Hyug Park(박민혁) 한국신재생에너지학회 2014 한국신재생에너지학회 학술대회논문집 Vol.2014 No.05

Effect of Tartary Buckwheat Extract on Hangover Releif

Jaehee LEE,Kyungdae KIM,Kiyeon LEE,Eunha JANG,Jingwan HAM 한국생물공학회 2022 한국생물공학회 학술대회 Vol.2022 No.9

Detection of Deep Vein Thrombosis by Follow-up Indirect Computed Tomography Venography after Pulmonary Embolism

Lee, Hye Jin,Cha, Seung-Ick,Shin, Kyung-Min,Lim, Jae-Kwang,Yoo, Seung-Soo,Lee, Shin-Yup,Lee, Jaehee,Kim, Chang-Ho,Park, Jae-Yong The Korean Academy of Tuberculosis and Respiratory 2018 Tuberculosis and Respiratory Diseases Vol.81 No.1

Background: Information regarding the incidence and risk factors for deep vein thrombosis (DVT) detected by follow-up computed tomographic (CT) venography after pulmonary embolism (PE) is sparse. The aim of the present study was to identify the predictors of DVT in follow-up CT images, and to elucidate their clinical significance. Methods: Patients with PE were classified into the following three cohorts based on the time of indirect CT venography follow-up: within 1 month, 1 to 3 months, and 3 to 9 months after the initial CT scan. Each cohort was subdivided into patients with or without DVT detected by follow-up CT. Clinical variables were compared between the two groups. Results: Follow-up CT revealed DVT in 61% of patients with PE within 1 month, in 15% of patients with PE at 1 to 3 months, and in 9% of patients with PE at 3 to 9 months after the initial CT scan. Right ventricular (RV) dilation on the initial CT (odds ratio [OR], 8.30; 95% confidence interval [CI], 1.89-36.40; p=0.005) and proximal DVT at the initial presentation (OR, 6.93; 95% CI, 1.90-25.20; p=0.003) were found to independently predict DVT in follow-up CT images within 1 month, proximal DVT at the initial presentation was found to independently predict DVT in follow-up CT images at 1 to 3 months (OR, 6.69; 95% CI, 1.53-29.23; p=0.012), and central PE was found to independently predict DVT in follow-up CT images at 3 to 9 months (OR, 4.25; 95% CI, 1.22-4.83; p=0.023) after the initial CT scan. Furthermore, the detection of DVT by follow-up CT independently predicted the recurrence of venous thromboembolism (VTE) (OR, 4.67; 95% CI, 2.24-9.74; p<0.001). Conclusion: Three months after PE, DVT was not detected by follow-up CT in most patients with PE. RV dilation on the initial CT, central PE, and proximal DVT at the initial presentation were found to predict DVT on follow-up CT, which might predict VTE recurrence.

Genexol-PM/carboplatin versus paclitaxel/carboplatin with or without bevacizumab in advanced high-grade serous ovarian cancer: interim analysis of a prospective cohort study with historical control

( Jaehee Mun ),( Seokyung Kim ),( Eun Ji Lee ),( Soo Jin Park ),( Joo-hyuk Son ),( Maria Lee ),( Tae-wook Kong ),( Hee Seung Kim ),( Jiheum Paek ),( Hyun Hoon Chung ),( Suk-joon Chang ),( Jae Weon Kim 대한산부인과학회 2020 대한산부인과학회 학술대회 Vol.106 No.-

Objective: Cremophor-free polymeric micelle formulation of paclitaxel (Genexol-PM) plus carboplatin (PM/C) has been reported to have a non-inferior effect with tolerable toxicities when compared to the standard paclitaxel plus carboplatin (P/C) in ovarian cancer. However, there is still a lack of evidence on the effect of an increased dose of paclitaxel in PM/C, especially, when compared to P/C and paclitaxel plus carboplatin with bevacizumab (P/C/B) in advanced high-grade serous ovarian cancer (HGSO). Thus, we performed a prospective cohort study on PM/C and compared the effect of PM/C with historical control of P/C and P/C/B in HGSO. Methods: We performed a prospective cohort study between October 2015 and June 2019. Patients aged 20 or more years with FIGO stage III-IV HGSO who received PM (260 mg/m²)/C (AUC 5) after primary debulking surgery (PDS) were enrolled. We collected clinico-pathologic data from a retrospective cohort when P (175 mg/m²)/C (AUC 5) or P (175 mg/m²)/C (AUC 5)/B (15 mg/kg) were used as adjuvant treatment after PDS during the same period. Results: A total of 104 patients were enrolled, and 17, 28, and 59 received P/C, P/C/B and, PM/C respectively (Table 1). Complete response was significantly highest in PM/C (29.4 vs. 39.3 vs. 61%, P=0.030; Table 1). Progression-free survival was longest in PM/C (Figure 1) and multivariate analysis showed that gross residual tumor after PDS and P/C were poor prognostic factors (adjusted hazard ratios, 2.415 and 2.751; 95% confidence intervals, 1.172-4.976 and 1.214-6.236; Table 2). Even after adjustment of the patient pool to those with no gross residual tumor after PDS, multivariate analysis still showed that P/C lowered the survival curve (adjusted hazard ratio, 3.342; 95% confidence interval, 1.143-9.777; Table 2). Conclusion: This interim analysis showed that PM/C had a comparable effect to P/C/B for stage III-IV HGSO patients who received optimal cytoreduction during PDS.

