Three cytotoxic compounds isolated from the stems of Parthenocissus tricuspidata Planch

Cao Li Hua,Jung JiHyun,Fang Zhe,Lee WiJae,Jung EunJoo,Son JongKeun,Lee ChongSoon,Woo MiHee 대한약학회 2004 大韓藥學會 總會 및 學術大會 Vol.2004 No.1

Allogeneic Fibrin Clot for Odontogenic/Cementogenic Differentiation of Human Dental Mesenchymal Stem Cells

Cao Hua-Lian,Chung Jong Hoon,Choung Pill-Hoon 한국조직공학과 재생의학회 2020 조직공학과 재생의학 Vol.17 No.4

BACKGROUND: Fetal bovine serum is widely used as a growth supplement for cell culture medium; however, animal-borne pathogens increase the risk of transmitting infectious agents. Platelet-rich fibrin is recently considered as a successful alternative but leukocytes present limits to its allogeneic feasibility. The aim of this study was to explore the effects of allogeneic fibrin clot (AFC) without leukocytes on inducing odontogenic/cementogenic differentiation of human dental pulp stem cells (hDPSCs) and human periodontal ligament stem cells (hPDLSCs) in vitro and in vivo. METHODS: AFC was prepared by high-speed centrifugation and leukocytes were almost removed, and AFC serum was obtained through three freeze–thaw cycles. hDPSCs and hPDLSCs were treated with AFC serum to investigate the odontogenic or cementogenic associated markers by real-time polymerase chain reaction. hDPSCs were treated with AFC serum and placed inside of dentin canal, hPDLSCs were treated with AFC serum to wrap outside of dentin, the mixture was then transplanted into the subcutaneous of nude mice for 12 weeks. RESULTS: AFC serum exhibited enough growth factors and cytokines to induce odontogenic/cementogenic differentiation of hDPSCs and hPDLSCs in vitro. Furthermore, AFC seurum could induce hDPSCs to differentiate into odontoblasts-like cells and pulp-like tissues, and hPDLSCs to regenerate cementum-like tissues. CONCLUSION: AFC could be an alternative safe source with growth factors for the expansion of human dental mesenchymal stem cells (hDMSCs).

Quantification of three-dimensional facial asymmetry for diagnosis and postoperative evaluation of orthognathic surgery

Cao, Hua-Lian,Kang, Moon-Ho,Lee, Jin-Yong,Park, Won-Jong,Choung, Han-Wool,Choung, Pill-Hoon Korean Association of Maxillofacial Plastic and Re 2020 Maxillofacial Plastic Reconstructive Surgery Vol.42 No.-

Background: To evaluate the facial asymmetry, three-dimensional computed tomography (3D-CT) has been used widely. This study proposed a method to quantify facial asymmetry based on 3D-CT. Methods: The normal standard group consisted of twenty-five male subjects who had a balanced face and normal occlusion. Five anatomical landmarks were selected as reference points and ten anatomical landmarks were selected as measurement points to evaluate facial asymmetry. The formula of facial asymmetry index was designed by using the distances between the landmarks. The index value on a specific landmark indicated zero when the landmarks were located on the three-dimensional symmetric position. As the asymmetry of landmarks increased, the value of facial asymmetry index increased. For ten anatomical landmarks, the mean value of facial asymmetry index on each landmark was obtained in the normal standard group. Facial asymmetry index was applied to the patients who had undergone orthognathic surgery. Preoperative facial asymmetry and postoperative improvement were evaluated. Results: The reference facial asymmetry index on each landmark in the normal standard group was from 1.77 to 3.38. A polygonal chart was drawn to visualize the degree of asymmetry. In three patients who had undergone orthognathic surgery, it was checked that the method of facial asymmetry index showed the preoperative facial asymmetry and the postoperative improvement well. Conclusions: The current new facial asymmetry index could efficiently quantify the degree of facial asymmetry from 3D-CT. This method could be used as an evaluation standard for facial asymmetry analysis.

