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        Effect of Mobile Phase Additives on Resolution of Some Nucleic Compounds in High Performance Liquid Chromatography

        Jin, Chun-Hua,Koo, Yoon-Mo,Choi, Dae-Ki,Row, Kyung-Ho Korean Society for Biotechnology and Bioengineerin 2007 Biotechnology and Bioprocess Engineering Vol.12 No.5

        Here we investigate the chromatographic behavior, with reversed-phase high performance liquid chromatography (RP-HPLC), of nucleic compounds (nucleobases, nucleosides, and nucleotides) on a $C_{18}$ column in several different mobile phase additives, including 1-butyl-3-methylimidazolium tetrafuloroborate $([BMlm][BF_4])$, 1-ethyl-3-methylimidazolium methylsulfate ([EMlm][MS]) ionic liquids, ammonium formate, and potassium phosphate. The effect of the alkyl group length, the imidazolium ring, and the ionic liquid's counterions on retention and resolution of the samples were tested. The results show the potential application of a used buffer system, ion pairing system, and ionic liquid as mobile phase additives in liquid chromatography resolution of nucleic compounds.

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        Effect of Concentration of Ionic Liquids on Resolution of Nucleotides in Reversed-phase Liquid Chromatography

        Jin Chun Hua,Yulia Polyakova,노경호 대한화학회 2007 Bulletin of the Korean Chemical Society Vol.28 No.4

        The chromatographic behaviors of nucleotides (inosine 5'-monophosphate, uridine 5'-monophosphate, guanosine 5'-monophosphate, and thymine monophosphate disodium salts) on a C18 column were studied with different types of ionic liquids (ILs) as additives for the mobile phase in reversed-phase liquid chromatography (RPLC). Three ILs, 1-butyl-3-methylimidazolium tetrafluoroborate ([BMIm][BF4]), 1-ethyl-3-methylimidazolium tetrafluoroborate ([EMIm][BF4]), and 1-ethyl-3-methylimidazolium methylsulfate ([EMIm][MS]), were used. Eluents were composed of water and methanol (90/10%, vol) with the addition of 0.5-13.0 mM of ILs. The effects of the concentration of ILs on retention and separation were investigated and discussed. The results showed that the addition of ILs affects the retention and resolution of the tested compounds. Use of 13.0 mM of [BMIm][BF4] as the eluent modifier resulted in a baseline separation of nucleotides without requiring gradient elution. This study demonstrates that ILs can be potentially applied as a mobile phase modifier in RPLC.

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        ‘교육공동체’에 대한 최근 연구동향에 대한 비판적 고찰 - 언어 네트워크 분석방법을 활용하여

        이유나,JINCHUNHUA,이상수 교육종합연구원 2016 교육종합연구 Vol.14 No.2

        The purpose of this study was to explore the future direction of research for the educational community by a critically review of recent research for 15 years related to the educational community through semantic network analysis. Main results were as follows. First, it claimed a total of 44 high frequency in studies related to the educational community for 15 years, the main word was mentioned ‘teacher', ‘community', ‘participation', ‘relationship between members' ‘caring ', ‘trust ', ‘collaboration ', ‘the teacher association’, etc. Second, the frequency of the word 'autonomy' is low but could show a significantly higher impact to identify the school as a community approach. Third, a educational community, a teacher, and a school community focused on consisting of key words were derived in cluster network analysis of educational community. It shows that not deviate from concepts or features, or components of the educational community have said in previous studies abroad. Also, it emphasized the role of the teacher becomes more prominent than the other members, or sometimes it limited to one theory, the research was done mainly to see education practice in Korea. Fourth, this study separated and analysed articles published in the former period(2001-2007) and the post period(2008-2015). Through the analysis, understanding the community as a way of overcoming conflict studies education consisted mainly of interest in voluntary participation of teachers in the former period but as well teacher as members of the community, and research focused on the relationship between the members through a sense of community, commitment and collaboration based on the shared vision and goals are identified as the main accomplished in the post period. According to the results of this study, previous studies of the educational community can have access to have occurred mainly applied to Korea around the theory of foreign studies, for innovation to education through a community approach It suggests that require a substantial theoretical and practical research, its practical strategy of South Korean education community, and focus on competencies development according to the Korean community education appropriate to the situation and context in South Korea.

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