Clinical Utility of CT-Based Bronchial Aspirate TB-PCR for the Rapid Diagnosis of Pleural Tuberculosis

Lee, Jaehee,Lee, So Yeon,Choi, Keum Ju,Lim, Jae Kwang,Yoo, Seung Soo,Lee, Shin Yup,Cha, Seung Ick,Park, Jae Yong,Kim, Chang Ho The Korean Academy of Tuberculosis and Respiratory 2013 Tuberculosis and Respiratory Diseases Vol.75 No.4

Background: Thoracoscopic pleural biopsy is often required for rapid and confirmative diagnosis in patients with suspected pleural tuberculosis (PL-TB). However, this method is more invasive and costly than its alternatives. Therefore, we evaluated the clinical utility of the chest computed tomography (CT)-based bronchial aspirate (BA) TB-polymerase chain reaction (PCR) test in such patients. Methods: Bronchoscopic evaluation was performed in 54 patients with presumptive PL-TB through diagnostic thoracentesis but without a positive result of sputum acid-fast bacilli (AFB) smear, pleural fluid AFB smear, or pleural fluid TB-PCR test. Diagnostic yields of BA were evaluated according to the characteristics of parenchymal lesions on chest CT. Results: Chest radiograph and CT revealed parenchymal lesions in 25 (46%) and 40 (74%) of 54 patients, respectively. In cases with an absence of parenchymal lesions on chest CT, the bronchoscopic approach had no diagnostic benefit. BA TB-PCR test was positive in 21 out of 22 (95%) patients with early-positive results. Among BA results from 20 (37%) patients with patchy consolidative CT findings, eight (40%) were AFB smear-positive, 18 (90%) were TB-PCR-positive, and 19 (95%) were culture-positive. Conclusion: The BA TB-PCR test seems to be a satisfactory diagnostic modality in patients with suspected PL-TB and patchy consolidative CT findings. For rapid and confirmative diagnosis in these patients, the bronchoscopic approach with TB-PCR may be preferable to the thoracoscopy.

Development of Foodwaste Drying Technology using Natural Gas Heat Source

( Jaehee Lee ),( Jangwook Choi ),( Shinmook Choi ) 한국폐기물자원순환학회(구 한국폐기물학회) 2019 ISSE 초록집 Vol.2019 No.-

Since 2013, the whole foodwaste 5 million ton per annum had to be processed inland of the South Korea without any dumping in ocean whatsoever, and as a result of it the foodwaste processing and recycling cost has been increasing constantly which the cost is 1.25 trillion won (250,000 Won per ton, approx. USD1.13 billion per annum and USD225/ton-foodwaste) per annum. This is a tremendous expense, but most importantly illegal disposal such as illegally burying in land somewhere pollutes the environment, which is now recognized as a national problem to be resolved. It is each municipality’s to properly process and recycle the foodwaste. There is a substantial debate where Ministry of Environment tempts to expand the disposal bin equipped with RFID (Radio- Frequency Identification) as well as disposer (Discharge foodwaste to the sewage line after grinding), which the municipalities have very much low recycling rates. In fact, the disposer creates greater problems such as pipe blockage and huge overload on the final sewage treatment plant. The best solution for the problems abovementioned is the foodwaste dryer to be installed wherever the foodwaste is produced, then the problems can be easily resolved. On the other hand, the electricity cost for the electric heat source dryer is a burden, and GHG (Greenhouse gas) emission is burden too. It is the importance and the necessity to develop the dryer in other to use the gas heat source of dryer that is a clean energy and reducing the GHG emission at the same time1). In order to secure the reliability of the drying performance of the dryer with the vapor closed-loop recirculation drying process, the automatic cooling and termination program using the vapor temperature difference was applied. As a result, compared with the electric heat source dryer, the operation cost of the gas heat source dryer was reduced by 41%, and the GHG (Greenhouse gas) emission was reduced by 57%. In addition, the performance of the gas heat source dryer (SMER, Specific Moisture Evaporation Rate, 0.88 kg-water/Mcal) improved to 75.2% of the electric heat source dryer (SMER, 1.17 kgwater/ Mcal).