μ-Oxo 및 μ-L-Proline(O,O') 및 tpen (=N,N,N',N'-tetrakis(2-pyridylmethyl)-1,2-diaminoethane)이 배위된 이핵 Fe(Ⅲ)착물의 합성과 그 특성에 관한 연구

윤두천, 윤정민, 오창언 영남대학교 기초과학연구소 2000 基礎科學硏究 Vol.20 No.-

Recently, iron-containing proteins have attracted much attention owing to their ability of dioxygen transport and storage, so many model complexes have been synthesized. Dinuclear Fe(III) complex with μ-oxo and μ-O,O'-bridging L-proline has been prepared; [Fe2(μ-O)(μ-L-proline)2(tpen)](PF6)2 (tpen=N,N,N',N'-tetrakis(2-pyridylmethyl)-1,2-diaminoethane). The structure of the dinuclear complex was identified by various spectroscopic method. The electrochemical characterization of the dinuclear complex was investigated by cyclic voltammetry. The L-proline-bridged complex exhibit one electron reduction waves. The complex confirmed that the total number of electrons involved in the reduction is one; Fe2(III, III) → Fe2(III, II). When acid(p-toluenesulfonic acid) add to [Fe2(μ-O)(μ-L-proline)2(tpen)]2+, the splitting wave was observed in acetonitrile.

?? 착물의 구조적 특성

김복조,윤두천,박기훈,주언정,오창언 嶺南大學校 基礎科學 硏究所 1998 基礎科學硏究 Vol.18 No.-

Five-coordinated complex of Cu(Ⅱ) complex, [CuCl(1,10-phenanthroline)₂]·Cl·6H₂O·CH₃OH, was synthesized by adding an aqueous solution of Cu(Ⅱ) to a methanol solution of 1,10-phenanthroline. Its crystal structure was detemined by single crystal X-ray crystallography. Crystal data are are as fllow: [CuCl(phen)₂]·Cl·6H₂O·CH₃OH, monoclinic, space group C2/c (#15), a = 23.309(5), b = 30.270(6), c = 7.491(2) A, B = 97.83(2)˚, V = 5236 A³, Z = 8,2745 data with I >3σ(I) (R = 00.64, Rw = 0.087). In the crystal structure of ??, the Cu(Ⅱ)is five-coordianted with four nitrogen and one chlorine atom. The geometry around Cu(Ⅱ) is somewhat distorted trigonal bipyramid and Cu(Ⅱ) is locted nearly (0.0280 A˚) on the least-squares plane of the two nitrogen atoms and one chlorine atom.

용리제에 의한 금속착물의 크로마토그래피적인 분리

吳昌彦,尹斗千 嶺南大學校附設 基礎科學硏究所 1986 基礎科學硏究 Vol.6 No.-

The elution behaviors of cationic complexes were determined by measuring retention volumes on SP-Sephadex C-25 cation exchange resin. When cationic complexes were eluted in the low concentration of eluents, their elution order was accordance with the order of their increasing charges. At this time, the elution depends on the strength of adsorptivities between cationic complex and the resin. However the elution order of cationic complexes was reserved when they were eluted in the high concentration of eluents. On this occasion, cationic complexes have been eluted predominantly by ion-association between cationic complex and eluent. Hydrophilic complexes were associated more strongly with chloride ion than bromide ion. But hydrophobic complexes were opposed to above. In the comparision of sulfate ion and halide ions(Cl- and Br-), hydrophilic complexes were associated more easily with sulfate ion than halide ions(Cl- and Br-), and hydrophobic complexes were opposed to above.

Crystal Structures and Characterization of Copper(II) Complexes of N,N,N\'N\'-Tetrakis(2-pyridylmethyl)-1,2-ethanediamine

Doo Cheon Yoon,Uk Lee,Chang Eon Oh* 대한화학회 2004 Bulletin of the Korean Chemical Society Vol.25 No.6

Crystal Structures and Characterization of Copper(II) Complexes of N,N,N'N'-Tetrakis(2-pyridylmethyl)-1,2-ethanediamine

Yoon, Doo-Cheon,Lee, Uk,Oh, Chang-Eon Korean Chemical Society 2004 Bulletin of the Korean Chemical Society Vol.25 No.6

The structure of [Cu(tpen)]$(ClO_4)_2$ (tpen = N,N,N',N'-tetrakis(2-pyridylmethyl)-1,2-ethanediamine) has been identified by X-ray crystallography. The copper(II) ion is surrounded by two amine N atoms and three pyridine N atoms of the ligand, making a distorted trigonal-bipyramid. Among the six potential N donor atoms (two amine N and four pyridine N atoms), only one pyridine N atom remains uncoordinated. We examined structural changes on addition of $Cl^-$ to $[Cu(tpen)]^{2+}$(1). The addition of $Cl^-$ in methanol resulted in the formation of a novel dinuclear copper(II) complex $[Cu_2Cl_2(tpen)](ClO_4)_2{\cdot}H_2O$. The structure of the dinuclear complex was verified by X-ray crystallography. Each copper(II) ion in the dinuclear complex showed a distorted square planar geometry with two pyridine N atoms, one amine N atom and one $Cl^-$ ion.

