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대맥의 등숙일수와 수량구성요소와의 관계에 대한 유전연구 제I보 대맥의 생리적 성숙기 기준 설정
천종은,이은섭,이홍석,Chun,J.U.,Lee, E.S.,Lee, H.S. 한국작물학회 1982 한국작물학회지 Vol.27 No.1
Experiments were carried out to establish physiological maturity determination criteria with reference to visibly easy measurement in barley in 1980 at Suweon. Thirty-three cultivars and lines from 500 crossing blocks were classified into 4 heading groups, and 5 variables; moisture content, discoloration of awn, exsertion, lemma and flag leaf were measured. There were highly negative correlations between heading date and ripening periods (r=-0.656$^{**}$ ), so early heading types had longer ripening periods. Comparing with the variables used for maturity determination, moisture content and discoloration of lemma were most sensitive to development of grain-filling. Those two variables, alone or in combination could be used to screen many genotypes of barley for physiological maturity. In determination of maturity with reference to visibly easy measurement, color of lemma changed stably and was the most useful way and discolor of flag leaf increased the accuracy of determination. The color of lemma at this time was Grayish yellow, and the mean moisture content was about 33 percent in 33 barley cultivars and lines.
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PEG 6,000에 對한 麥酒보리 品種의 發芽率, 發芽勢 및 苗伸長 反應
千鍾殷,金東喆 順天大學校 農業科學硏究所 1991 農業科學硏究 Vol.5 No.-
Germination and seedling elongation of malting barley cultivars(Hangmaek, Sacheon6, Doosan8,12,22 and 29) were investigated at the six polyethylene glycol 6,000(PEG) solutions(0,100,150,200,250 and 300g/㎏ water). Adjustments of PEG solutions were based on the osmotic potentials of 0.0 - 0.166, -0.326, -0.534, -0.794 and -1.105 MPa at room temperature. The measurements were made at 2,3,4 and 9days after seeding. Fifty seeds of each cultivar were placed on Whatman No.1(9㎝) filter paper in plastic petri dishes, and added to 5-6㎖ of the proper PEG solutions under dark conditions. The germination rate of six malting barely cultivars did not decrease as PEG concentrations increased, with very excellent germination rate of all cultivars. However, the germination rate index(germination speed;GRI) markedly decreased below the osmotic potential of -0.326 MPa. The decrement rates of seedling length and fresh weight were 51% at PEG 150g/㎏ water and 30% at PEG 200g/㎏ water, respectively. The GRI and seedling length with increase of PEG concentrations follow almost the second degree polynomial and linear regression, respectively. The results of this study indicated that the measurements of GRI and seedling length of malting barley seeds in aqueous solutions of PEG would be very good indicator for screening cultivars for improved seed vigor during moisture stress.
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천종은,강석원 한국작물학회 2002 한국작물학회지 Vol.47 No.6
The combination of early heading time, maturing time and short grain-filling period is very important to develop early varieties in winter barley. The 4 parental half diallel crosses (parents, F₁s, F₂s) were cultivated at the field. The heading date was from April 3 to 26, maturing date from May 15 to 27 and grain-filling period from 31 days to 42 days, showing that the varietal differences about the 3 traits were remarkable. According to half diallel cross analyses, Dongbori 1 for heading time (late heading) was dominant, but Oweolbori (early heading) was recessive, showing partial dominance with high additive component of genetic variance. Dongbori 1 for maturing time was dominant, but Oweolbori was recessive, showing partial dominance with high additive variance. Reno for grain-filling period (short grain-filling period) was dominant, but Oweolbori (long grain-filling period) was recessive with additive, and partial dominance. There were highly significant mean squares for both GCA and SCA effects on the heading and maturing times, and GCA/SCA ratios for all traits were high, showing the additive gene effects more important. Sacheon 6 and Oweolbori had greater GCA effects for early heading and maturing times, and Dongbori 1 and Reno had greater GCA effects for late times. GCA effects were highly significant in F₁ and F₂ generations, showing high GCA/SCA ratios (7.02). The heading and maturing times in field were positively correlated with antifreeze proteins concentrations, accumulation, resistance to photoinhibition and winter survival, respectively) but the grain-filling period did negatively correlated with the trails. 보리의 조숙 품종을 육성하기 위해서는 조기 출수인자에 단기 등숙성 인자를 도입하는 것이 중요하므로 출수기, 성숙기 및 등숙기간의 유전에 관한 정보를 얻고자 4개 품종(사천6호, 오월보리, 동보리1호, Reno), F₁, F₂세대의 종자를 포장에 파종, 재배하여 출수기, 성숙기 및 등숙기간을 조사하였다. 1. 출수기는 4월 3일-4월 26일, 성숙기는 5월 15일-5월 27일, 등숙기간은 31일-42일로 교배친 및 F₁ 조합간 차이가 현저하였다. 2. 부분 이면교잡에 의한 분산분석 결과, 출수기에 대해서 동보리1호(출수기 늦음)는 우성으로, 오월보리는(출수기 빠름) 열성으로, 출수기 유전은 유전자의 상가적 작용이 큰 부분우성으로 나타났다. 3. 성숙기에 대해서 동보리 1호가 우성으로, 오월보리가 열성으로, 성숙기 유전은 유전자의 상가적 작용 큰 부분우성으로 나타났다. 4. 등숙기간에 대해서 Reno(등숙기간이 짧음)가 우성으로 오월보리(등숙기간이 길음)은 열성으로 유전자의 상가적 작용이 큰 부분우성으로 나타났다. 5. 출수기, 성숙기에 대한 일반조합능력의 분산량은 고도의 유의성이 인정되고, GCA분산이 SCA분산보다 출수기는 10.1-29.5배, 성숙기는 9.9-121배 높아서 유전자의 상가적 작용이 컸으며, 사천6호와 오월보리는 출수와 성숙이 빠른 방향으로, 동보리 1호와 Reno은 늦어지는 방향으로 효과를 보였다. 6. 등숙기간에 대해선 GCA 효과는 F₁과 F₂ 세대간 유의적 차이가 있었고 GCA/SCA비가 크므로 유전자의 상가적 작용이 현저하였다. 7 포장 출수기, 성숙기는 결빙방지 단백질의 농도, 광합성의 광저해에 대한 내성, 포장 내동성과 고도의 정의 상관을, 등숙기간은 각 형질들과 부의 상관을 보였다.
