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Indomethacin으로 유발된 생쥐의 위점막 손상에 대한 쑥을 함유한 천연물 복합제의 효과
정성연 ( Seong-yeon ),주인환 ( Jeong In-hwan Joo ),박종민 ( Jong-min Park ) 대전대학교 한의학연구소 2022 혜화의학회지 Vol.32 No.1
Objectives : This study was aimed to investigate whether gastritis care mixture (GCM) with artemisi a can prevent indomethacin-induced gastric damages. GCM consisted of artemisiae argyi folium, diosc oreae rhizoma, crataegi fructus, zizyphi fructus, and glycyrrhizae radix. Methods : Antioxidant activities were evaluated using 2,2-dipheny-1-picrylhydrazyl (DPPH), 2,2'-a zinobis-(3-ethylbenzothiazoline-6-sulfonic acid (ABTS), and ferric reducing antioxidant power (FRA P) method. GCM were pretreated 6 h before indomethacin treatment on rat gastricmucosal epithelial BGM1 cells. Cytoprotection effects eavluated by MTT. [3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide]. Reverse-transcription PCR (RT-PCR) and Western blot was performed to check anti-inflammatory actions. Different doses of GCM were administrated intragastrically before the indomethacin administration in mouse models. After killing, addition to gross and pathological evaluation of ulcer, the expressions of inflammatory mediators were analyzed by enzyme-linked immunosorbent assay (ELISA). Results : GCM was very effective in preventing indomethacin-induced gastric damages in a low dose. indomethacin increased the expression of cyclooxygenase-2 (COX-2) and decreased the expression of 15-hydroxyprostaglandin dehydrogenase (15-PGDH) and Heme oxygenase-1 (HO-1), but GCM significantly attenuated indomethacin-induced COX-2 expression. Interestingly, GCM induced expression of 15-PGDH and HO-1. These in vitro findings were exactly validated in indomethacin-induced gastritis mouse models. GCM groups showed significantly ameliorated patheologic lesion compared to the control group. The serum levels of proinflammatory cytokines including interleukin-6 and tumor necrosis factor were significantly attenuated in the GCM group. However, these preventive effects of G CM were dependent on dosage of GCM; higher dose above 200mg/kg paradoxically aggravated indomethacin-induced inflammation. Conclusions : GCM is a candidate substance which can attenuate NSAIDs-induced gastritis on potent anti-inflammatory action.
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