하나이 되게 하옵소서(요한17:11)

최화정 신학지남사 1960 신학지남 Vol.27 No.2

In Vitro Antiviral Activity of Sakuranetin against Human Rhinovirus 3

최화정 질병관리본부 2017 Osong Public Health and Research Persptectives Vol.8 No.6

Objectives: Rhinoviruses (RVs) cause common cold and are associated with exacerbation of chronic inflammatory respiratory diseases. Until now, no clinically effective antiviral chemotherapeutic agents to treat diseases caused by human rhinoviruses (HRVs) have been reported. We assessed the anti-HRV3 activity of sakuranetin isolated from Sorbus commixta Hedl. in human epithelioid carcinoma cervix (HeLa) cells, to evaluate its anti-rhinoviral potential in the clinical setting. Methods: Antiviral activity and cytotoxicity as well as the effect of sakuranetin on HRV3-induced cytopathic effects (CPEs) were evaluated using the sulforhodamine B (SRB) method using CPE reduction. The morphology of HRV3-infected cells was studied using a light microscope. Results: Sakuranetin actively inhibited HRV3 replication and exhibited antiviral activity of more than 67% without cytotoxicity in HeLa cells, at 100 μg/mL. Ribavirin showed anti-HRV3 activity similar to that of sakuranetin. Treatment of HRV-infected HeLa cells with sakuranetin visibly reduced CPEs. Conclusion: The inhibition of HRV production by sakuranetin is mainly due to its general antioxidant activity through inhibition of viral adsorption. Therefore, the antiviral activity of sakuranetin should be further investigated to elucidate its mode of action and prevent HRV3- mediated diseases in pathological conditions.

Evaluation of Antiviral Activity of Zanthoxylum Species Against Picornaviruses

최화정 질병관리본부 2016 Osong Public Health and Research Persptectives Vol.7 No.6

Human rhinoviruses and enteroviruses (family Picornaviridae) infect millions of people worldwide each year, but little is known about effective therapeutical treatment for the infection caused by these viruses. We sought to determine whether or not Zanthoxylum (Rutaceae) species can exhibit antiviral activity against picornaviruses. The leaf parts of four Zanthoxylum species were extracted with methanol, and the extracts were investigated for their antiviral activity against picornaviruses using cytopathic effects by cytopathic effect reduction. Leaf extracts of Zanthoxylum piperitum among four Zanthoxylum species were found to possess only broad-spectrum antipicornavirus activity against human rhninovirus 2 with a 50% inhibitory concentration (IC50) value of 59.48 ㎍/mL, human rhinovirus 3 with an IC50 value of 39.94 ㎍/mL, coxsackie A16 virus with an IC50 value of 45.80 ㎍/mL, coxsackie B3 virus with an IC50 value of 68.53 ㎍/mL, coxsackie B4 virus with an IC50 value of 93.58 ㎍/mL, and enterovirus 71 virus with an IC50 value of 4.48 ㎍/mL. However, ribavirin did not possess antiviral activity against human rhinovirus 3 and four enteroviruses. Therefore, leaves of Z. piperitum showed broad-spectrum antipicornavirus activity, and may be useful as a candidate for studying picornavirus agents and development of pharmaceuticals.

Application of PU/PPy Composite Film to Ionic Type Actuator Working in Air

최화정,정해도,이장우,조남주 한국공업화학회 2004 한국공업화학회 연구논문 초록집 Vol.2004 No.1

[연예인 데이트] 웬일이니? 그렇게 울 수가 있니! / [유머세계일주] 숨겨진 얼굴

최화정,편집부(편집자) 샘터사 1991 월간 샘터 Vol.22 No.7

The Role of Autonomous Wntless in Odontoblastic Differentiation of Mouse Dental Pulp Cells

최화정,고승오,조의식 대한치의학회 2016 Journal of korean dental science Vol.9 No.1

Purpose: Wnt signaling plays an essential role in the dental epithelium and mesenchyme during tooth morphogenesis. Deletion of the Wntless (Wls) gene in odontoblasts appears to reduce canonical Wnt activity, leading to inhibition of odontoblast maturation. However, it remains unclear if autonomous Wnt ligands are necessary for differentiation of dental pulp cells into odontoblast-like cells to induce reparative dentinogenesis, one of well-known feature of pulp repair to form tertiary dentin. Materials and Methods: To analyze the autonomous role of Wls for differentiation of dental pulp cells into odontoblast-like cells, we used primary dental pulp cells from unerupted molars of Wls-floxed allele mouse after infection with adenovirus for Cre recombinase expression to knockout the floxed Wls gene or control GFP expression. The differentiation of dental pulp cells into odontoblast-like cells was analyzed by quantitative real-time polymerase chain reaction. Result: Proliferation rate was significantly decreased in dental pulp cells with Cre expression for Wls knockout. The expression levels of Osterix (Osx), runt-related transcription factor 2 (Runx2), and nuclear factor I-C (Nfic) were all significantly decreased by 0.3-fold, 0.2-fold, and 0.3-fold respectively in dental pulp cells with Wls knockout. In addition, the expression levels of Bsp, Col1a1, Opn, and Alpl were significantly decreased by 0.7-fold, 0.3-fold, 0.8-fold, and 0.6-fold respectively in dental pulp cells with Wls knockout. Conclusion: Wnt ligands produced autonomously are necessary for proper proliferation and odontoblastic differentiation of mouse dental pulp cells toward further tertiary dentinogenesis.

