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Raphanus sativus Var. Chungpihongsim의 hairy root 배양에 의한 anthocyanin 생성
황성진,백윤웅,고경민,안준철,황백 全南大學校 農漁村開發硏究所 1991 農業科學技術硏究 Vol.26 No.-
청피홍심무우를 재료로 A. rhizogenes A₄균주를 접종하여 hairy root를 유도하였으며 유도된 hairy root는 paper electrophoresis를 통하여 mannopine과 agropine이 검출되어 형질전환 되었음을 확인하였다. Hairy root는 MS의 기본염을 1/2로 희석시킨 1/2 MS, sucrose 3%, pH 5.2로 조성된 배지에서 최적인 성장을 보였다. 유도된 hairy root는 각 strain사이에 색소능에 다소간의 차이를 보였으며 2, 4-D kinetin의 적절한 조합의 처리구에서는 탈분화와 더불어 색소가 형성되었고, 그 중 2, 4-D와 kinetin을 각각 1 ppm, 5 ppm 처리하였을 때 최대 색소능을 보였다. hairy root의 탈분화시 형성되는 색소는 원 식물 뿌리의 주 색소인 pelargonidin 계열의 색소로 추정되며 총 색소량은 49mg/100g(F.W.)로 잠정 계산되었다. Hairy root induced following inoculation of the root disc and plantlet fragment of R. sativus Var Chungpihongsim with A. rhizogenes A₄. The hairy root contained agropine and mannopine. Medium contained 1/2 MS salt, sucrose 3%, pH 5.2 was optimized to growth of hairy root. Depending on the hairy root line, the level of pigment in hairy root varied somewhat . When 2, 4-D and kinetin were added in hairy root, the synthesis of pigment induced with deorganization of hairy root. Especially, addition of 2, 4-D 1 ppm and kinetin 5 ppm showed the maximum synthesis of pigment. Compared with anthocyanin extract of mature root three pigments were assumed to acylated pelargonin. The content of anthocyanin in these sample was 49mg/100(F.W.).
소형 기기용 효율적인 바이트 코드 검증기술에 관한 연구
황철준,조증보,정민수 慶南大學校 附設 工業技術硏究所 2003 硏究論文集 Vol.21 No.2
USIM(Universal Subscriber Identity Module) 기술은 데이터의 관리와 보호를 하나의 소형 칩 내에 집약시킨 기술로 최근에 이 기술에 대한 관심도가 증가하고 있다. 자바카드 가상기계는 자바 언어로 작성된 프로그램이 USIM 칩 내부에서 실행 가능하도록 해준다. 하지만 자바카드 가상 기계는 자원 제약적인 디바이스의 특성으로 인하여 검증기술을 제공하지 않는다. 본 논문에서는 정적 체크 기반의 효율적인 바이트코드 검증기를 제안한다. 연구 결과 본 논문에서 제안하는 검증기는 일반적인 것에 비해 90% 이상의 사이즈가 감소되었다. As Universal Subscriber Identity Module (USIM) technology can handle, manager and protect data within a small sized chip, peopled are more interested in USIM technology lately. Java Card Virtual Machine (JCVM) enables programs written by Java programming language to be run in smart cards with USIM chips. However JCVM does not have a verifier as limits of hardware. In this paper, we propose a ByteCode verifier with novel algorithm based on Static Check policy. We experiment that the size of our verifier is reduced by 90% over generals.
Design and Implementation of Web Server Communication Protocol for Java USIM Card
Cheul-Jun Hwang,Tae-Heon Noh,Min-soo Jung 한국멀티미디어학회 2008 한국멀티미디어학회 국제학술대회 Vol.2008 No.-
USIM is embedded on mobile internet equipments or user certification systems. Its main purpose is user certification and storing sensitiveness dates. Since USIM computing power (cups, memory space) has been enhanced recently and USIM has an open platform structures, we can develop various application services for USIM card. For this reason, we suggest a new web based USIM Interface service - WebUSIM. This system is working as a small Web Server thus TCP/IP packet processing and http message services are enabled. Since WebUSIM supports TCP/IP function, it can provide seamless types of web application services by USIM card. For WebUSIM, we suggest a new IP over IS07816 communication protocol. IP over ISO7816 can provide more powerful Web services on USIM card.
