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Ghim, Sa-Youl Korean Society of Life Science 2001 Journal of Life Science Vol.11 No.2
The pyrR gene of the pyrimidine biosynthesis (pyr) operon of the thermophile Bacillus caldolyticus, encoding a uracil phosphoribosyltransferase (UPRTase), turned to rely as a pyr operon regulator. It has been proposed that PyrR mediates transcriptional termination-antitermination at three intercistronic regions of the par operon (S.-Y Ghim and J. Neuhard, J. Bacteriol.,176, 3698-3707, 1994). In this research, a plasmid carrying the pyrR region of B. caldolyticus could restore a pyrimidine regulation in a pyrR mutant of B. subtilis. Expression of pyrR was found to increase 6-7 fold during pyrimidine starvation. Additionally, a highly conserved nucleotide sequence which may constitute the binding site for a PyrR protein (PyrR-binding loop) in transcript was staggested. Alternative antiterminator and terminator structures involving three conserved motifs in front of the pyrR, pyrP and pyrB genes, respectively, are proposed to account for the observed regulation pattern.
RAPID PURIFICATION AND MOLECULAR CHARACTERIZATION OF HUMAN NEUTROPHIL ELASTASE
Ghim, Sa-Youl,Bae, Sung Jun,Kang, Kooil 고신대학교 의학부 1989 高神大學校 醫學部 論文集 Vol.5 No.1
Elastase에 의해 야기되는 질병의 예방 및 치료 응용을 위하여는, 먼저 이 효소가 고순도로 분리되고 그 근본적인 성질의 해명이 이루어져야 한다. 고순도의 elastase를 얻기위해, 사람의 전혈로부터 Hypaque-Ficoll step gradient에 의해 순수한 neutrophil을 얻은 다음 이것을 extract로 만들어 액체크로마토그래피를 실시하였다. Ultrogel AcA54 gel filtration을 거친 후 CM-sephadex 이온교환 크로마토그래피를 적용하였는데, 이때 유출 완충액 속에 0.1% Brij 35를 첨가하여 elastase의 순도를 더욱 높일 수 있었다. 이 정제된 elastase는 SDS-PAGE를 통해 MW 28,000, 29,000, 30,000의 서로 다른 3개 의 밴드로 확인되었으며, 그간 수많은 연구자들간에 elastase의 분자량에 대해 심한 견해 차이를 보여온 것은 그 분자적 성질이나 분자량이 유사한 cathepsin G가 혼재하거나 elastase분자내 혹은 분자간 S-S결합의 차이에 의함을 알 수 있었다.
INHIBITION OF HUMAN NEUTROPHIL ELASTASE BY DIFFERENT CLASSES OF ANTIBIOTICS
Ghim, Sa-Youl,Kang, Kooil 고신대학교 의학부 1988 高神大學校 醫學部 論文集 Vol.4 No.1
사람의 혈액에 있는 호중구 엘라스테이즈에 관한 연구의 진척은, 약물로 사용가능한 생체내 혹은 인공합성의 저해제 개발을 동반해 오고 있다. 정제된 호중구 엘라스테이즈에 12종의 항생제를 처리하였더니 20mM 미만의 lC_(50)을 보인 것은 oxytetracycline, cefamandole, gentamicin, amikacin, cloxacillin, methicillin, chloramphenicol 등이었고, 10mM 농도에서 50% 이상의 저해율을 나타낸 것은 methicillin, cefamandole, oxytetracycline 등이었다. 그 결과는 세포벽 합성이나 단백질 합성을 저해하는 일반적 항생제의 약리학적 기전과는 다른 차원에서 항생제가 숙주의 조직을 보호할 수 있는 가능성을 보여준 모델이 존재한다는 것을 in vitro 실험에서 확인할 수 있었다.
