누에 체성분이 생쥐의 혈액성분에 미치는 영향

강영국,추상규,남상호 한국곤충학회 2001 Entomological Research Vol.31 No.4

누에 체성분이 생쥐의 혈액성분에 미치는 영향을 조사하고자 5령 3일된 누에를 70% 알콜로 추출한 활성물질을 생쥐(C57BL/6)의 복강에 20mg/kg/day의 농도로 15일 동안 투여한 다음, 24시간 후에 실험동물을 $CO_2$로 회생시켜 체중변화와 혈액성분의 생화학적인 지표를 조사하였다. 그 결과, 체중과 혈중의 albumin, GOT, GPT 및 LDH의 함량은 누에추출물을 투여함에 따라 차이가 없었으나 creatinine, cholesterol, triglyceride, glucose, BUN 및 ALP의 함량은 감소하였다. To investigate the effects of silkworm-extract (SE) on the blood components levels in mice, we extracted the physiologically active substance by 70% ethanol from 5th instar 3rd day silkworm. Mice were administered intraperitoneally with the SE (20mg/kg/day) for 15 days. Animals were sacrificed with $CO_2$ gas 24 hr after the last SE treatment. Then, we investigated the body weight and biochemical markers on blood components levels in mice. The results, body weight and albumin, GOT, GPT, LDH of blood were not changed by the SE treatment to mice. But creatinine, cholesterol, triglyceride, glucose, BUN, ALP of blood were significantly decreased by the SE treatment to mice.

생체 내에서 Cholesterol이 간기능에 미치는 영향

김종호,윤기은,추상규 원광보건전문대 1992 원광보건전문대학논문집 Vol.15 No.-

금강유역(부여읍, 현북리)에 있어서의 간흡훼증의 역학적 조사

조경진,윤오섭,추상규,신현성 한국환경보건학회 1982 한국환경보건학회지 Vol.8 No.1

An epidemiological survey on 136 inhabitants of a Gum-River basin, Hyunbook-ri, Puyo-up, Puyo county, South ChoongCheong-Do, conducted from July 22, 1981 to July 25, 1981 with the purpose of studying Clonorchiasis, revealed the results as follows, 1. Positive skin reaction (wheal size larger than $60mm^2$) rate was 42% (55 positive reactors among the 131 studied), and the positive rates were all high at the age groups over twenty, while low at the below twenty. 2. In the stool examination, the employed cellophane thick smear method revealed the results as follows, overall infection rate of helminth: 65% (55 infected among 84 studied) - C. sinensis 33% (28/84), A. lumbricoides 5% (4/84), Taenia Sp 7% (6/84), T. trichiurus 32% (27/84). 3. The prevalence rate of C. sincrisis was highest in 40 - 49 year-old group by age: male 39% (11 infected/ 28 studied) female 14% (3/28), and not a single case was found in the lower age groups than twenty including school children.

호흡기 결핵 환자에서 임상 검사의 유용성

최우순 ( Woo Soon Choi ),추상규 ( Sang Kyu Choo ) 대한임상검사과학회 2006 대한임상검사과학회지(KJCLS) Vol.38 No.1

To find out the effectiveness of clinical examination for the diagnosis of respiratory tuberculosis, a 78 respiratory tuberculosis patient,s group was matched by sex and age with 78 control healthy subjects. In the result of blood chemistry, mean values of 123.5 ± 62.04 mg/dL in glucose, 429.01 ± 150.77 IU/L in LDH, and 44.51 ± 43.76 IU/L in γ-GTP, were higher than that of the controls (healthy subjects), and 3.51 ± 0.68 mg/dL in albumin was lower than that of the controls. In the result of the haematology examination, mean values of 12.52 ± 3.27 g/dL in hemoglobin, 36.72 ± 7.28% in hematocrit, and 24.61 ± 12.36% in lymphocyte, were lower than that of the controls, 9.23 ± 5.25% in monocyte 78.30 ± 37.35 mm/hr in ESR, and 48.45 ± 35.15 U/L in ADA were higher than that of the controls. For the comparisons of the tuberculosis patients values from normal reference values, 22.2% in glucose, 22.4% in LDH, 25.0% in γ-GTP, 35.4% in albumin, 88% in ESR, and 88.6% in ADA, showed abnormal values. We concluded that the values of glucose, γ -GTP, albumin, WBC, RBC, hemoglobin, hematocrit, lymphocyte, monocyte, ADA, and the ESR were useful in tuberculosis diagnosis.