황철준(Cheul-Jun Hwang),박진호(Jin-Ho Park),정민수(Min-soo Jung) 한국멀티미디어학회 2009 한국멀티미디어학회 학술발표논문집 Vol.2009 No.1
USIM은 사용자 개인의 민감한 데이터와 응용 서비스를 저장 가능하며 다양한 보안 알고리즘의 적용으로 뛰어난 보안적 특성 때문에 그 활용 분야가 점차 증대되고 있다. 이러한 USIM을 보다 편리하게 접근하기위한 인터페이스 시스템에 대해 논의한다.
홈 네트워크 환경에서의 안전한 인증 메커니즘에 대한 연구
황철준(Cheul-Jun Hwang),노태헌(Tae-Heon Noh),정민수(Min-soo Jung) 한국멀티미디어학회 2008 한국멀티미디어학회 학술발표논문집 Vol.2008 No.1
정보 통신망과 디지털 가전 제품의 등장과 초고속망의 사용자가 기하급수적으로 증가하고 있는 현 추세에서 홈 네트워크는 다양한 인프라 시스템을 활용하여 발전하고 있다. 홈 네트워크 환경은 기존의 네트워크 환경과는 차별적으로 다양한 잠재적인 보안 취약성이 존재한다. 홈 네트워크 연결의 시작점이라고 불 수 있는 홈 게이트웨이 환경에서 사용자 인증 메커니즘은 다양한 측면에서 고려되어야 한다. 첫째 서비스가 동적으로 실행되는 컴포넌트 환경인 이라는 것, 둘째 한번의 인증으로 여러 서비스의 사용이 가능한 편의성의 제공 마지막으로 홈 게이트웨이 시스템의 자원적 제약성을 고려한 최적화된 환경에 대한 고려가 필요하겠다. 이러한 환경을 고려하여 본 논문에서는 홈 게이트웨이 환경 중에서 개방형 모델을 추구하는 OSGi 프레임워크 상에서의 보안 인증 메커니즘에 대해 연구하였다.
미치광이풀 ( Scopolia parviflora ) 의 모상근 배양에 의한 Tropane Alkaloid 생산
안준철(Jun Cheul Ahn),정병균(Byung Gyun Jung),백윤웅(Yun Woong Paek),김영준(Young Jun Kim),고경민(Kyeong Min Ko),황성진(Sung Jin Hwang),황백(Baik Hwang) 한국식물학회 1993 Journal of Plant Biology Vol.36 No.3
Transformed hairy roots of Scopolia parviflora, prducing tropane alkaloids and native to Korea, were obtained following infection of rhizome segments with Agrobacterium rhizogenes A_4. Each root tip induced from inoculum sites excised and cultured in MS agar or liquid medium. About seventy of hairy root clones were established. Among them, several fast growing hairy root clones were examined for alkaloid content. Two dimensional TLC analysis showed that the tropane alkaloid pattern of hairy root was more complicated than that in the rhizome of mother plant. On the other hand, some hairy root clones did not produce scopolamine and hyoscyamine. In HPLC analysis, some hairy root clones yield higher levels of scopolamine and hyoscyamine than those of mother plant rhizome which used for infection. Scopolamine and hyoscyamine were identified by comparison of their retention times and of their mass spectra data with those of authentic compounds.
