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      • SCOPUSKCI등재

        ECAP 성형가공한 Al 2024 초미세결정립 재료의 소성변형량에 따른 강도 변화

        최정우,마영화,윤기봉,Choi, Jeong-Woo,Ma, Young-Wha,Yoon, Kee-Bong 대한기계학회 2005 大韓機械學會論文集A Vol.29 No.10

        Strength change of an over-aged A12024 material was studied after being subjected to stages of severe plastic deformation by ECAP (Equal Channel Angular Pressing). Various kinds of strength value were measured using the conventional tensile test, Rockwell and Vickers hardness and the SP (small punch) test Due to limitation of the specimen size, tension test in transverse direction could not be conducted. Hence, SP test was employed for assessing the strength in transverse direction. Based on TEM observation the measured strength characteristics were explained based on the relation between microstructure, dislocation and strength. As the number of ECAP pass increases, the strength of A12024 was also increased. However, considerable change of strength, which is generally predicted, was not observed in this study. For the strength in transverse direction even decrease of the strength was observed after 6 passes of ECAP. It was argued that this decrease was due to dynamic recovery of dislocation density during or after ECAP processes at $150^{\circ}C$. The strength assessment equation proposed by the authors in the previous paper was shown to be very accurate. This argument was supported by comparing the results of conventional tensile test with those of SP test. It was also pointed that the Rockwell har(3ness value seemed to be able to represent the strength in the transverse direction.

      • KCI등재후보

        Surface Plasmon Resonance Immunosensor for Detection of Legionella pneumophila

        최정우,이원홍,오병근,이우창,Young Min Bae 한국생물공학회 2003 Biotechnology and Bioprocess Engineering Vol.8 No.2

        An immunosensor based on surface plasmon resonance (SPR) onto a protein G layer by self-assembly technique was developed for detection of Legionella pneumophila. The protein G layer by self-assembly technique was fabricated on a gold (Au) surface by adsorbing the 11-mercaptoundecanoic acid (MUA) and an activation process for the chemical binding of the free amine (-NH2) of protein G and 11-(MUA) using 1-ethyl-3-(3-dimethylaminopropyl)carbodiimide hydrochloride (EDAC) in series. The formation of the protein G layer by self-assembly technique on the Au substrate and the binding of the antibody and antigen in series were confirmed by SPR spectroscopy. The surface topographies of the fabricated thin films on an Au substrate were also analyzed by using an atomic force microscope (AFM). Consequently, an immunosensor for the detection of L. pneumophila using SPR was developed with a detection limit of up to 102 CFU per mL.

      • KCI등재

        Fabrication of Protein A-Viologen Hetero Langmuir-Blodgett Film for Fluorescence Immunoassay

        최정우,Woochang Lee,Bum Suk Chun,Byung-Keun Oh,Won Hong Lee 한국생물공학회 2004 Biotechnology and Bioprocess Engineering Vol.9 No.4

        Protein A molecular thin film was fabricated as a platform of antibody-based biosensor. For the immobilization of the protein A thin film, a viologen multilayer was built up using the Langmuir-Blodgett (LB) technique, and then, protein A was adsorbed on the viologen LB film by an electrostatic interaction force, which was formed as a hetero-film structure. For the deposition of viologen, surface pressure area (-A) isotherm was investigated. The fabricated protein A-viologen hetero LB film was investigated using atomic force microscopy (AFM). Using the developed molecular film, antibody immobilization and fluorescence measurement was carried out.

      • KCI등재

        Nanoscale Fabrication of Biomolecular Layer and Its Application to Biodevices

        최정우,남윤석,마사미찌후지하라 한국생물공학회 2004 Biotechnology and Bioprocess Engineering Vol.9 No.2

        Biodevices composed of biomolecular layer have been developed in various fields such as medical diagnosis, pharmaceutical screening, electronic device, photonic device, environmental pollution detection device, and etc. The biomolecules such as protein, DNA and pigment, and cells have been used to construct the biodevices such as biomolecular diode, biostorage device, bioelectroluminescence device, protein chip, DNA chip, and cell chip. Substantial interest has focused upon thin film fabrication or the formation of biomaterials mono- or multi-layers on the solid surfaces to construct the biodevices. Based on the development of nanotechnology, nanoscale fabrication technology for biofilm has been emerged and applied to biodevices due to the various advantages such as high density immobilization and orientation control of immobilized biomolecules. This review described the nanoscale fabrication of biomolecular film and its application to bioelectronic devices and biochips.