Synthesis and X-ray Structural Study of a 6-Coordinate Copper(II) Complex with N,N,N',N'-Tetrakis(2-pyridylmethyl)-1,2-ethanediamine

Yoon, Doo-Cheon,Lee, Uk,Lee, Dong-Jin,Oh, Chang-Eon Korean Chemical Society 2005 Bulletin of the Korean Chemical Society Vol.26 No.7

New selectable host–marker systems for multiple genetic manipulations based on TRP1, MET2 and ADE2 in the methylotrophic yeast Hansenula polymorpha

Cheon, Seon Ah,Choo, Jinho,Ubiyvovk, Vera M.,Park, Jeong-Nam,Kim, Moo Woong,Oh, Doo-Byoung,Kwon, Ohsuk,Sibirny, Andriy A.,Kim, Jeong-Yoon,Kang, Hyun Ah John Wiley Sons, Ltd. 2009 Yeast Vol.26 No.9

<P>Interest has been increasing in the thermotolerant methylotrophic yeast Hansenula polymorpha as a useful system for fundamental research and applied purposes. Only a few genetic marker genes and auxotrophic hosts are yet available for this yeast. Here we isolated and developed H. polymorpha TRP1, MET2 and ADE2 genes as selectable markers for multiple genetic manipulations. The H. polymorpha TRP1 (HpTRP1), MET2 (HpMET2) and ADE2 (HpADE2) genes were sequentially disrupted, using an HpURA3 pop-out cassette in H. polymorpha to generate a series of new multiple auxotrophic strains, including up to a quintuple auxotrophic strain. Unexpectedly, the HpTRP1 deletion mutants required additional tryptophan supplementation for their full growth, even on complex media such as YPD. Despite the clearly increased resistance to 5-fluoroanthranilic acid of the HpTRP1 deletion mutants, the HpTRP1 blaster cassette does not appear to be usable as a counter-selection marker in H. polymorpha. Expression vectors carrying HpADE2, HpTRP1 or HpMET2 with their own promoters and terminators as selectable markers were constructed and used to co-transform the quintuple auxotrophic strain for the targeted expression of a heterologous gene, Aspergillus saitoi MsdS, at the ER, the Golgi and the cell surface, respectively. The nucleotide sequences presented here were submitted to GenBank under Accession Nos AY795576 (HpTRP1), FJ226453 (HpMET2) and FJ493241 (HpADE2), respectively. Copyright © 2009 John Wiley & Sons, Ltd.</P>

