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이두형,최병열,장원철,서길수,이태진 영남대학교 공업기술연구소 1999 工業技術硏究所論文集 Vol.27 No.1
Mesoporous MCM-41 exibits a hexagonal arrangement of uniform mesopores whose dimensions may be engineered in the range of ∼20Å to greater than 100Å. The MCM-41 typically have high surface areas above 1000m2/g, and then capacities of MCM-41 to accommodate the larger hydrocarbon is still higher than the general molecular sieve. At the present, many surfactants as organic templates have been proposed for various formations of these MCM-41. In this study, we synthesized mesoporous MCM-41 which has a spherically hexagonal structure. Also, the pore size of MCM-41 could be changed by the control of alkyl chain length of surfactants.
차병열(Byung Yul Cha),홍병규(Byung Que Hong),조현수(Hyun Su Jo),손호선(Haw Son Sohn),박영철(Yeong Chyll Park),양원석(Won Seok Yang),최옥인(Ok In Choi) 한국수산과학회 1997 한국수산과학회지 Vol.30 No.1
Stomach contents of 518 specimens of the yellow goosefish, Lophius litulon caught by the off-shore stownet in the southern waters of Korea were examined by means of the calculation of the frequency of occurrence, number and weight percentages, and index of relative importance (IRI) in each prey organisms. Prey organisms of the yellow goosefish consist of fishes, crustaceans, cephalopods. Fishes were dominant prey group, and occupied 98.8% in IRI of prey organisms. Four species of fish, Pseudosciaena manchurica, Collichthys niveatus, Engraulis japonica, Trichiurus lepturus were dominant as to the IRI and occupied 87.2% of the total IRI. P. manchurica was a dominant prey organism in predator`s stomach through the year, and the other dominant prey organisms occurred intermittently with seasonal progress. P. manchurica was a dominant prey organism to all fish size, too. C. niveatus and E. japonica decreased in importance in the diet as the fish size increased, concomitant with an increase in importance of T. lepturus. Mean species number and mean individual number of prey organisms increased with the fish size.
Choi, Jae Young,Roh, Jong Yul,Li, Ming Shun,Shim, Hee Jin,Kang, Joong Nam,Woo, Soo Dong,Jin, Byung Rae,Je, Yeon Ho Korean Society of Sericultural Science 2004 International Journal of Industrial Entomology Vol.9 No.2
Recently, we have developed an easy, simple and convenient circular DNA cloning system named plasmid capture system (PCS). To investigate usefulness of PCS in cloning of plasmids from Bacillus thuringiensis strains, PCS donors, pPCS-S and pPCS-L were applied to clone plasmids of B. thuringiensis subsp. israelensis by in vitro transposition using 4{TnsABC^*}$ transposase. In result, 3 small plasmids were cloned, and these were consistent with pTX14-1, pTX14-2 and pTX14-3 reported previously from B. thuringiensis subsp. israelensis. Therefore, the PCS can be successfully applied to clone small plasmids from B. thuringiensis strains.