ADVANCED TREATMENT OF LEACHATE BY MEMBRANE PROCESSES
GHIM, Shijun,OZAKI, Hiroaki,TERASHIMA, Yutaka 嶺南大學校 環境問題硏究所 1996 環境硏究 Vol.16 No.1
AbstractIn this research, we present the treatment results ofleachates from landfill sites of municipal solid wastes or artificial incineration ash leachate by the process of ASMF (activated sludge process with microfiltiation membrane) combined with LPRO (low pressure reverse osmosis membrane). The LPRO showed a high removal of recaldttant substances and inorganic salts. However, this process is not appropriate fer the direct leachate treatment. Thus, the ASMF using ceramic membrane of the pore size of 0.1 fim was applied as a pretreatment process.As the result of experiments, it was found that these processes could effectively remove both recalcitrant substances and inorganic salts of leachates. For example, the treatment efficiencies of artificial. incineration ash leachate by ASMF combined with LPRO were 99 % ofCODcr, 55 %ofT-N, 70 % of calcium, and 53 % of sodium. Therefere, it was confirmed that the process of ASMF combined with LPRO can become an alternative to the physico-chemical process in conventional leachate treatment plant. In this research, we present the treatment results of leachates from landfill sites of municipal solid wastes or artificial incineration ash leachate by the process of ASMF (activated sludge process with microfiltration membrane) combined with LPRO (low pressure reverse osmosis membrane). The LPRO showed a high removal of recalcitrant substances and inorganic salts. However, this process is not appropriate for the direct leachate treatment. Thus, the ASMF using ceramic membrane of the pore size of 0.1㎛ was applied as a pretreatment process. As the result of experiments, it was found that these processes could effectively remove both recalcitrant substances and inorganic salts of leachates. For example, the treatment efficiencies of artificial incineration ash leachate by ASMF combined with LPRO were 99% of COD_cr, 55% of T-N, 70% of calcium, and 53% of sodium. Therefore, it was confirmed that the process of ASMF combined with LPRO can become an alternative to the physico-chemical process in conventional leachate treatment plant.
Synthetic Biology for Biofuels: Building Designer Microbes from the Scratch
Ghim, Cheol-Min,Kim, Tae-Sung,Mitchell, Robert J.,Lee, Sung-Kuk 한국생물공학회 2010 Biotechnology and Bioprocess Engineering Vol.15 No.1
The ultimate goal in the production of biofuels is to produce fuels identical or similar to petroleum-derived transportation fuels more efficiently and in commercial quantities. Synthetic biologists have been engineering microbes to synthesize biofuels, such as butanol and fatty acid- or isoprenoid-based fuels, which are nearly identical to gasoline and diesel. One of the most urgent demands along this direction is to attain a solid framework for characterizing and standardizing the biological parts and devices. It seems quite promising because biotechnologies specially based on miniaturizations have been making a big contribution to this work. Therefore, in this review, recent advances and difficulties in the biofuel field are discussed, along with the advances of synthetic biology, which will make it possible to create designer microorganisms that produce economically viable next generation biofuels, aside from bioethanol, from corn or sugar cane, and biodiesel from plant or animal oils.
Spectrally resolved white-light interferometry for 3D inspection of a thin-film layer structure
Ghim, Young-Sik,Kim, Seung-Woo The Optical Society 2009 Applied Optics Vol.48 No.4
<P>We describe an improved scheme of spectrally resolved white-light interferometry, which provides 3D visual inspection of a thin-film layer structure with nanometer level resolutions. Compared to the authors' previous method [Appl. Phys. Lett.91, 091903 (2007)APPLAB0003-695110.1063/1.2776015], 3D tomographic information of thin films can be obtained by decoupling the film thickness and top surface profile, which is embodied by inducing spectral carrier frequency to the reference arm and applying a low-pass filter to the interferogram instead of two troublesome measurement steps of activating and deactivating a mechanical shutter. We test and verify our proposed method by measuring a patterned thin-film layer structure as well as standard specimens of thin films with various thicknesses.</P>