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최광수,박항균,서태광 경북대학교 유전공학연구소 1987 遺傳工學硏究所報 Vol.2 No.1
In order to obtain basic informations and to develop basic techniques for the production of monozygotic twins of Korean native goats, the study of superovulation, ova recovery, bisection of embryos and in vivo culture of separated blastomeres was carried out. For the study, 19 goats were treated with FSH to induce superovulation and, as a result, 16 goats were responded. Ova or embryos were recovered from the uterus by surgical method after 4.0-7.0 days from the first mating. The mean number of corpora lutea and recovered ova were 10.1±3.3 and 5.9±2.5, respectively. 53 morula were recovered after 5.0-6.0 days from the first mating. Of these recovered morula, 48 were bisected by glass needle or razor blade and 61 available demi-embryos were obtained among them. 61 available demi-embryos were cultured in vivo and after culture for 48 hours, low rate of the demi-embryos was recovered. However, some of the recovered demi-embryos were developed to blastocyst.