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대형 KTP 단결정 성장 및 광학적 불균일성에 관한 연구
한재용,이성국,마동준,김용훈,박성수,이상학,Han, J.Y.,Lee, S.K.,Ma, D.J.,Kim, Y.H.,Park, S.S.,Lee, S.H. 한국결정성장학회 1994 한국결정성장학회지 Vol.4 No.1
고온용액법(high temperature solution grwth)에 의해 $K_6P_4O_13$의 flux로 부터 KTP 단결정을 성장시켰다. Inclusion이 없는 KTP 단결정을 성장시키기 위해 성방로내 온도기울기, 결정의 회전, 종자결정의 방위, 냉각속도를 조절하였다. 성장된 KTP 단결정은 inclusion이 없었으며, 크기는 $10(a){\times}28(b){\times}33(c)mm^3$ 이었다. 또한 KTP 단결정 boule의 위치에 따른 SHG 출력 특성 측정 및 TEM 분석에 의해 종자결정 주위의 광학적 불균일성의 원인을 조사하였다. Single crystals of Potassium Titanyl Phosphate (KTP) were grown from the flux of $K_6P_4O_13(K_6)$ using a high temperatures solution growth method. To grow the large KTP crystal without inclusion, the temperature gradient in furnace, crystal rotation, orientation of seed crystal, and the cooling rate were controled. The KTP crystals are up to $10(a){\times}28(b){\times}33(c)mm^3$ in size. We investigated the optical inhomogeneity in this KTP crystal by the SHG power measurement and TEM analysis.
한재용,박성수,이성국,마동준,김용훈,이상학,Han, J.Y.,Park, S.S.,Lee, S.K.,Ma, D.J.,Kim, Y.H.,Lee, S.H. 한국결정성장학회 1994 한국결정성장학회지 Vol.4 No.4
무게 측정에 의한 자동 직경 제어 방식의 Czochralski 단결정 성장 장칭를 제작하였다. 성장로의 몸체와 hot zone을 자체 설계, 제작하였으며, computer에 의하여 전자저울과 유도가열장치를 자동 제어하므로써 가열 공정에서 부터 냉각 공정까지의 모든 과정을 자동화시킬 수 있는 program을 개발하였다. 제작된 성장장치를 사용하여 $LiNbO_3$ 단결정을 성장시켜 성장 장치의 성능을 평가 하였다. We developed a computer controlled Czochralski puller which automated entire crystal growing processes. Crystal weighing technique was employed for an automatic diameter control. The performance of the system was evaluated by the growth of $LiNbO_3$ single crystals.
닭 초기 배아의 유전자 미세주입과 유전자 발현에 관한 연구
한재용(J . Y . Han),(R . N . Shoffner),(K . S . Guise) 한국축산학회 1994 한국축산학회지 Vol.36 No.3
This study was carried out to examine the expression of marker genes and integration of plasmid DNA into the germ cells in young chicken embryos. The RSVLTR/βG2 plasmid contains the lacZ gene under the control of rous sarcoma virus (RSV) long terminal repeat(LTR) promoter. After a square window of 5㎜ per side was cut in the side of the egg with a dentistry drill, the transfection cocktail of calcium-phosphate or lipofectin with plasmid DNA was microinjected in the area of blastoderm and germinal crescent whose developmental stages were stage X and stage 6 to 8, respectively. Microinjected eggs were sealed, and the eggs were returned to the incubator with the $quot;window$quot; side up overnight. Microinjected embryos with plasmid DNA were screened with Xgal, and the marker gene was expressed in the brain, notochord and other parts of body of 1.5∼4.5 day old embryos, suggesting that developing stem cells in unincubated blastoderms or 1∼4 somite embryos can be transfected with plasmid vectors. The possibility of germ line integration with plasmid DNA by direct microinjection into early chicken embryos was determined. Transfection of stem cells for gonads in the blastoderm or germinal crescent with plasmid vectors was observed. Positive primordial germ cells in the gonad were not observed by plasmid DNA microinjection into unincubated or 24hr incubated embryos in this study. However, the expression of plasmid DNA with RSVLTR promoter in the early chicken embryonic cell shows the possibility of transgenic chicken production by direct microinjection with plasmid DNA. Also genetic manipulation of chicken production traits such as disease resistance, growth and production may he possible in the future.
RAPDs 를 이용한 육류 ( 한우육 , 사슴육 , 면양육 , 산양육 ) 의 축종 판별
민중석(J . S . Min),민병록(B . R . Min),한재용(J . Y . Han),이무하(M . Lee) 한국축산학회 1996 한국축산학회지 Vol.38 No.3
This study was performed to develop the convenient and reliable identification technique of meat species by random amplified polymorphic DNAs(RAPDs). The muscle tissue samples were taken from the KCB(Korean Cattle Beef), deer meat(DM), sheep meat(SM), and goat meat(GM). The genomic DNA of each species were extracted from these muscle tissues. The results of RAPDs had a different profile with various primers. The identification of each species was possible in the state of meat because of different band pattern of RAPDs products of each species. But the difference between SM and GM was undetectable. Species-specific band between SM and GM was shown when pooled DNA was used as template DNA, but when individual DNA was used as template DNA, it was not specific any more. This might be due to the individual difference.
