Decreased Expression of LKB1 Correlates with Poor Prognosis in Hepatocellular Carcinoma Patients Undergoing Hepatectomy

Huang, Yue-Han,Chen, Zhen-Kun,Huang, Ka-Te,Li, Peng,He, Bin,Guo, Xu,Zhong, Jun-Qiao,Zhang, Qi-Yu,Shi, Hong-Qi,Song, Qi-Tong,Yu, Zheng-Ping,Shan, Yun-Feng Asian Pacific Journal of Cancer Prevention 2013 Asian Pacific journal of cancer prevention Vol.14 No.3

Aim: To study any correlation of LKB1 expression with prognosis in hepatocellular carcinoma (HCC) cases. Methods: A total of 70 HCC patients and 20 primary intrahepatic stone patients in the first affiliated hospital of Wenzhou Medical College were enrolled in this study. LKB1 expression was detected by immunohistochemistry. Patients were followed-up and prognostic factors were evaluated. Result: LKB1 expression was decreased in the HCC samples. Loss of LKB1 expression in HCC was significantly related to histologic grade (P=0.010), vascular invasion (P=0.025) and TMN stage (P=0.011). Patients showing negative LKB1 expression had a significantly shorter disease-free and overall survival than those with positive expression (P = 0.001, P=0.000, respectively). Multivariate Cox regression analysis indicated that LKB1 expression level was an independent factor of survival (P = 0.033). Conclusion: HCC patients with decreased expression LKB1 have a poor prognosis. The loss of LKB1 expression is correlated with a lower survival rate.

The Rapid Growth of the Indian Right and Its Realistic Impact1

Huang Dekai(Dekai Huang),Chen Qi(Qi Chen) 아시아사회과학학회 2022 Jornal of Asia Social Science Vol.9 No.1

Since coming into power in 2014, the Bharatiya Janata Party (BJP) has adopted a series of policies and measures to promote Hindu nationalism as the mainstream ideology of Indian politics, and has formed a comprehensive and open cooperative relationship with Hindu right-wing organizations.On the basis of combing the development of Indian right-wing organizations and summarizing the theoretical propositions of Hindu nationalism, this paper explores the influence of Hindu nationalism on India's domestic and foreign affairs.

Sensitization of Cervical Carcinoma Cells to Paclitaxel by an IPP5 Active Mutant

Zeng, Qi-Yan,Huang, Yu,Zeng, Lin-Jie,Huang, Min,Huang, Yong-Qi,Zhu, Qi-Fang Asian Pacific Journal of Cancer Prevention 2014 Asian Pacific journal of cancer prevention Vol.15 No.19

Paclitaxel is one of the best anticancer agents that has been isolated from plants, but its major disadvantage is its dose-limiting toxicity. In this study, we obtained evidence that the active mutant IPP5 ($8-60hIPP5^m$), the latest member of the inhibitory molecules for protein phosphatase 1, sensitizes human cervix carcinoma cells HeLa more efficiently to the therapeutic effects of paclitaxel. The combination of $8-60hIPP5^m$ with paclitaxel augmented anticancer effects as compared to paclitaxel alone as evidenced by reduced DNA synthesis and increased cytotoxicity in HeLa cells. Furthermore, our results revealed that $8-60hIPP5^m$ enhances paclitaxel-induced G2/M arrest and apoptosis, and augments paclitaxel-induced activation of caspases and release of cytochrome C. Evaluation of signaling pathways indicated that this synergism was in part related to downregulation of NF-${\kappa}B$ activation and serine/threonine kinase Akt pathways. We noted that $8-60hIPP5^m$ downregulated the paclitaxel-induced NF-${\kappa}B$ activation, $I{\kappa}B{\alpha}$ degradation, PI3-K activity and phosphorylation of the serine/threonine kinase Akt, a survival signal which in many instances is regulated by NF-${\kappa}B$. Together, our observations indicate that paclitaxel in combination with $8-60hIPP5^m$ may provide a therapeutic advantage for the treatment of human cervical carcinoma.

