TTC및 Penzym방법에 의한 우유중 Antibiotic Residues의 검출에 관한 연구

양철영,이수한 한국축산식품학회 1994 한국축산식품학회지 Vol.14 No.1

국내에서 널리 사용되고 있는 세균 발육 억제 물질 검출 시험의 일종인 dye 지시약으로 2, 3, 5- triphenyltetrazolium chloride을 이용한 방법과 속성 검출 시험 방법인 penzym kit를 이용한 방법으로 원유, 시유의 항생물질 검출성, 시험방법간의 검출능 비교·고찰한 내용은 다음과 같다. TIC시험에 의한 원유중의 항생물질 검출성은 3개 시험구 60시료에서 평균 25.00%의 양성율을 나타내고, 평균 시험 소요시간은 198.25 분이었고, penzym시험에 의한 양성율은 43.33%이고 소요시간은 31.35분을 필요로 하였다. 시유 및 가공유중의 검출성은 15시료의 TIC시험에 의한 경우 13.33%의 양성율과 penzym시험의 경우 46.66%의 양성율을 나타내고 있다. TIC 시험방법에서 penicillin G 는 0.03 IU/㎖, cloxa-cillin은 1.0 × 10^(-l0)g/㎖, oxacillin, ampicillin 및 sulfa-methazine은 1.0 × 10^(-15)g/㎖까지 검출이 가능하였으며 penzym 시험방법에서는 penicillin G 는 0.001 IU /㎖oxacillin, cloxacillin, sulfamethazine은 1.0 × 10^(-15)g/㎖, ampicillin은 1.0 × l0^(-11)g/㎖까지 검출성을 보였다. Penzym 시험에 의한 검출방법은 특이성, 신속성, 간편성 및 재현성이 있음을 실험결과에서 나타내었다. Antibiotic residues in raw milk and city milk were detected by 2,3,5-triphenyltetrawlium-2H-choride test method(TTC) and penzym test method, and the penzym test and TTC test for antibiotic detection were compared. The results obtained are summarized as follows: Raw milk, inhibitor positive were 25.00% and 43.33% of 60 sample by TIC test and penzym test, and experimental require time was 198.25 minutes and 31.35 minutes, respectively. Market milk, inhibitor positive were 13.33% and 46. 66% of 15 sample by TIC test and penzym test. TTC test was detected 0.03 IU/㎖ of penicillin G, and oxacillin, ampicillin and sulfamethazine was detected 1.0 × 10^(-15)g/㎖, and more than 1.0 × l0^(-l0)g/㎖ of cloxacillin, penzym test was detected 0.001 IU/㎖ of penicillin G, and oxacillin, cloxacillin and sulfamethazine was detected 1.0 × 10^(-15)g/㎖ and more than 1.0 × l0^(11)g/㎖ of ampicillin. This study showed various merit such as specificity, fastness, simple handling and reapperance by penzym test.

