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조현화,김규 진주산업대학교 2000 論文集 Vol.39 No.-
This study is attempted to provide information that will be useful to the private managers of collective houses who intend to improve the collective house management. The objective of the study was an analytical survey on the improvement of the collective house management that would make every occupants' lives more convenient and safe, and minimize the costs of housing management. For this purpose a total of occupants of apartments in Chinju were put to questionnaire. The several facts that would make housing management improved were obtained from the survey. The alternatives for the effective management of the collective house were showed as follows : First, the effective management of the collective house has mainly been dependent on the laws and institutions related to it, and how to manage it such as flaw repair, maintenance, safety management, and so on. Second, if housing management is to be effective related laws are systematically and rationally improved. Laws and regulations have taken a negative effects on housing management. The law requires that adequate measure be devised if government concerned implement regulations that can influence the housing management. The occupants may manage the collective house for themselves instead of managers being licensed by the government to conduct their management. The laws and regulations suitable for direct management in actual must be revised and acted. These changes have improved the their existing system, and come into effect on the housing management. Third, there is no doubt that managers' ways of thinking and values have great influence on the housing management. There should, therefore, be established a institution so that the mangers may learn how to manage the housing management in a more professional way. Forth, if housing management is to be effective a comprehensive, positive participation of occupants is necessary. Occupants should maintain a high level of interest in housing management. Meaningful cooperative behaviors, from which every occupants could benefit, should be planned and pursued as well. Occupants's behaviors beneficial for housing management in actual are expected to produce significant results.
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인체혈청 하에서 배양한 인체지방기질줄기세포의 표면항원 및 유전자 발현
전은숙,조현화,주혜준,김회규,배용찬,정진섭,Jun, Eun-Sook,Cho, Hyun-Hwa,Joo, Hye-Joon,Kim, Hoe-Kyu,Bae, Yong-Chan,Jung, Jin-Sup 한국생명과학회 2007 생명과학회지 Vol.17 No.5
인간중간엽줄기세포는(Human mesenchymal stem cells, hMSC) 골수, 지방, 피부, 근육, 혈액에 존재하며, 뼈, 연골, 지방, 근육, 신경세포로 분화가능성이 보고되어 손상조직의 재생을 위한 재료로서뿐만 아니라 유전자치료의 매개체로 이용될 수 있는 가능성이 제안되고 있다. 인간중간엽줄기세포의 적절한 배양조건에는 소 태아혈청(fetal bovine serum, FBS)이 요구되어지므로 세포치료에는 소 태아혈청이 다수 포함되어 있을 것이며 세포배양 배지 유래 소 태아혈청의 단백질에 의한 면역거부반응이 우려된다. 이미 앞선 연구에서 자가혈청 하에서 인체지방줄기세포 분리와 계속적인 세포배양을 실시하였을 때 인체지방줄기세포의 증식능력과 다 분화 능이 유지되며 면역결핍 생쥐에 골수의 말초혈액에서 유래된 CD34세포 이식 시 안착 능을 촉진함을 보였다. 본 연구에서 인체지방줄기세포가 인체혈청 하에서 배양되었을 때 소 태아혈청 하에서 배양할 때 발현하는 표면항원을 유지함을 확인했으며 microarray를 사용하여 유전자 발현을 비교했다. 유 세포 분석을 통하여 인체혈청 하에서 계속적으로 배양된 인체유래지방줄기세포에서 HLA-DR, CD117, CD29 와 CD44 의 발현이 소 태아혈청 하에서 배양했을 때와 비슷함을 밝혔다. 그러나 인체혈청 하에서 배양된 인체지방줄기세포의 유전자 발현형태와 소 태아혈청 하에서 배양된 세포의 유전자 발현형태 간에는 상당한 차이를 보였다. 그러므로 본 연구는 인체혈청 하에서 배양된 인체지방기질줄기세포가 임상적용을 위한 선행 데이터로써 직접적인 추정을 하기 위해서는 인체지방기질줄기세포 이식연구에 in vivo 동물실험연구가 수행되어져야 함을 제시하고 있다. Human mesenchymal stem cells(hMSC), that have been reported to be present in bone marrow, adipose tissues, dermis, muscles and peripheral blood, have the potential to differentiate along different lineages including those forming bone, cartilage, fat, muscle and neuron. Therefore, hMSC are attractive candidates for cell and gene therapy. The optimal conditions for hMSC expansion require medium supplemented with fetal bovine serum(FBS). Some forms of cell therapy will involve multiple doses, raising a concern over immunological reactions caused by medium-derived FBS proteins. Previously, we have shown that hADSC can be cultured in human serum(HS) during their isolation and expansion, and that they maintain their proliferative capacity and ability for multilineage differentiation and promote engraftment of peripheral blood-derived CD34 cells mobilized from bone marrow in NOD/SCID mice. In this study we determined whether hADSC grown in HS maintain surface markers expression similar with cells grown in FBS during culture expansion and compared gene expression profile by Affymetrix microarray. Flow cytometry analysis showed that HLA-DR, CD117, CD29 and CD44 expression in HS-cultured hADSC during culture expansion were similar with that in FBS-cultured cells. However, the gene expression profile in HS-cultured hADSC was significantly different from that in FBS-cultured cells. Therefore, these data indicated that HS-cultured hADSC should be used in vivo animal study of hADSC transplantation for direct extrapolation of preclinical data into clinical application.
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