불안정 애착과 관계중독의 관계: 자기통제력에 의해 조절된 내면화된 수치심의 매개효과

홍세림 ( Hong Serim ),장유진 ( Jang Yoojin ) 한국청소년학회 2023 청소년학연구 Vol.30 No.5

본 연구에서는 성인들의 불안정 애착과 관계중독의 관계에서 내면화된 수치심의 매개효과를 확인하고, 이러한 매개효과가 자기통제력 수준에 따라 변화하는지 확인하고자 하였다. 전국 20대~50대 성인 307명을 대상으로 자기 보고식 설문을 통해 자료를 수집하였고 수집된 자료는 SPSS 26.0과 PROCESS Macro를 활용하여 분석하였다. 주요한 연구결과를 요약하면 다음과 같다. 첫째, 애착불안이 높을수록 관계중독 수준이 높은 것으로 나타났으며, 애착회피는 관계중독과 유의한 관련성을 보이지 않았다. 둘째, 애착불안과 관계중독의 관계에서 내면화된 수치심이 부분 매개하는 것으로 나타났다. 셋째, 내면화된 수치심과 관계중독의 관계에서 자기통제력의 조절효과가 유의하였다. 넷째, 애착불안이 내면화된 수치심을 거쳐 관계중독에 미치는 간접효과가 자기통제력의 수준에 따라 달라지는 것으로 나타나 조절된 매개효과가 유의하였다. 연구 결과를 바탕으로 청소년 상담에서의 시사점을 제시하였으며, 연구의 제한점을 토대로 후속연구의 방향을 제언하였다. This study examined the mediating effects of internalized shame between insecure attachment and relation addiction among adults and whether these mediating effects would vary depending on levels of self-control. Data were collected using the self-report survey from 307 adults aged between their 20s to 50s. The collected data were analyzed using SPSS 26.0 and PROCESS Macro. The main results are as follows. First, an anxious attachment was positively related to relationship addiction, but the avoidant attachment was not significantly correlated with relationship addiction. Second, internalized shame partially mediated the relationship between anxious attachment and relationship addiction. Third, self-control moderated the relationship between internalized shame and relationship addiction. Fourth, the indirect effect of anxious attachment on relationship addiction via internalized shame varied depending on levels of self-control, indicating that the moderated mediation effect was significant. Based on the findings, implications for counseling adolescents were presented, as well as directions for future studies along with the limitations of this study.

소와 돼지 도축장 도체 및 환경에서 분리된 병원성대장균 분포 및 특성조사

홍세림 ( Serim Hong ),강혜정 ( Hye Jeong Kang ),문진산 ( Jin-san Moon ),윤순식 ( Soon-seek Yoon ),김하영 ( Ha-young Kim ) 한국동물위생학회 2022 韓國家畜衛生學會誌 Vol.45 No.3

We investigated the virulence genes, O-serotypes, antimicrobial resistance of pathogenic E. coli isolated from carcasses (n=455) and environmental (n=372) samples of 11 cattle and 12 pig slaughterhouses from December 2020 to December 2021. E. coli were isolated from nine carcasses (2.0%), three slaughter facilities (1.4%), two utensils (2.7%) and three abattoir workers (3.5%) from four cattle and four pig slaughterhouses. Among all isolates, 13 STEC (76.5%) were identified, followed by four EPEC (23.5%). As a result of the antibiotic susceptibility test, all isolates were resistant to at least one antimicrobial, of which 70.6% isolates showed multidrug resistance patterns. The serotypes were diverse in pigs compared to cattle, with serotypes O18, O66, O109 in cattle and O9, O76, O85, O100, O153, and O159 in pigs. In a single cattle slaughterhouse, eight STEC O66 were isolated from various types of sample (4 slaughter animal surfaces, 3 gloves, and 1 knife) with two antimicrobial resistance patterns (CHL-FIS-STR and CHL-FIS). Those two types of strain were suspected crosscontamination from utensils to slaughter animal surfaces. These results showed that pathogenic E. coli were detected in carcasses and various environmental samples in cattle and pig slaughterhouses. Nationwide monitoring and hygiene management are required to prevent cross-contamination of STEC isolate slaughterhouses.

젖소 유방염에서 분리한 Streptococcus 종의 분포 및 항생제 내성 분석

강혜정 ( Hye Jeong Kang ),홍세림 ( Serim Hong ),박다솜 ( Dasom Park ),김하영 ( Ha-young Kim ),문진산 ( Jin-san Moon ) 한국동물위생학회 2022 韓國家畜衛生學會誌 Vol.45 No.3

