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최기봉,윤희주,차승희,최정도 ( Ki Bong Choi,Hee Ju Youn,Seung Hee Cha,Jung Do Choi ) 한국센서학회 1997 센서학회지 Vol.6 No.5
Fiber-optic evanescent wave sensor was designed and fabricated to detect mouse immunoglobulin G(IgG) with decladed optical fiber on which anti-mouse IgG was immobilized. A sensitivity obtained by any direct or competitive method was lower than 1 ㎍/㎖. Anti-mouse IgG was immobilized on 93.9% of core surface of optical fiber by simple adsoption method. The effect of postcoating using bovine serum albumin to remove non-specific binding was not observed. As the ratio of fluorescein to mouse IgG increased, the fluorescence signal increased, but that increase showed no linear relationship. Our fiber--optic sensor system could be used as immunosensor by measuring evanescent fluorescence in antigen-antibody reaction with good sensitivity below lug/mP level.