검체의 보관방법과 시간 및 온도가 PT, aPTT 검사에 미치는 영향

김종석 ( Jong Suk Kim ),황석연 ( Seock Yeon Hwang ),추상규 ( Sang Kyu Chu ),김일택 ( Ill Taek Kim ) 대한임상검사과학회 2000 대한임상검사과학회지(KJCLS) Vol.32 No.3

We studied the stability of prothrombin time(PT) and activated partial thromboplastin time (aPTT) in 23 samples of healthy volunteers under different storage conditions. For this study, each blood sample was grouped into plasma and whole blood. πle temperatures forstorage of the plasma were at room temperature, at 4 degrees C and at -20 degrees C and the times for storage were from 0 hour up to 48hrs(at 0 , 4, 8, 16, 24 and 48 hours). πle whole blood was analysed under the same storage conditions as plasma except frozen storage condition. We defined that PT and aPTT were clinically stable if the percent(%) value of change was within 5 %. our data demonstrated that PT results of plasma was stable for up to 24hrs regardless of storage temperature and at both 4 degrees C and -20 degrees C stable for up to 48 hrs. PT results of whole blood was stable for up to 24 hrs at room temperature while at 4 degrees C the stability was less than 4hrs. on the other hand, aPTT of plasma was stable for up to 8 hrs either at room temperature or at 4 degrees C while for up to 48 hrs stable in freezing condition. Interestingly, We obserbed that aPTT stabililty of whole blood was no more than 4 hrs. From this study, it was found that the PT test was not easily affected by the temperature, storage time and the form of storage in comparison with the APTT test which was much easily affected by the above conditions. APTT should be done within 8 hours after blood was centrifuged immediately and the plasma should be stored freezing in order to obtain a reliable result.

항암제 투여한 골수성백혈병환자의 혈청단백 전기영동상

김종호 ( Chong Ho Kim ),장미경 ( Mi Kyoung Jang ),추상규 ( Sang Kyu Choo ) 대한임상검사과학회 2002 대한임상검사과학회지(KJCLS) Vol.34 No.2

In this present study, we carried out plasma protein electrophoresis by a method for cellulose acetate membrane electrophoresis and sodiumdodecylsulfate (SDS)-polyacrylamide gel electrophoresis (PAGE). Plasmas were isolated from patients with acute myelogenous leukemia (AML) and patients with chronic myelogenous leukemia (CML). The composition and variation of the electrophoreticprotein fractions were compared with those of normal patients. CML and AML patients showed a low concentration of albumin, 2-globulin, l-globulin and 2-globulin. The concentrations of 2-globulin and -globulin were high in CML plasma but low in AML plasma. l-globulin in the plasma of CML and AML patients was lower than normal. The results of the SDS-PAGE showed that the plasma isolated from AML and CML patients has a typical protein band (MW. 35 KD) and a thicker protein band (MW. 30 KD) than the protein band of normal patient``s plasma. These results suggested that it is possible to use these results for diagnosis of myelogenous leukemia. In addition, the two bands on SDS-PAGE make it interesting to study the possibility of the mechanisms of myelogenous leukemia.