( Yul Hee Cho ),( Seok Hui Kang ),( Yaeni Kim ),( Myung Hyun Lee ),( Gun Hee An ),( Byung Ha Chung ),( Bum Soon Choi ),( Chul Woo Yang ),( Yong Soo Kim ),( Yeong Jin Choi ),( Cheol Whee Park ) 대한신장학회 2013 Kidney Research and Clinical Practice Vol.32 No.3
Background: Nephrotic syndrome (NS) and proteinuria are uncommon, often unrecognized manifestations of graft-versus-host disease (GVHD) after hematopoietic stem cell transplantation (HSCT). Only a few isolated case reports and case series involving smaller number of patients who developed NS after HSCT have been published. Methods: We reviewed the renal histopathological examination findings and clinical records of 15 patients who developed proteinuria after HSCT at Seoul and Yeouido St. Mary′s Hospital (Seoul, Korea). We also measured the anti-PLA2R antibodies (M-type phospholipase A2 receptor) in the serum samples from the seven patients at the time of renal biopsy. Results: All patients had GVHD. The most common indication for biopsy was proteinuria (41 g/day), with nine patients having nephrotic range proteinuria. The most common histopathological finding was membranous nephropathy (MN; n ¼ 12). Other findings were membranoproliferative glomerulonephritis, C1q nephropathy, and diabetic nephropathy. Eleven patients were treated with immunosuppressive agents, and three patients were treated only with angiotensin II receptor blocker. The overall response rate, including complete remission (urinary protein level o0.3 g/day) and partial remission (urinary protein level ¼ 0.31?3.4 g/day), was 73%. The mean follow-up period was 26 months, and none of the patients developed end-stage renal disease. All of the seven patients with MN had negative findings for anti-PLA2R antibodies, measured using an enzyme-linked immunosorbent assay kit. Conclusion: In this study the findings of 15 renal biopsies were analyzed and to our knowledge this is the largest clinicopathological study of GVHD-related biopsy-proven nephropathy. Approximately 80% of the patients were MN and 73% responded either partially or completely to immunosuppressive treatment. Currently, there is an increase in the incidence of GVHD-mediated renal disease, and therefore, renal biopsy is essential for diagnosing the nephropathy and preventing the progression of renal disease.
Improved baculovirus vectors expressing barnase using promoters from Cotesia plutellae bracovirus
Choi, Jae Young,Kim, Yang-Su,Wang, Yong,Kang, Joong Nam,Roh, Jong Yul,Shim, Hee Jin,Woo, Soo-Dong,Jin, Byung Rae,Je, Yeon Ho Springer-Verlag 2009 Molecules and cells Vol.28 No.1
<P>The goal of this study was to create a novel baculovirus expression system that does not require recombinant virus purification steps. Transfection of insect cells with transfer vectors containing barnase under control of the Cotesia plutellae bracovirus (CpBV) promoters ORF3004 or ORF3005 reduced cell growth. Co-transfection with bApGOZA DNA yielded no recombinant viruses and non-recombinant backgrounds. To further investigate the detrimental effects of barnase on insect cells, two recombinant bacmids harboring the barnase gene under control of the CpBV promoters, namely bAcFast-3004ProBarnase and bAcFast-3005ProBarnase, were constructed. While no viral replication was observed when only the recombinant bacmids were transfected, recombinant viruses were generated when the bacmids were co-transfected with the transfer vector, pAcUWPolh, through substitution of the barnase gene with the native polyhedrin gene by homologous recombination. Moreover, no non-recombinant backgrounds were detected from unpurified recombinant stocks using PCR analysis. These results indicate that CpBV promoters can be used to improve baculovirus expression vectors by means of lethal gene expression under the control of these promoters.</P>
Sequence and gene organization of 24 circles from the Cotesia plutellae bracovirus genome
Choi, Jae Young,Kwon, Soo-Jin,Roh, Jong Yul,Yang, Tae Jin,Yoon, Sook Hee,Kim, Heebal,Li, Ming Shun,Park, Beom-Seok,Woo, Soo-Dong,Jin, Byung Rae,Kim, Yonggyun,Je, Yeon Ho Springer-Verlag 2009 Archives of virology Vol.154 No.8
Choi, Jae-Young,Wang, Yong,Kim, Yang-Su,Kang, Joong-Nam,Roh, Jong-Yul,Woo, Soo-Dong,Jin, Byung-Rae,Je, Yeon-Ho 한국응용곤충학회 2008 Journal of Asia-Pacific Entomology Vol. No.
To investigate the usefulness of early-expressed promoters from Cotesia plutellae bracovirus(CpBV) in improvement of insecticidal activities of baculovirus, recombinant AcNPVs, Ac3003ProAalT, Ac3004ProAalT, Ac3005ProAaIT and Ac3006ProAalT expressing scorpion neurotoxin, AaIT, under the control of ORF3003, ORF3004, ORF3005 and ORF3006 promoter, respectively were constructed. Among these recombinant viruses, Ac3006ProAaiT showed highest insecticidal activity against 3rd instar larvae of Spodoptera exigua. These results suggested that early promoters from CpBV could be successfully applied to improve pathogenicity of baculoviruses.