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홍웅길,안준혁,정경원,나재운 한국공업화학회 2018 한국공업화학회 연구논문 초록집 Vol.2018 No.0
Branched polyethylenimine (bPEI) has many amine group. it used high gene transfection agent. However, bPEI utilization as gene carrier was limited because of high cytotoxicity. To overcome these, chitosan was introduced to bPEI by carbonyldiimidazol as coupling agent. The chemical structure of chitosan-g-bPEI analyzed by 1H-NMR. The binary and ternary complexes particle size, zeta potential and mophological property was confirmed by DLS, zeta sizer and TEM. Moreover, transfection efficiency and cellular uptake showed that ternary complexes more than binary complexes. The targeting effect of ternary complexes was confirmed by competition assay in CD44-receptor overexpressed HCT116 cell line.
Application of Tropolone-encapsulated chitosan nanoparticles
홍웅길,안준혁,정경원,나재운 한국공업화학회 2019 한국공업화학회 연구논문 초록집 Vol.2019 No.0
Tropolone has been used as preserved agent in food. However, utilization of tropolone as preserved agent has been limited because of expensive cost and short half-life. To overcome these obstacle, tropolone was encapsulated to chitosan-graft stearic acid micelle (CSA), which may lead to sustained release of tropolone. The chemical structure of CSA was analyzed by <sup>1</sup>H-NMR. Moreover, tropolone loaded CSA nanoparticles (CSAT) were prepared by evaporation method. The contents, encapsulation efficiency and release behavior of tropolone from CSAT were confirmed by UV-vis spectrophotometer. Its particle size and morphological property of CSA and CSAT were confirmed by DLS and TEM. Moreover, cytotoxicity of CSA and CSAT were confirmed by MTT assay in human embryo kidney (HEK 293) cell line. These results suggest that CSA micelle is exceptional drug carrier for sustained release of tropolone. (No. NRF-2016H1D5A1910499).
홍웅길,남충현,안준혁,정경원,박준규,나재운 한국공업화학회 2017 한국공업화학회 연구논문 초록집 Vol.2017 No.1
Reactive oxygen species (ROS) have been caused to life-span due to many diseases and aging. The anti-oxidant (AO) used for the removal of ROS has a potential such as Anti-allergic, anti-oxide, anti-cancer effective and Low toxic. However, the disadvantages of AO is that it has a low bioavailability in the body. Therefore, we developed to natural anti-oxidant-encapsulated chitosan microsphere by spray dry method. Its chemical structure was analyzed by <sup>1</sup>H-NMR and FT-IR. In addition, morphological property of anti-oxidant agent-encapsulated chitosan microsphere (AECM) was analyzed by SEM and It showed to spherical shape. Beside, particle size of AECM was confirmed to 2 ~ 5 ㎛. Moreover, its cytotoxicity was accomplished by MTT assay. Additionally, encapsulation of anti-oxidant from AECM was confirmed by UV-vis spectrophotometer. These results suggest that AECM can be effectively removed ROS as an antioxidant with low toxicity and improved bioavailability. (NRF-2016H1D5A1910499).
홍웅길,정경원,김원석,최창용,나재운 한국공업화학회 2016 한국공업화학회 연구논문 초록집 Vol.2016 No.1
Branched polyethyleneimine(PEI) was well known as high gene transfection agent, However, utilization of PEI as gene carrier was limited because of high cytotoxicity. To solve this problem, PEI was introduced to chitosan(CS) back bone by using coupling reaction. Also, hyaluronic acid(HA) was introduced for targeted CD44-receptor in breast cancer cell. Its structural analysis of PEI-grafted chitosan(CSPEI) was confirmed by <sup>1</sup>H-NMR. In addition, ternary complex of pDNA/CSPEI/HA was prepared by electrostatic charge interaction. Its gel retardation assay showed that pDNA was completely condensed by CSPEI and HA. Moreover, pDNA was protected from DNase and released by heparin. Also, transfection assay showed that ternary complexes was effectively transfected more than binary complexes by targeting effect of HA. <sup>**</sup>These results suggest that ternary complexes can enhance transfection efficiency by targeted CD44-receptor in breast cancer cell. (NRF-2014R1A2A1A10053027).