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천종은 한국차학회 2010 한국차학회지 Vol.16 No.3
The objective of the present work was to develop a rapid and accurate method to evaluate the quality and major chemical components of tea using near-infrared spectroscopy (NIRS). A total of 44 green tea samples were collected from the market places of several regions. The organoleptic quality of the green tea samples used in this work was assessed by professional tasters. The several chemical components were investigated by NIRS. Variations (based on CV = 2.4~4.0%) among the panelists that participated in the sensory test were relatively small, and differences in taste quality of the green tea samples were small. The ranking of taste quality was highly negatively correlated with the contents of vitamin C, total nitrogen, caffeine, theanine, and amino acids while negatively correlated with detergent fiber content. The powders of 44 processed green teas were scanned by NIRS with 1100~2500 nm bands in reflectance mode. The calibration equation for ranking of organoleptic quality was developed using the regression method of modified partial least squares (MPLS) with internal cross validation. The equations had low SECV (standard errors of cross-validation) and high R² (coefficient of determination in calibration) values of 0.963, indicating that NIRS could be used to rapidly and accurately evaluate the ranking of organoleptic quality in processed green teas.
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근적외선 분광광도계를 이용한 차 제품의 색상 및 카테킨류의 신속 측정
천종은 한국자원식물학회 2010 한국자원식물학회지 Vol.23 No.4
This study was done to measure the color and catechins contents in processed teas using the whole bands (400~2500 ㎚) with near-infrared spectroscopy(NIRS). The powder colors of 109 processed teas were measured with a colorimeter. The a/b ratios in Hunter color scale in processed teas accounted for about 98.9% of the variation in the fermentation degree(FD), indicating that the a/b ratio was a very useful trait for assessing fermentation degree. Also tea powders were scanned in the visible bands used with NIRSystem. The calibration equations for powder colors were developed using the regression method of modified partial least squares(MPLS) with the internal cross validation. The equations had low SECV (standard errors of cross-validation), and high R2 (coefficient of determination in calibration) values with 0.996~1.00, indicating that the visible bands(400~700 ㎚) with NIRS could be used to rapidly measure the variables related to powder color and fermentation degree. Also another powders of 137 processed teas were scanned at 780~2500 nm bands in the reflectance mode. The calibration equations were developed using the regression method of MPLS with the internal cross validation. The equations had low SECV, and high R2 (0.896~ 0.983) values, showing that NIRS could be used to rapidly discriminate the contents of EGC(R2=0.919), EC(0.896), EGCg(0.978), ECg(0.905) and total catechins(0.983) in processed teas with high precision and ease. 녹차, 부분발효차, 발효차 및 후발효차 등 다양한 차 제품 분말을 NIRSystem를 이용하여 전 대역(400-2500 ㎚)에서 스펙트럼을 얻어 중회귀분석에 의해 색상 관련 특성 및 카테킨류 성분에 대한 검량식을 유도하였다. Hunter color scale에서 발효정도의 변이를 a/b로 98.7% 설명될 수 있어 a/b값으로 차제품의 발효정도를 추정할 수 있다. MPLS를 이용하여 작성된 검량식의 결과 L값의 검량식 작성시 결정계수는 0.997, a값의 결정계수는 1.00, b값의 결정계수는 0.996, a/b값의 결정계수는 0.999로 매우 높았다. MPLS를 이용하여 작성된 검량식의 결과 EGC, EC, EGCg, ECg 및 총 카테킨의 결정계수는 각각 0.919, 0.896, 0.978, 0.905 및 0.983으로 매우 높았다. 차 제품 분말의 색상관련 특성들(L, a, b, a/b) 및 카테친류 (EGCg, EGC, EC, ECg 및 총 카테킨) 의 검량식 정확도가 매우 높아서 NIRS의 가시광선 대역(400~700 ㎚)및 근적외선 대역(780~2500 ㎚)에서 동시에 두 특성의 성분을 1~2분 내로 측정이 가능하다.
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