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차재호 서울大學校 社會科學大學 心理科學硏究所 1995 心理科學 Vol.4 No.1
파노라마적 사회심리학(차재호, 1991,1994)에서 사용되는 형태분석의 서설로서 재난에 대한 미국에서의 심리학적 재난연구를 중심으로 재난연구역사와 연구에서 밝혀진 결과들을 개관했다. 재난연구의 연혁, 재난의 구분, 재난과 사고의 관계, 그리고 재난 후의 심리사회적 영향, 재난전의 대응반응에 대해서 다루었다. 부분적으로 필자의 분석을 첨가했다. As a prelude to a planned paper on the configurational analysis of disasters within the framework of a panoramic social psychology proposed by the writer(Cha, 1991, 1994), social scientific studies dealing with disaters done in the United States were reviewed with special attention to topics of interest to psychologists. History of disaster studies, types of disasters, the relation between accident and disaster, psychological impact of disaster, psychological responses of survivors to disaster during and after the disaster, and psychological processes involved immediately prior to disaster impact including perception of danger and decision making in the face of impending danger were discussed. The writer's own analyses were added at appropriate places.
차재호 서울대학교 심리과학연구소 1988 심리학의 연구문제 Vol.- No.3
행위양가성과 결과불확정성이란 개념에 입각한 동기이론을 제시했다. 이 이론은 필자의 출산동기이론(Chung, Cha, & Lee, 1974)을 일반화시킨 것이다. 한 행동은 시도로서 정의하며 그런 시도는 2개의 상반되는 결과(예: 성공과 실패)를 내는 것으로 가정한다. 한 행위는 두 결과중 하나를 목적으로 하는 행동인데 그 행동은 두 결과 중 하나를 낳으나 어느 것이 될지는 행위자가 완전히 결정할 수 없다(결과불확정성). 각 행위 자체는 정적 및 부적 유인가를 모두 내포한다(행위의 양가성). 이 두 구조적 측면을 결합하면 4개의 칸 또는 4개의 요소동인이 나온다. 성취동기의 경우 이들 4요소는 성공희망, 성공공포, 실패희망, 및 실패공포이며 그 밖에 4요소동인설을 친애동기와 권력동기에 적용해 보았다. 4요소동인설은 좌절, 갈등 그 밖에 인간동기에 관한 연구결과를 통합하는 개념적인 틀을 제공하고 새로운 시각을 제공한다.
Stromelysin-1에 의한 펩타이드 가수분해에서 pH와 기질특이성 연구
차재호,Marianne V. Sorensen 한국생명과학회 2000 생명과학회지 Vol.10 No.2
A kinetic profile of the catalytic domain of stromelysin-1 (SCD) using the fluorescent peptide substrate has been determined by the stopped-flow technique. The pH profile has a pH optimum of about 5.5 with an extended shoulder above pH 7. Three pKa values, 5.0, 5.7, and 9.8 are found for the free enzyme state and two pH independent Kcat/Km values of 4.1$\times$104 M-1 s-1 and 1.4$\times$104 M-1 s-1 at low and high pH, respectively. The profile is quite different in shape with other MMP family which has been reported, having broad pH optimum with two pKa values. The substrate specificity of SCD towards fluorescent heptapeptide substrates has been also examined by thin layer chromatography. The cleavage sites of the substrates have been identified using reverse-phase HPLC method.SCD cleaves Dns-PLA↓L↓WAR and Dns-PLA↓L↓FAR at two positions. However, the Dns-PLA↓LRAR, Dns-PLE↓LFAR, adn Dns-PLSar↓LFAR are cleaved exclusively at one bond. The double cleavages of Dns-PLALWAR and Dns-PLALFAR by SCD are in marked contrast to the close structurally related matrilysin. A notable feature of SCD catalysis agrees with the structural data that the S1' pocket of SCD is deeper than that of matriysin. The differences observed between SCD and matrilysin may form the basis of understanding the structural relationships and substrate specificities of the MMP family in vivo.
V. parahaemolyticus 에 의한 Alkaline Protease 생산조건 (1)
차재호,양지영,황미경,강현록,한종흔 한국식품위생안전성학회 2000 한국식품위생안전성학회지 Vol.15 No.2
V. parahaemolyticus possessed an extracellular alkaline protease activity during the stationary growth phase. Various factors such as nitrogen sources, the concentration of NaCl and metal ions were investigated for optimizing the production of alkaline protease from V. parahaemolyticus ATCC 17802. Among the nitrogen sources tested skim milk showed the distinct increase of the activity and the activity was the highest at 2% in final concentration after 60 hours incubation. The addition of NaCl and metal ions did not increase the alkaline protease activity. CoC1₂, CuC1₂, and HgCl rather highly inhibited alkaline protease production.
뼈형성 단백질(Bone Morphogenetic Protein 1)의 단백질 분해 부위의 발현 및 특성 연구
차재호,Daihung Do 한국생명과학회 2000 생명과학회지 Vol.10 No.2
Bone morphogenetic protein 1 (BMP-1) is part of a complex capable of inducing ectopic bone formation in mammals. Studies on TGF-β1 processing and Drosophila dorsal-ventral patterning have focused attention on BMP-1 as important in mediating the biological activity of this bone inducing complex. Herein, the bacterial expression, refolding, purification, and initial characterization of the BMP-1 proteolytic domain (BPD) are described. A semi-quantitative fluorescence-based thin layer chromatography assay was developed to assist in rapidly screening for optimal renaturation conditions. According to a preliminary screen for optimal conditions for the refolding of BPD , a detectable proteolytic activity against a high turnover substrate for astacin, a homologous protease from crayfish was observed. The conditions identified have allowed the expression of sufficient amounts of BPD for the characterization of the protein. Its proteolytic activity exhibits the same cleavage specificity as astacin against seven substrates that were previously synthesized for studying astacin. Furthermore, this activity is inhibited by the metal chelator 1,10-phenanthroline but not by its analogue 1,7-phenanthroline. The collagenase inhibitor Pro-Leu-Gly hydroxamate was found to inhibit both astacin and BPD activity. The results presented in this paper argue that BMP-1 does in fact possess an intrinsic proteolytic activity.