工場現地化戰略에 관한 硏究

梁漢洙,朴徹敏,朴光日 慶南大學校 産業經營硏究所 1996 産業經營 Vol.19 No.-

競爭戰略과 生産戰略의 適合性에 관한 硏究 : 電子産業을 中心으로

梁漢洙,朴光日 경남대학교 산업경영연구소 2000 産業經營 Vol.27 No.-

우리나라 農村 保健支所에서 高血壓患者의 追求管理 方案 및 그 效果에 關한 硏究

韓聖鉉,金琅昊,李成秀 순천향대학교 1987 논문집 Vol.10 No.2

As a follow-up of the case-control study of some risk factors and status of hypertension control in rural community undertaken in May 1985, a brief assessment survey was carried out in May 1987 in order to evaluate the effectiveness of a special hypertension clinic set up at a health sub-center in Eumseong community for one year period, beginning from April 1986. The objectives of this paper were : (1) To describe a hypertension control model designed for primary care at health sub-center level (2) To present the results of follow-게 care of 39% of hypertension for one year in terms of the change in health indicator(BP level) and the factors related to compliance & non-compliance with prescribed regimen. For this study, data was obtained from 24 hypertension patients diagnosed in the previous study, dividing them into two groups : 42% as the compliance group(C-group) and 58% as the noncomplance group(NC-group). In comparing the two groups, the following results were obtained : 1. There were some difference between the two groups. In their characteristics : For example, (a) in sex composition, C-group was consisted of more female than NC-group(60% vs 50%) (b) C-group had shorter history of hypertension than NC group : the proportion of patients under 4 years group(60% vs 36%) 2. During one year period, 24 sessions of special hypertension clinics were operated at HSC. But the mean number of visits of C-group was 21.9, while NC group had 5.3 visits. 3. Knowledge & attitude toward the needs of hypertension treatment were not different between both groups. Also there were less appreciation on the prescribed duration of care to comply in both groups. 4. C-group had expressed their reliance on the treatment at the special clinic services at HSC, but NC-group *) This investiation was surpported by 1986 research grant of Soonchunhyang University. O.K. also partially sup ported by the GTZ grant. had less relied on the special clinic services at HSC and more prefered to the clinic services of the hospital. But there patients attended hospital were not referred back to HSC. 5. Main reason for compliance of C-group was their concern to prevent from stroke attack and other CV diseases. The reasons for NC-group were mainly due to (1) Geographical in accessibility, (2) Economic problems to pay, (3) Less satisfaction on progress in treatment. 6. As a results of this program in average level of BP, it was found that an average BP of C-group were decreased from 187.6㎜Hg in systolic BP and from 112.2㎜Hg to 103.4㎜Hg in diastolic BP. But, the same of NC-group increased from 169.1㎜Hg to 176.0㎜Hg in systolic & form 100.0㎜Hg to 102.3㎜Hg in diastolic BP. While it is too early to claim the success of special clinic program for hypertension control at primary care(HSC) level, this assessment survey led us a tentative conclusion that public doctor assigned at health sub-center can reasonably manage the hypetension control program if there are some support to overcome clients problems in economic & geographic accessibility and if patients adequately comply with the each special clinic sessions at HSC. In order to further strengthen the hypertension control program at primary care level, the following recommendation can be made ; (1) A set of portable EKG instrument should be equipped at health sub-center when public physicians wish to initate the community-based hypetension control program. (2) Taking into account the size of population & geographical area of each health sub-center(or township), and their work load, the HSC can inititate the H.C. program, starting from the accesible villages near HSC where & when their villages wish to participate in the program. (3) To formulate an effective community-based hypertension control program, there is a need of systematic referral system between HSC(primary) & Hospital(secondary), linking the available health resources in the community(e.g. VHW-CHP-HSC-GP-Hospital) as now being developed by the Eumseong CH project.

古時調의 感歎詞 小考 : 終章 初句를 中心으로

漢陽洙 東國大學校 국어국문학부 1965 國語國文學 論文集 Vol.6 No.-

금융기관 CRM 운영 사례와 구축 전략

양한수 慶南大學校 産業經營硏究所 2002 産業經營 Vol.31 No.-

연구개발과 마케팅의 연계전략

양한수,이상복 경남대학교 경남지역문제연구원 2003 경남지역연구 Vol.9 No.-

APS에 의한 생산계획수립에 있어서 TOC활용에 관한 소고

양한수,박대봉 慶南大學校 産業經營硏究所 2001 産業經營 Vol.28 No.-

製造企業의 JIT시스템 適用과 生産戰略

梁漢洙,朴鍾珖 慶南大學校 産業經營硏究所 1992 産業經營 Vol.15 No.-

Lipopolysaccharide, Dexamethasone, 및 N-Nitro-L-Arginine Methyl Ester가 흰쥐 간 조직의 프리라디칼 발생과 제거에 미치는 영향

한기정,김석배,김양균,윤혜영,백광진,이희성,권년수 중앙대학교 의과대학 의과학연구소 2003 中央醫大誌 Vol.28 No.1

Lipopolysaccharide (LPS) stimulates various immune and inflammatory reactions to induces the generation of reactive oxygen intermediates (ROI) and reactive nitrogen intermediates (RNI). ROI and RNI are free radicals with very high reactivity, oxidize cellular constituents including proteins and lipid membranes, and thus play important roles in the pathogenesis of tissue damages in LPS-induced septic shock. The present study examined effects of dexamethasone (DexM), and N-nitro-L-arginine methyl ester (L-NAME) on the generation of ROI and RNI, and on the activities of enzymes removing ROI in the liver of rat model of septic shock. DexM has widely been used as an immunosuppressant. L-NAME has known to block synthesis of nitric oxide (NO), the main RNI, by inhibiting NO synthase (NOS). LPS (10 mg/kg body weight) markedly enhanced NO generation in serum and liver. The increased NO generation was completely blocked by pretreatment with DexM (10 mg/kg) and effectively inhibited by L-NAME (10 mg/kg). Induction of NOS protein expression by LPS was demonstrated by a Western analysis. The NOS expression was completely blocked by DexM pre-treatment, and markedly inhibited by L-NAME. However, the generation of superoxide radical, an important ROI, was not enhance in rat liver by LPS, rather it was decreased a little. Superoxide radical production was increased by L-NAME treatment or combined treatment of DexM and LPS. The level of lipid peroxidation, an index for free radical-induced cell damage, was increased by either LPS or DexM. The enhancement of lipid peroxidation by DexM was abolished by adding LPS. DexM markedly enhanced the activities of ROI removing enzymes, superoxide dismutase, glutathione peroxidase and catalase. However, rats were treated with LPS in addition to DexM, the elevated activities of superoxide dismutase, glutathione peroxidase and catalase were abolished. These results provide information on the generation and removal of ROI and RNI by LPS, DexM and L-NAME in rat liver, and on development of drugs to inhibit free radical-induced liver damage in sepsis.