      • KCI등재

        서울시 재산세 공동과세제도의 자치구 재정건전성 개선효과 분석: 광역시 자치구와의 비교를 중심으로

        최정우,이유현,배수호 한국지방자치학회 2012 韓國地方自治學會報 Vol.24 No.3

        Since 2008, Seoul has been operating the property tax-sharing system in order to ease the financial gap among 25 administrative districts. This system has contributed to mitigating financial imbalance among the administrative districts but it also has created revenue changes since property tax accounts for the largest portion of own revenue sources. These changes can affect administrative district's fiscal soundness. This study analyzes how the property tax-sharing system has affected fiscal soundness using a DID(difference in difference) method between administrative districts in Seoul and Six metropolitan cities. Here fiscal soundness is classified into the following three aspects:(1) the ratio of operating expenses in total expenses, (2) the ratio of own project/business expenses in total expenses, and (3) the ratio of own general revenues in total general revenues. For the purpose of comparing with Seoul's administrative districts, three comparison groups are employed as follows: (1) 15 administrative districts in Busan (2nd largest city in Korea), (2) 44 administrative districts in six metropolitan cities (excluding Seoul), and (3) 25 administrative districts selected among Since 2008, Seoul has been operating the property tax-sharing system in order to ease the financial gap among 25 administrative districts. This system has contributed to mitigating financial imbalance among the administrative districts but it also has created revenue changes since property tax accounts for the largest portion of own revenue sources. These changes can affect administrative district's fiscal soundness. This study analyzes how the property tax-sharing system has affected fiscal soundness using a DID(difference in difference) method between administrative districts in Seoul and Six metropolitan cities. Here fiscal soundness is classified into the following three aspects:(1) the ratio of operating expenses in total expenses, (2) the ratio of own project/business expenses in total expenses, and (3) the ratio of own general revenues in total general revenues. For the purpose of comparing with Seoul's administrative districts, three comparison groups are employed as follows: (1) 15 administrative districts in Busan (2nd largest city in Korea), (2) 44 administrative districts in six metropolitan cities (excluding Seoul), and (3) 25 administrative districts selected among the 44 administrative districts using a quasi-experimental design. According to DID results, the property tax-sharing system did not make any contribution to either an increase in the ratio of own project/business expenses in total expenses or an increase in the ratio of own general revenues in total general revenues. However, the system has contributed to an increase in the ratio of operating expenses in total expenses. As a conclusion, it can be interpreted that the system has promoted available resources and financial flexibility in administrative district's finance. 2008년부터 서울시는 자치구간 재정격차 완화를 위하여 자치구의 가장 큰 세원인 재산세를 대상으로 공동과세제도를 운영하고 있다. 본 제도는 자치구의 재정불균형 완화에 기여한다는 정책효과를 지니지만, 자치구의 세입원 중 가장 큰 비중을 차지하는 재산세를 대상으로 하므로 각 자치구의 세입변화를 야기하게 된다. 이러한 세입변화는 자치구 재정건전성을 변화시킬 수 있다. 따라서 본 연구는 재산세 공동과세제도가 재정건전성에 어떠한 영향을 주는지를 DID(difference in difference)방법론을 활용하여 서울시와 타 광역시 자치구와 재정건전성 비교분석을 실시하고자 한다. 재정건전성을 자체사업비율, 자체세입비율, 경상경비비율로 구분하여 비교분석하며 각각의 지표에 대해 부산시 자치구, 서울시를 제외한 5개 광역시 44개 자치구, 마지막으로 준실험설계(quasi-experimental design)를 통해 서울시 자치구와 유사한 재정규모를 지닌 25개 자치구를 선별하여 비교분석을 각각 실시한다. 분석결과, 공동과세제도는 자체사업비율, 자체세입비율에는 통계적으로 유의미한 영향을 주지 못하지만 경상경비비율에 있어서는 유의미한 영향을 주는 것으로 나타났다. 따라서 재산세 공동과세제도는 자치구 재정에 있어 가용자원의 증가와 재정의 탄력성 향상에 기여하고 있는 것으로 판단된다.

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