8-60hIPP5^m-Induced G2/M Cell Cycle Arrest Involves Activation of ATM/p53/p21^cip1/waf1 Pathways and Delayed Cyclin B1 Nuclear Translocation

Zeng, Qi-Yan,Zeng, Lin-Jie,Huang, Yu,Huang, Yong-Qi,Zhu, Qi-Fang,Liao, Zhi-Hong Asian Pacific Journal of Cancer Prevention 2014 Asian Pacific journal of cancer prevention Vol.15 No.9

Protein phosphatase 1 (PP1) is a major serine/threonine phosphatase that controls gene expression and cell cycle progression. The active mutant IPP5 ($8-60hIPP5^m$), the latest member of the inhibitory molecules for PP1, has been shown to inhibit the growth of human cervix carcinoma cells (HeLa). In order to elucidate the underlying mechanisms, the present study assessed overexpression of $8-60hIPP5^m$ in HeLa cells. Flow cytometric and biochemical analyses showed that overexpression of $8-60hIPP5^m$ induced G2/M-phase arrest, which was accompanied by the upregulation of cyclin B1 and phosphorylation of G2/M-phase proteins ATM, p53, $p21^{cip1/waf1}$ and Cdc2, suggesting that $8-60hIPP5^m$ induces G2/M arrest through activation of the ATM/p53/$p21^{cip1/waf1}$/Cdc2/cyclin B1 pathways. We further showed that overexpression of $8-60hIPP5^m$ led to delayed nuclear translocation of cyclin B1. $8-60hIPP5^m$ also could translocate to the nucleus in G2/M phase and interact with $pp1{\alpha}$ and Cdc2 as demonstrated by co-precipitation assay. Taken together, our data demonstrate a novel role for $8-60hIPP5^m$ in regulation of cell cycle in HeLa cells, possibly contributing to the development of new therapeutic strategies for cervix carcinoma.

Identification of differentially expressed miRNAs after Lactobacillus reuteri treatment in the ileum mucosa of piglets

Qi Wang,Qian Sun,Jing Wang,Xiaoyu Qiu,Renli Qi,Jinxiu Huang 한국유전학회 2020 Genes & Genomics Vol.42 No.11

Background Lactobacillus reuteri I5007 possesses many excellent probiotic characteristics in piglets. miRNA plays importantrole in host-microbiota interactions, but the mechanism by which L. reuteri I5007 regulates intestinal function throughits influence on miRNA expression is unknown. Objective This study analyzed the miRNA expression patterns in the ileum mucosa tissue of piglets by L. reuteri I5007treatment, aim to clarify its molecular mechanism for regulating intestinal function through miRNA. Methods Neonatal piglets were orally administered L. reuteri I5007 or a placebo daily starting on day 1, and differentialexpression of ileal miRNAs was analyzed at 10 and 20 days of age by small RNA sequencing. Results 361 known porcine miRNAs were identified, and ten miRNAs were highly expressed in the ileum mucosa in bothtreatments. Nineteen differentially expressed (DE) miRNAs were identified in response to L. reuteri treatment, and four DEmiRNAs (ssc-miR-196a, -196b-5p, -1285 and -10386) were differentially expressed at both time points. The KEGG pathwayanalyses showed the targets of 19 DE miRNAs were involved in 63 significantly enriched pathways, including the PI3KAktand MAPK pathways, which were confirmed to play important roles in probiotic-host communication. L. reuteri I5007exerted anti-inflammatory effects by influencing the levels of inflammatory cytokines. Suppressor of cytokine signalling 4gene was the target gene of ssc-miR-196a/-196b-5p, overexpression of ssc-miR-196a/-196b-5p downregulated the mRNAexpression of IL-1β and TNFα in IPEC-J2 cells. Conclusion Our study provides new insight into the role of miRNAs in the intestinal function of piglets after L. reuteriI5007 treatment.

Behavior Prediction Based on Vision Judgment of Ball Carrier in Basketball Matches

Huang Qi,Zou Xiaowei,Li Hui,Ma Tiemin,Lin Dong 보안공학연구지원센터 2015 International Journal of Multimedia and Ubiquitous Vol.10 No.10

The introduction of digital video technology into basketball trainings can greatly increase the efficiency of training; this thesis proposes a behavior prediction method based on the vision judgment of ball carrier to the computer technology used for the behavior prediction of ball carrier. The method analyzes the characteristics in basketball matches like complexity background, fast speed, and low resolution and so on, which integrate the multiple effective head vision characteristics, form the Riemannian manifold, and then map them into tangent space. Well-trained classifier is used to recognize the head pose of the ball carrier to ensure the visual range. This thesis makes prediction experiments for shooting, passing and dribbling of the ball carrier, and the simulation experiments shows that the proposed method is correct and efficient.

Utilization of electron beam to modulate electron injection over Schottky barrier

Qi Zhang,Junjie Qi,Yunhua Huang,Huifeng Li,Xin Li,Ruoshui Wang,Yue Zhang 한국물리학회 2011 Current Applied Physics Vol.11 No.3

Modulation of electron injection over Schottky barrier was realized by employing electron beam irradiation on the metal/ZnO-nanowire contact. The structure revealed a good response to the illumination at a scanning frequency of 0.1 Hz, which can be enhanced by increasing bias and decreased with the increase of electron beam energy. These phenomena can be attributed to the Schottky barrier formed at the metal/ZnO-nanowire interface which limits the electron transport across the contact. The electron beam irradiation gives rise to high efficiency of electron injection over the barrier, while the interplay between charge discreteness, coherent scattering, and Coulomb interaction may reduce the conductivity.