Streptococcus is one of the major pathogen groups inducing bovine mastitis. The aim of this study was to investigate the antimicrobial resistance patterns of Streptococcus species isolated from bovine mastitis milk samples in Korea from 2016 to 2021. In total, 181 (10.3%) Streptococcal isolates were collected from 1,761 quarter milk samples at 122 farms; S. uberis 39.2% (n=71), S. dysgalactiae 29.3% (n=53), S. equinus 9.9% (n=18), S. suis 6.1% (n=11), S. parauberis 4.4% (n=8), S. lutetiensis 3.9% (n=7), others 7.2% (n=13). However, S. agalactiae was not isolated. The isolates showed the highest resistance rate to tetracycline (55.2%) followed by erythromycin (45.3%) and pirlimycin (36.5%). In contrast, all isolates were susceptible to ceftiofur, cephalothin, penicillin/novobiocin, and only single S. equinus isolate was resistant to both ampicillin and penicillin. Of 181 isolates, 64 (35.4%) were multidrug resistance (MDR). The resistance to pirlimycin of S. uberis (73.2%) was much higher than that of other species (0∼36.4%). All S. suis isolates were resistance to tetracycline. S. dysgalactiae showed lower resistance to erythromycin, pirlimycin and tetracycline than S. uberis and S. suis. The rate of MDR was relatively higher among S. uberis (73.2%) than among S. suis (36.4%), S. dysgalactiae (15.1%), others (0%). In conclusion, antimicrobial resistance in Streptococcus spp. should be regularly examined for appropriate therapies because the resistance patterns were various among the individual species.

젖소 유방염에서 분리한 Pseudomonas spp.의 분포 및 항생제 내성 비교

강혜정 ( Hye Jeong Kang ),김하영 ( Ha-young Kim ),홍세림 ( Serim Hong ),박다솜 ( Dasom Park ),윤순식 ( Soon-seek Yoon ),문진산 ( Jin-san Moon ) 한국동물위생학회 2021 韓國家畜衛生學會誌 Vol.44 No.3

This study was aimed to investigate the prevalence and antimicrobial resistance of Pseudomonas spp. isolated from bovine mastitis milk samples. A total of 50 (4.9%) Pseudomonas spp. was isolated from 1,023 samples, those collected between 2018 and 2021, derived from 110 dairy farms. The prevalence of the identified species of Pseudomonas isolates was as follows; P. aeruginosa (70.0%), P. fluorescens (14.0%), P. putida (10.0%), P. fragi (4.0%), and P. chlororaphis (2.0%). Most of somatic cell counts in the quarter milk carrying Pseudomonas spp. were less than 3,000,000 cell/ml (90.0%). The isolates of Pseudomonas spp. showed high susceptibility to cefepime (98.0%), ciprofloxacin (98.0%), ceftazidime (96.0%), and colistin (96.0%). The rate of antibiotic resistance in the isolates was highest to ceftiofur (92.0%), followed by the resistance rate to chloramphenicol (86.0%) and trimethoprim/ sulphamethoxazole (80.0%). In addition, there is a remarkable difference in antimicrobial resistance pattern among Pseudomonas species. P. aeruginosa and P. putida showed a similar resistance pattern, whereas P. fluorescens showed exceptionally lower resistance to trimethoprim/sulphamethoxazole and chloramphenicol than that of the other species. This study showed that prevalence of Pseudomonas spp. other than P. aeruginosa were 30.0% in bovine mastitis milk, and the occurrence rate of antibiotic resistance were similar or higher level, compared with the previous reports on the mastitis- derived Pseudomonas spp. isolated in Korea.

원유 세균수 검사장비의 정도관리를 위한 표준시료의 개발 및 평가

강혜정 ( Hye Jeong Kang ),김진환 ( Jin Hwan Kim ),변영섭 ( Yeong Seob Byun ),이하나 ( Hana Lee ),이혜영 ( Hye Young Lee ),김지현 ( Jihyeon Kim ),홍세림 ( Serim Hong ),김하영 ( Ha-young Kim ),윤순식 ( Soon-seek Yoon ),문진산 ( Jin- 한국동물위생학회(구 한국가축위생학회) 2020 韓國家畜衛生學會誌 Vol.43 No.3

Standard samples were prepared for this study, and applied for BactoScan ^TM and BactoCount ^TM in order for quality control evaluation for total bacterial count in raw milk. Accordingly, the preparation of two lots of standard samples for quality control were lyophilized, which contain Lactobacillus lactis. The standard samples were prepared into three different levels of bacterial counts, those were Low 30,000∼ 40,000, Medium 70,000∼90,000, High 150,000∼220,000 CFU/mL, respectively. Then, the proficiency tests were performed in total 19 laboratories for measuring total bacterial counts. The total bacterial counts in the standard samples showed 37∼42, 82∼105, 214∼240 CFU/mL in the first lot, and it showed 30∼36, 67∼75, 131∼163 CFU/mL in the second lot in low, medium and high levels, respectively. Based on these results, the absolute values of z-scores of six standard samples in 18 laboratories were ≤2, which means the samples are satisfactory. However, z-score in one laboratory was ≤3, which means the sample is questionable. Using two standard samples, the correlation between BactoScan ^TM and BactoCount ^TM was 0.9982, which means the results of total bacterial count measurement of both equipment were equivalent. Therefore, the standard samples manufactured in this study for quality control of total bacterial count using BactoScan ^TM and BactoCount^TM in the raw milk could be applied to proficiency tests.