Hwang, Byungtae,Lee, Sang-Hyun,Kim, Jang-Seong,Moon, Ji Hyun,Jeung, In Cheul,Lee, Na Geum,Park, Jongjin,Hong, Hyo Jeong,Cho, Young-Lai,Jung, Haiyoung,Park, Young-Jun,Lee, Seon-Jin,Lee, Hee Gu,Kim, Won Elsevier 2015 Biomaterials Vol.51 No.-
<P><B>Abstract</B></P> <P>Angiopoietin-1 (Ang1) and its endothelium-specific receptor, tyrosine kinase with Ig and epidermal growth factor homology domain 2 (Tie2), play critical roles in vascular development. Although the Ang1/Tie2 system has been considered a promising target for therapeutic neovascularization, several imitations of large-scale production have hampered the development of recombinant Ang1 for therapeutics. In this study, we produced a fully human agonistic antibody against Tie2, designated 1–4h, and tested the applicability of 1–4h as an alternative to native Ang1 in therapeutic angiogenesis. 1–4h significantly enhanced the phosphorylation of Tie2 in a dose- and time-dependent manner in human Tie2-expressing HEK293 cells and human umbilical vein endothelial cells. Moreover, 1–4h induced the activation of Tie2-mediated intracellular signaling such as AKT, eNOS, MAPK, and Focal Adhesion Kinase p125<SUP>FAK</SUP>. In addition, 1–4h increased the chemotactic motility and capillary-like tube formation of endothelial cells <I>in vitro</I> and enhanced the survival of serum-deprived endothelial cells. Taken together, our data clearly suggest that a human Tie2 agonistic antibody is a potentially useful therapeutic approach for the treatment of several ischemic diseases including delayed-wound healing and ischemic heart and limb diseases.</P>
Hwang, Ok-Jin,Kim, Yu-Jeong,Sung, Nak-Sul,Ahn, Jun-Cheul,Kim, Sik-Eung,Hwang, Baik The Korean Society of Medicinal Crop Science 2002 한국약용작물학회지 Vol.10 No.4
The effects of basal media, carbon, nitrogen, phosphate and some major macro elements on growth and shikonin production in Lithospermum erythrorhizon hairy root culture were studied. Among examined media, growth of hairy root cultured in B5 liquid medium was rapid, whereas shikonin production was high in MS liquid medium. Under B5 basal medium, sucrose concentration for optimal growth and shikonin production was 9% and 4% respectively. The growth and shikonin production on pH changes in B5 medium resulted little effect in pH 5.8 to pH 8.8 ranges, whereas growth was decreased dramatically in both above 8.8 and under 5.8. Nitrogen source and concentration effected on the growth and shikonin production. The highest growth rate was in B5 medium (50 mM $KNO_3$ and 1 mM $NaH_2PO_4)$, whereas the highest shikonin production was in the condition supplemented with 5 mM $KNO_3$ and 10 mM $NaH_2PO_4$.
시호 ( Bupleurum falcatum L. ) 의 모상근 배양을 통한 Saponin 생산 - 1. 캘러스 , 부정근 , 모상근과 재배근의 Saponin 양상과 함량 비교
안준철(Jun Cheul Ahn),백윤웅(Yun Woong Paek),성충기(Chung Ki Sung),강근형(Geun Hyung Kang),황백(Baik Hwang) 한국식물학회 1993 Journal of Plant Biology Vol.36 No.1
In order to survey possibility to produce saikosaponin from in vitro hairy root culture, culture of callus, adventitous root, and hairy root of Bupleurum falcatum L. were estabilished, and quantitative and qualitative aspects in saikosaponin extracted from thses were compared with those of cultivated root. Callus grew well in MS medium containing 0.9 ㎛ 2,4-D. In contrast, both of adventitous root and hairy root grew well in hormone-free MS medium. However, hairy root showed more rapid growth with extensive lateral root branches, characteristics of lower content of water and softer than in adventitous root. Among the selected lines of adventitous root and hairy root were observed difference in the growth rate. Mannopine, one of opine synthesized in the transformed tissue with Agrobacterium rhizogenes A_4 were detected in the extract of hairy root lines. Pattern and content of crude saponin from adventitous and hairy root showed no difference, but somewhat difference from those of cultivated root. However, in callus, distinct production-aspect of saponin was not observed.
청피홍심무우 ( Raphanus sativus cv. Chungpihongsim ) 의 모상근 배양에 의한 안토시아닌 생성
안준철(Jun Cheul Ahn),백윤웅(Yun Woung Paek),강영희(Young Hee Kang),황백(Baik Hwang) 한국식물학회 1992 Journal of Plant Biology Vol.35 No.1
The hairy root culture of Raphanus sativus cv. Chungpihongsim was established by transformation with Agrobacterium rhizogenes A_4. The transformed roots grew well in adjusted Murashige and Skoog medium to 1/2 basal salts, pH5.2,3% sucrose. Agropine and mannopine, poine synthesized in the transformed tissue were detected in the extract of hairy roots. When 2,4-D and kinetin were added in culture medium of hairy roots, the synthesis of anthocyanin was induced with disorganization of hairy root. Especially, addition of 0.45 μM 2,4-D and 2.3 μM kinetin showed the maximum synthesis of anthocyanin. Pattern of anthocyanin synthesized in transformed roots was somewhat different from that of ordinary roots. However, aglycone part of all anthocyanin was identified as pelargonidin. The content of total anthocyanin in this sample was tentatively calculated 0.49 mg/g fresh weight.