Commutation Torque Ripple Suppression in Three Phase Brushless DC Motor Using Open-end Winding

Qi Huang,Ling Luo,Yuan Zhang,Jichao Cao 제어·로봇·시스템학회 2021 International Journal of Control, Automation, and Vol.19 No.8

Three phase brushless DC motor has the characteristics of high power density, simple structure and excellent speed regulation performance, which is widely used in the field of electric drive. Open-end winding brushless DC motor has no neutral point, and each phase winding is electrically isolated, which can realize the independent control of each phase winding. Compared with Y-connected brushless DC motor, the open-end winding brushless DC motor has a wider range of speed regulation under the same voltage, and has certain fault-tolerant performance, which is suitable for the application of low voltage and large current. Compared with the multiphase fault-tolerant motor, open-end winding brushless DC motor has simpler structure and higher winding utilization rate. However, the open-end winding brushless DC motor still has torque ripple, among which the cogging torque is related to the motor body structure, while commutation torque ripple is affected by the mutual inductance ofthe winding and the current freewheeling loop. The commutation process of the open-winding brushless DC motor is analyzed, and it is concluded that the current change rates of the turn-off phase and turn-on phase are equal under the ideal back electromotive force(back-EMF), which can ensure that the non-commutation phase current and the electromagnetic torque kept constant. The overlapping commutation with phase current closed-loop method is proposed to suppress the commutation torque ripple of open-end winding BLDC motor, and the applied voltage on turn-off phase and delayed time of turn-off phase in overlapping commutation are derived, the sliding mode observer is used to calculate the electromagnetic torque. MATLAB simulation and experimental results verify the effectiveness of this method.

Design and research of deep slot universal motor for electric power tools

Qi, Huang,Ling, Luo,Jichao, Cao,Wei, Xi The Korean Institute of Power Electronics 2020 JOURNAL OF POWER ELECTRONICS Vol.20 No.6

In this paper, a deep slot universal motor is designed for electric power tools. The deep slot structure can reduce the cost of universal motor materials and improve production efficiency. A mathematical model of a universal motor is established and a simulation model is established by MagNet finite element software. Then, the static magnetic field distribution and load characteristics of the deep slot universal motor are analyzed. Different methods are adopted to improve the commutation spark of a deep slot universal motor. Finally, three 550-W and 9500-rpm prototype motors are tested. Simulation and experimental results indicate that an uneven air gap, brush offset by a certain angle, as well as different turns of the lead coil and the lag coil can effectively improve the commutation spark of a deep slot universal motor.

Assessment of the relative tectonic activity of the Longxian–Baoji Fault Zone in the northeastern Tibetan Plateau based on geomorphic indices

Qi Huang,Xiaohu Zhou,Jiyuan You,Shuaishuai Xu,Lushan Liu,Yang Wang 한국지질과학협의회 2023 Geosciences Journal Vol.27 No.6

Since the Late Cenozoic, tectonic deformation has been intense in the Longxian–Baoji Fault Zone (LBFZ), which is at the intersection of the southwest margin of the Ordos Block, the northeast margin of the Tibet Plateau, and the Qinling Orogenic Belt. To evaluate the relative tectonic activity within the LBFZ and discuss the influence of the northeastward expansion of the Tibetan Plateau on the geomorphological evolution of the LBFZ, we extracted data of the Qianhe, Hengshuihe, and Jinlinghe River Basins from the ASTER GDEM and analyzed the geomorphic indices, including the hypsometric integral (HI), standardized stream length-gradient index (SL/K) and Hack profile, elongation ratio (Re), the drainage basin asymmetry factor (AF) and valley floor width-to-height ratio (VF). Through geomorphic indices and field investigations, we found that the LBFZ has experienced relatively high tectonic activity. Combined with the index of relative active tectonics (IAT), we compared the tectonic activity strengths of the four major faults in the study area. Among them, the tectonic activities of the Longxian-Qishan Fault (LQF) and the Taoyuan-Guichuansi Fault (TGF) are the highest. The morphology and AF values of the drainage basin of the southwest side of the study area indicate the influence of northwest-southeast compressive stress. The northeastward expansion of the Tibetan Plateau affected the LBFZ region, and the stress brought about by it controlled the tectonic deformation in the region and sculpted the modern landscape. This study is of great significance for understanding the impact of the northeastward expansion of the Tibetan Plateau on the geomorphological evolution of the LBFZ.