Hg²+에 의한 cis-〔Co(en)₂(L)CI²+](L=아민류) 착물의 아쿠아화 및 킬레이트화 반응

尹斗千,金福祚,都命基,吳昌彦 嶺南大學校 基礎科學硏究所 1992 基礎科學硏究 Vol.12 No.-

수용액 및 유기용매-물 혼합용매속에서 L이 NH₃,NH₂CH₃,??, glyOCH₃, ??, NH₂CH₂CONH₂,이 배위된 ?? (en=1,2-diaminoethane)에 ??을 작용시켰을때 속도론적인 자료, 원평광이색성 스펙트라, 이온교환 크로마토그래피에 의한 생성물 분석 및 용매의 성질인 Y(ionizing power)를 이용한 m(Grunwald-Winstein의 기울기) 값의 결과들로 부터 반응은 ??-메카니즘으로 제안되었다. 반응 결과 L이 NH₃과 NH₂CH₃ 이 배위된 ??은 Cl이 해리되어 H₂O분자가 치환되었으며, L이 ??,glyOCH₃, ??, NH₂CH₂CONH₂과 NH₂CH₂CN이 배위된 ??은 Co(Ⅲ)에 대한 각 리간드 L의 킬레이트화가 일어났다. 에탄올-물 혼합용매 속에서 ??을 제외한 ??은 에탄올의 양이 증가될수록 속도상수(k) 의 값이 증가되었고, 또한 30% 유기용매-물 혼합용매 속에서의 속도상수의 값이 30% 2-프로판올-물>30% 에탄올-물>물의 순서를 나타내었다. 그러나 ??은 이와 반대의 결과를 나타내었다. 각 착물들의 속도상수의 차이에 있어서 ?? 및 ??은 리간드장 파라메타(△)와 관계가 있었지만, ??(L=??,glyOCH₃, ??, NH₂CH₂CONH₂, NH₂CH₂CN)은 이와 관계가 없었다. ??에 대하여 ??을 첨가시켜 ??이 촉매로서 작용하는 반응에서 ??에 의하여 그 반응 메카니즘이 변화되는 것이 아니라 단지 속도만 변화되었다. It has been suggested that ??-promoted reaction of a series of?? (en=1.2-diaminoethane with L=NH₃,NH₂CH₃,??, glyOCH₃??, NH₂CH₂CONH₂, and NH₂CH₂CN proceeds by dissociative interchange(??) mechanism from kinetic data, circular dichroism spectra, analyses of products, and the values of m(Grunwald-Winstein plot) using Y (solvent ionizing power) in aqueous solution and in mixed aqueous-organic solvent. It has been found that chloride replacement by water (aquation) for the series with L=NH?? and NH??CH?? and chelation of ligand L to Co(Ⅲ) for the series with L=??, glyOCH₃,??, NH₂CH₂CONH₂, and NH₂CH₂CN occurs, respectively. The rate constants on ??-induced reaction of the series except ?? were increased with increasing the contents of ethanol in mixed water-ethanol solvents. In mixed water-30% organic solvents, the rate constants of the series except ?? have also been measured in the order 30% 2-propanol-water>30% ethanol-water>water. However, the rate constants of ?? were reversed. The rate constants of the series with L=NH?? and NH??CH?? were related to ligand field parameter (△), but those of the series with L=??, glyOCH₃, ??, NH₂CH₂CONH₂, NH₂CH₂CN were not.That reaction between the series and ?? in aqueous media containing NO₃ has been investigated. The results for the reaction do not alter the mechanism, but the rate only was altered.

Zipeprol(레스피렌®)을 탐닉하던 노인의 급성 중독 사망례

이두환 ( Doo Hwan Lee ),최상천 ( Sang Cheon Choi ),안정환 ( Jung Hwan Ahn ),조영신 ( Young Shin Cho ),김기운 ( Gi Woon Kim ),민영기 ( Young Gi Min ),정윤석 ( Yoon Seok Jung ) 대한임상독성학회 2009 대한임상독성학회지 Vol.7 No.2

Zipeprol dihydrochloride is a non-opioid mucolytic, antitussive agent and it is frequently prescribed for respiratory symptoms such as cough and sputum. The main pharmacologic mechanisms of zipeprol are inhibition of superior laryngeal nerve stimulation and direct antagonism for stimulation of the bronchial receptors, which might have an effect for the drug’s mucolytic action. Many cases of drug abuse with zipeprol have occurred world-wide due to the hallucinogenic effect of the drug. In Korea, zipeprol was reported to be the most commonly abused drug among young people for the 1990s. Zipeprol associated death was first reported since 1991 and 69 cases of death related to zipeprol abuse were further reported during 8 years (between 1991 and 1998). In addition to the hallucinogenic effect, dyspnea, extrapyramidal symptoms, seizure, cerebral edema have been reported as the signs and symptoms of toxic zipeprol overdose. However, zipeprol abuse is not common for old age people and non drug abusers. We report here on a fatal case of acute zipeprol poisoning in an eighty five year old drug addicted woman.

비처리 연골과 냉동건조 연골의 변형에 대한 비교 연구

최해천,윤근철,강정훈,오두영 大韓成形外科學會 1998 Archives of Plastic Surgery Vol.25 No.6

Human untreated costal cartilage was compared with lyophilized human costal cartilage which was treated with defatting solution for 48 hours and freeze drying for 72 hours (-70℃, 10??bar) on the tendency of distortion. All cartilages, which were taken from six cadavers, were carved 5×5×30mm in size on principle of the balanced cross section. Their distortions were evaluated by two methods at intervals of one week, one month, three months, six months after experiment. At first, the degree of distortion was grossly graded with four steps: grade O; no distortion / grade Ⅰ; minimal distortion / grade Ⅱ; moderate distortion / grade Ⅲ; severe distortion. Second method is measurement and quantification of distortion in the horizontal and vertical plane of cartilage. Untreated cartilage is shown to be an unsatisfactory material, with only three(12%) of the 25 cartilages being cosmetically acceptable(grade 0 and 1) after 6 months. In lyophilized cartilage, 18(94%) of the 19 cartilages were cosmetically acceptable(grade 0 and 1) at 6 months. This figure is highly significant(p<0.01). In another method, distortion in the horizontal(h) and vertical (v) planes of cartilage were measured, and mean values of ???? were calculated. In untreated group, the mean values of ???? were 0.82 at 1 week, 0.91 at 1 month, 1.13 at 3 months, and 1.31 at 6 months. In lyophilized group, the mean values were 0.27 at 1 week, 0.29 at 1 month, 0.40 at 3 months and 0.47 at 6 months. All values were statistically significant(p<0.01).