바이러스와 치료제 : 곤충 및 호흡기 바이러스

최화정 한국응용곤충학회 2017 한국응용곤충학회 학술대회논문집 Vol.2017 No.10

바이러스로 인한 호흡기 질환은 가장 이환율이 높은 질병이며 미국의 통계 및 우리나라의 발생 추정치로 보아모든 감염질환 발생의 절반 정도를 차지하는 것으로 파악되고 있다. 사람의 호흡기 질환에는 많은 종류의 바이러스가직접 또는 간접적으로 관련되어 있으며 가장 잘 알려져 있는 인플루엔자 바이러스 외에 흔히 감기(common cold)를일으키는 병원체인 아데노바이러스(adenovirus), 파라인플루엔자 바이러스(parainfluenza virus, PIV), RS 바이러스(respiratory syncytial virus, RSV), 라이노바이러스(rhinovirus), 코로나바이러스(coronavirus)등이 대표적 병원체이다.또한 거미와 곤충, 벌레 등 척추가 없는 동물이 바이러스 산실이다. 곤충유래 무척추 동물들은 지카바이러스(zikavirus)나 뎅기바이러스(dengue virus) 등에 의한 감염질환을 일으키고 있다. 이러한 호흡기 및 곤충 바이러스들에대한 치료제들은 극히 일부 연구되어 상용화되고 있지만 내성 바이러스 출현으로 새로운 치료제 개발이 시급하다.따라서 다양한 식물체들을 활용한 바이러스 치료제 등에 관해 설명하고자 한다.

Saucernetin-8 from Schisandra chinensis as a Potent Antiviral Agent Against Coxsackievirus B3

최화정 대한미생물학회 2024 Journal of Bacteriology and Virology Vol.54 No.1

Various plants have been studied for their therapeutic potential to treat virusinduced diseases. This study aimed to investigate in vitro anti-coxsackievirus B3 (CVB3) effect of saucernetin-8 isolated from Schisandra chinensis and mode of its action. The action of saucernetin-8 on CVB3 proliferation, the effect of saucernetin-8 on VP3 protein, mitochondrial reactive oxygen species (mtROS) and expression levels of STING/p-STING/IRF3/p-IRF3 were investigated by RT-qPCR, immunofluorescence and Western blot. Saucernetin-8 exhibited over 60% inhibition of CVB3-induced cytotoxicity at a concentration of 0.4 μg/mL. Saucernetin-8 completely suppressed viral genome replication at a concentration of 10 μg/mL. Furthermore, saucernetin-8 inhibited the expression of VP3, the capsid protein of CVB3, by approximately 50% at a concentration of 10 μg/mL. Immunofluorescence assay showed that saucernetin-8 inhibited VP3 expression by approximately 50%. Saucernetin-8 increased mtROS levels in CVB3-infected Vero cells. The STING/IRF3 pathway is activated in cells treated with saucernetin-8. Therefore, saucernetin-8 is a candidate for development of antivirals against CVB3.

Inhibitory Effects of Norwogonin, Oroxylin A, and Mosloflavone on Enterovirus 71

최화정,송혁환,이재숙,고현정,송재형 한국응용약물학회 2016 Biomolecules & Therapeutics(구 응용약물학회지) Vol.24 No.5

Severe complications associated with EV71 infections are a common cause of neonatal death. Lack of effective therapeutic agents for these infections underlines the importance of research for the development of new antiviral compounds. In the present study, the anti-EV71 activity of norwogonin, oroxylin A, and mosloflavone from Scutellaria baicalensis Georgi was evaluated using a cytopathic effect (CPE) reduction method, which demonstrated that all three compounds possessed strong anti-EV71 activity and decreased the formation of visible CPEs. Norwogonin, oroxylin A, and mosloflavone also inhibited virus replication during the initial stage of virus infection, and they inhibited viral VP2 protein expression, thereby inhibiting viral capsid protein synthesis. However, ribavirin has a relatively weaker efficacy compared to the other drugs. Therefore, these findings provide important information that will aid in the utilization of norwogonin, oroxylin A, and mosloflavone for EV71 treatment.

Preparation and Characterization of Ionic Actuator based on Polypyrrole and Solid Polymer Electrolyte

최화정,박경미,정해도,이장우,조남주 한국고분자학회 2004 한국고분자학회 학술대회 연구논문 초록집 Vol.2004 No.04