MRI와 동시에 측정한 뇌전도 신호로 전류원 국지화를 할 때 경사자계 유발 잡음의 영향 분석
이항로,한재용,조민형,임창환,정현교,이수열,Lee H. R.,Han J. Y.,Cho M. H.,Im C. H.,Jung H. K.,Lee S. Y. 대한자기공명의과학회 2003 Investigative Magnetic Resonance Imaging Vol.7 No.2
목적 : MRI를 촬영하면서 동시에 측정한 뇌전도 신호에 가장 크게 영향을 미치는 것은 경사자계 유발 잡음이다. MRI와 동시에 측정한 뇌전도 신호에서 전류원 국지화를 수행할 때, 경사자계 유발 잡음이 미치는 영향을 분석하고자 한다. 대상 및 방법 : 사람의 머리와 유사하게 만든 뇌전도 팬텀과 MR compatible 뇌전도 측정시스템, 그리고 3.0 Tesla MRI 시스템을 실험에 사용하였다. 3.0 Tesla MRI 시스템 안에 전류원이 설치되어 있는 뇌전도 팬텀을 놓고, EPI 촬영을 하는 동안 뇌전도 신호를 측정하였다. 경사자계의 세기와 전류원의 위치를 조절하면서 뇌전도 신호 측정을 하였고, 측정된 뇌전도 신호에 대하여 전류원 국지화를 수행할 때 나타나는 국지화 오차를 평가하였다. 결과 : 경사자계 유발잡음에 의한 국지화 오차는 경사자계의 세기와 전류원의 위치에 따라 변화하는 것을 관찰하였다. 또한 경사자계 유발잡음이 Gaussian 잡음보다 전류원 국지화에 미치는 영향이 큰 것을 관찰하였으며, 경사자계 유발 잡음의 전력이 뇌전도 신호 전력의 $10\%$ 이하일 때는 전류원 국지화에 미치는 영향이 미미함을 관찰하였다. 결론 : 경사자계 유발 잡음 전력을 뇌전도 신호 전력의 $10\%$ 이하로 줄인다면 MRI를 하면서 동시에 측정한 뇌전도 신호로도 전류원 국지화를 할 수 있음을 알 수 있었다. Purpose : To evaluate the effect of the gradient switching noise on the ECD source localization with the EEG data recorded during the MRI scan. Materials and Methods : We have fabricated a spherical EEG phantom that emulates a human head on which multiple electrodes are attached. Inside the phantom, electric current dipole(ECD) sources are located to evaluate the source localization error. The EEG phantom was placed in the center of the whole-body 3.0 Tesla MRI magnet, and a sinusoidal current was fed to the ECD sources. With an MRI-compatible EEG measurement system, we recorded the multi channel electric potential signals during gradient echo single-shot EPI scans. To evaluate the effect of the gradient switching noise on the ECD source localization, we controlled the gradient noise level by changing the FOV of the EPI scan. With the measured potential signals, we have performed the ECD source localization. Results : The source localization error depends on the gradient switching noise level and the ECD source position. The gradient switching noise has much bigger negative effects on the source localization than the Gaussian noise. We have found that the ECD source localization works reasonably when the gradient switching noise power is smaller than $10\%$ of the EEG signal power. Conclusion : We think that the results of the present study can be used as a guideline to determine the degree of gradient switching noise suppression in EEG when the EEG data are to be used to enhance the performance of fMRI.
이성진(S . J . Lee),황규춘(K . C . Hwang),최강덕(K . D . Choi),이학교(H . K . Lee),신영수(Y . S . Shin),한재용(J . Y . Han) 한국축산학회 1995 한국축산학회지 Vol.37 No.3
This study was performed to analyze genetic characteristics of Korean Native Ogol Chickens compared with various breeds of chickens including Korean Native Yellow Chickens, White Leghorns and Rhode Island Reds. For DIVA fingerprinting, we collected blood samples from each of 10 individuals per breeds and obtained the genomic DNA. The genomic DNA samples were digested with restriction enzymes(Hinf I, Hcre III. etc.) and hybridized with various probes(Jeffreys` probes 33.15, 33.6, and M13) after Southern transfer. Probe 33.1 gave only a few unresolvable bands, however, probe 33.6 and M l3 detected a large number of bands and was thought to be the desirable probes for the analysis of genetic characteristics for various breeds of chickens. The use of pooled DNA enabled us to significantly reduce the number of DNA samples for estimation of genetic relationships among breeds. Furthermore, the results of DFP prepared from pooled DNA were similar to those of DFP prepared from individual DNA. In this analysis, KNOC was estimated to be a highly inbred line (band sharing $gt; 0.69), and to be closest in genetic distance to KNYC than any other bred.