Li Qingzhao and Religion

CAO Xin-hua 한국문학과종교학회 2016 한국문학과종교학회 국제학술대회 Vol.2016 No.07

李清照与佛教的关系问题,迄今为止少人关注。 其自号“易安居士”并非仅仅出于自命清高,从现有史 料来看,家世变故、无子、亲朋影响等是促成其接近佛教的主要原因。从其诗词文的用典、境界、意象 等还可发现其对佛教的态度。借助佛教这一视角,李清照诗词中的意象选择、修辞手段及其风格等问题 都可得到新的诠释。 李清照正是以其独特的人生体验走近佛学,借以解脱人生苦痛,并有机地融佛学思 想于其作品之中。 其词作的悲凉、哀艳之美正源于此。 “李清照对宗教是冷漠的”之说法值得商榷。 女居士 文人与佛教之关系是个值得深入探究的课题。

Prognostic Value of Baseline and Interim Total Metabolic Tumor Volume and Total Lesion Glycolysis Measured on 18F-FDG PET-CT in Patients with Follicular Lymphoma

Jin-Hua Liang,Yun-Ping Zhang,Jun Xia,Chong-Yang Ding,Wei Wu,Li Wang,Lei Cao,Hua-Yuan Zhu,Lei Fan,Tian-Nv Li,Jian-Yong Li,Wei Xu 대한암학회 2019 Cancer Research and Treatment Vol.51 No.4

Purpose The purpose of this study was to investigate the prognostic significance of total metabolic tumor volume (TMTV) and total lesion glycolysis (TLG) in patients with follicular lymphoma (FL) at baseline and mid-treatment with 18F-fluorodeoxyglucose positron emission tomography computed tomography (PET-CT) scans. Materials and Methods The study analyzed data from 48 patients with FL who were treated in Jiangsu Province Hospital and reviewed their baseline PET-CT scans. TMTV and TLG were computed by using the absolute value of 2.0, 2.5, and 3.0 thresholding method, respectively. Results Median age was 53 years, 75.0% of patients had stage III to IV disease, 43.8% had a Follicular Lymphoma International Prognostic Index 1 (FLIPI1) score of 3 to 5 and 20.8% had a FLIPI2 score of 3 to 5. Receiver operating characteristic (ROC) curve analysis showed the optimal cut-off values for TMTV3.0 and TLG3.0 were 476.4 (sensitivity, 85.7%; specificity, 78.0%; area under the curve [AUC], 0.760; p=0.003) and 2,676.9 (sensitivity, 71.4%; specificity, 78.0%; AUC, 0.760; p=0.003). On multivariable analysis, TMTV3.0 and TLG3.0 were independent predictors of both progression-free survival (PFS) (hazard ratio [HR], 5.406; 95% confidence interval [CI], 1.326 to 22.040; p=0.019 and HR, 6.502; 95% CI, 1.079 to 39.182; p=0.042) and overall survival (OS) (HR, 4.111; 95% CI, 1.125 to 15.027; p=0.033 and HR, 5.885; 95% CI, 1.014 to 34.148; p=0.049). ROC curve analysis showed the optimal cut-off values for TMTV3.0 and TLG3.0 were 66.3% (sensitivity, 85.7%; specificity, 63.4%; AUC, 0.774; p < 0.001) and 64.5% (sensitivity, 85.7%; specificity, 65.9%; AUC, 0.777; p < 0.001). Conclusion Baseline TMTV and TLG are strong predictors of PFS and OS in FL. Furthermore, interim TMTV (TMTV > 66.3%) and TLG (TLG > 64.5%) reduction are valuable tools for early treatment response assessment in FL patients.

An Adaptive Energy Aware Algorithm for Reliable Transmission Sensor Networks

Shi-Hua Cao,Qi-Hui Wang,Li-Dong Wang 보안공학연구지원센터 2016 International Journal of Grid and Distributed Comp Vol.9 No.8

Route maintenance and repair process plays an important role in mobile self-organizing network. On the basis of considering route stability and energy aware routing protocol, a new scheme is proposed in this paper. Firstly, signal strength, rest energy and port queue length are periodically and alternately detected; secondly, cross-layer method is adopted for route maintenance, and the alternate route is actively established for data transmission according to the routing repair mechanism “Make-before-Break”; finally, relevant experiment is carried out in NS2 to simulate and compare the proposed protocol and other similar routing protocols such as RSEA and LAER. The experiment result shows that the new scheme has good performance in the aspects of data packet delivery ratio, overhead control and end-to-end delay.