임상화학 정도관리물질들의 안정성 평가

황석연 ( Seock Yeon Hwang ),김종석 ( Jong Seok Kim ),추상규 ( Sang Kyo Choo ),김일택 ( Il Teck Kim ),장철수 ( Cheoul Soo Chang ) 대한임상검사과학회 2000 대한임상검사과학회지(KJCLS) Vol.32 No.3

This study was carried out to evaluate the stability of commercial lyophllized control meterials being used for clinical chemical test and to investigate the substitutivity of a pooled serum prepared in our laboratory for those materials.Five sets of commercial lyophllized control meterials including normal and abnormal levels were obtained from five different manufacturers and stored under different conditions of storage(r∞m temp., refrigeration at 60C or freezing at 20C). πley were analyzed together with a pooled serum by Hitachl 7150 automated chemisσy analyzer and NOV A4 + electrolyte analyzer for the determination of 20 clinical chemical parameters and 3 electrolytes. The CV of within-batch, inter-batch and between-day in the precision analysis were below 1.3, 1.6 and 4.6%, respectively. It was revealed that total protein, albumin, creatinine, BUN, total cholesterol and TG were stable and their CV was less than 5% whlle total and direct bilirubin were unstable at room temperature and uφn light exposure with CV of 0.8 - 27.5 %. ALP at room temperature was also unstable. It was also revealed that pooled serum has no significant difference from those commercial control materials. The results above suggest that pooled serum could be used as a substitute for the commercial control materials with low cost.

급성심근경색증 진단에 대한 심근표지자와 생화학적 검사의 유용성

최우순 ( Woo Soon Choi ),김성철 ( Sung Chul Kim ),추상규 ( Sang Kyu Choo ) 대한임상검사과학회 2003 대한임상검사과학회지(KJCLS) Vol.35 No.1

To find the effectiveness of cardiac marker tests and blood chemistry analysis in the diagnosis of acute myocardial infarction, a 25 patient control group was matched by sex and age with 25 healthy subjects. Both groups were tested with cardiac markers and blood chemistry analysis was performed. The following are the results acquired from the study: There was a significant difference between the two groups in blood glucose, LDH, and CPK. There was a significant difference between the two groups in the cardiac marker, mean values of CK-MB, myoglobin, and troponin-T. The comparisons of the patients` blood chemistry values to normal reference values: 85.0% in LDH, 87.0% in myoglobin, 69.5% in CK-MB, 60.9% in troponin T, 45.2% in AST, 72.4% in CPK, 36.0% in glucose, and 24.3% in ALT show abnormalities. The results show diabetes in 20% of the acute myocardial infarction patients. Time series changes of positive values of CK-MB and troponin T were the highest at 100.0% from 12 to 24 hours. Troponin T positive value time series changes were the highest at 100.0% from 6 to 48 hours after acute myocadial infarction symptoms appeared.

북아메려카 접먼지진드기에 의한 알레르기 천식 환자에서 엄상검사의 유용성

최우순 ( Woo Soon Choi ),김성절 ( Sung Chul Kim ),추상규 ( Sang Km Choo ) 대한임상검사과학회 2005 대한임상검사과학회지(KJCLS) Vol.37 No.1

To find out the effectiveness of clinical examination for detection of dermatophagoides farinae allergen asthma disease, 50 patients (control group) and 50 healthy persons matched by sex and age to the control group, were tested for allergen asthma. The results of the study follow. The ESR there was significantly different from the comparisons of the patients`` normal reference values, 95.3 % in ESR, patient groups allergen asthma and heamatology values with control group. The ESR level of allergen asthma patients, 62.23 士35.09 mm/hr, was higher than that of the controls, 9.47 土5.36 mm/hr (p<O.OOI). The eosinophil count level of the patients, 370.65 土365.45 mm3, was higher than that of the controls with 171.99 土13 1.80 mm3 (p<0.001). πle IgE level of the patients, 1137.79 土784.69 IU/ml, was higher than that of the controls with 71.29 土14.28 IU/ml (p<0.001).