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쓴메밀 및 단메밀 에탄올 추출물의 항산화 및 지방세포 분화억제 효과
윤보라 ( Bo Ra Yoon ),조봉제 ( Bong Jae Cho ),이효구 ( Hyo Ku Lee ),김대중 ( Dae Jung Kim ),이성갑 ( Seong Kap Rhee ),홍희도 ( Hee Do Hong ),김경탁 ( Kyung Tack Kim ),조장원 ( Chang Won Cho ),최현선 ( Hyeon Son Choi ),이부용 ( Boo 한국식품저장유통학회 ( 구 한국농산물저장유통학회 ) 2012 한국식품저장유통학회지 Vol.19 No.1
In this study, 80% ethanolic extracts of tartary and common buckwheats were assessed for their total phenol content, total flavonoids content, antioxidant activity (DPPH, ABTS radical scavenging activity and reducing power), and anti-adipogenic effects. Our results show that total phenol contents of 80% ethanolic extract from tartary and common buckwheats were 17.35±0.41 and 8.20±0.28 μg GAE/g, respectively. Antioxidant activities of 80% ethanolic extract from tartary buckwheat were significantly higher than that of common buckwheat extract (p<0.05). During adipocyte differentiation, 80% ethanolic extracts of tartary and common buckwheat significantly inhibited lipid accumulation compared to control cells. We further evaluated the effect of buckwheat extracts on the changes of key gene expression associated with 3T3-L1 adipogenesis and ROS production. Tartary buckwheat extract was more suppressed the mRNA expressions (PPARγ and aP2) than that of common buckwheat extract. Moreover, tartary buckwheat inhibited the mRNA expression of both NOX4 (NADPH oxidase 4) and G6PDH (glucose-6-phosphate dehydrogenase). These results indicate that anti-adipogenesis effect of tartary buckwheat can be attributed to phenolic compound that may potentially inhibit ROS production.