Doping effect on the adsorption of Na atom onto graphenes

Li-Hua Yao,Wen-Qiang Cao,Mao-Sheng Cao 한국물리학회 2016 Current Applied Physics Vol.16 No.5

We investigate the adsorption of Na atoms on pristine graphene and four types of defect graphenes using the first-principles calculations. The adsorption energies, electronic structures, Na storage capacities and the average potentials of Na atoms on different graphenes are calculated. The adsorption energies of Na atoms on B-doped, vacancy and B-doped vacancy graphenes are 1.93 eV, 2.46 eV, and 2.08 eV, respectively, which are much lower than that of Na on the pristine graphene (0.71 eV) and Na on Ndoped graphene (0.27 eV). The orbital hybridizations in the B-doped, vacancy and B-doped vacancy graphenes can be observed from the partial density of states, while there is no obvious orbital hybridization in the N-doped graphene as well as the pristine graphene. The B-doped vacancy graphene has the best Na storage capacity, while B-doped and vacancy graphenes also possess better Na storage capacities than those of the pristine graphene and N-doped graphene. This work demonstrates that the graphene introducing both B dopant and vacancy is expected to be a potential material for storing Na.

Requirement for ERK Activity in Sodium Selenite-induced Apoptosis of Acute Promyelocytic Leukemia-derived NB4 Cells

( Bing She Han ),( Wei Wei ),( Fang Yuan Hua ),( Ting Ming Cao ),( Hua Dong ),( Tao Yang ),( Yang Yang ),( Hua Zhen Pan ),( Cai Min Xu ) 생화학분자생물학회 2007 BMB Reports Vol.40 No.2

Targeting the IL-1β/IL-1Ra pathways for the aggregation of human islet amyloid polypeptide in an ex vivo organ culture system of the intervertebral disc

Cao Yang,Xinghuo Wu,Zhiwei Liao,Kun Wang,Wenbin Hua,Xianzhe Liu,Yu Song,Yukun Zhang,Shuhua Yang 생화학분자생물학회 2019 Experimental and molecular medicine Vol.51 No.-

Intervertebral disc degeneration (IDD) is characterized by excessive apoptosis of nucleus pulposus (NP) cells and hyperactive extracellular matrix (ECM) catabolism. Our previous studies revealed the relationship between human islet amyloid polypeptide (hIAPP) and NP cell apoptosis. However, the role of hIAPP aggregates in IDD has not yet been investigated. This study aimed to determine whether the accumulation of hIAPP aggregates promotes IDD progression. The aggregation of hIAPP increased in human NP tissues during IDD. The deposition of hIAPP aggravated the compression-induced IDD that promoted NP cell apoptosis and ECM degradation via IL-1β/IL-1Ra signaling in an ex vivo rat disc model. Moreover, neutralizing IL-1β augmented the protective effects of hIAPP overexpression by decreasing hIAPP aggregation in human NP cells. These results suggest that the aggregation of hIAPP promotes NP cell apoptosis and ECM degradation ex vivo and in vitro by disrupting the balance of IL-1β/IL-1Ra signaling.

Effects of contraction-type impeller on non-overloaded performance for low-specific-speed sewage pump

Hua Zhang,Bin Chen,Wei-dong Shi,Zhong-yong Pan,Wei-dong Cao 대한기계학회 2014 JOURNAL OF MECHANICAL SCIENCE AND TECHNOLOGY Vol.28 No.3

With the expansion of the engineering application of solid-liquid two-phase flow, the overload characteristics of low-specific-speedsewage pumps become an important obstacle to the development of the products. In this paper, the traditional diffusion-type impeller isutilized to carry out hydraulic design of a low-specific-speed sewage pump. And on this basis, the thickness of the blades is increased forgetting an impeller with contractive-type flow channel. It was found out that through external characteristics, the efficiency of contraction-type impeller is slightly lower than that of diffusion-type impeller, but it shows obvious non-overload performance. PIV experimentis arranged to find the inherent. The results show that although internal flow field distribution of contraction-type impeller is better thanthat of diffusion-type impeller, the severe uneven distribution of absolute velocity of the impeller outlet along the circumference is one ofmain reasons of inefficiency. In addition, another five impellers with different contraction ratio are designed to research relationship betweencontraction ratio and character of non-overload. The characteristics are predicted by CFD, and after comparing the results, it isfound out that, the smaller the contraction ratio is, the closer the condition of power extreme is to the design condition, which has importantimplications for the engineer.