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Alcaligenes faecalis NS13에 의한 호기성 종속영양 질산화 및 탈질화
정택경,라창식,조기성,송홍규,Jung, Taeck-Kyung,Ra, Chang-Six,Joh, Ki-Seong,Song, Hong-Gyu 한국미생물학회 2016 미생물학회지 Vol.52 No.2
In order to find an efficient bacterial strain that can carry out nitrification and denitrification simultaneously, we isolated many heterotrophic nitrifying bacteria from wastewater treatment plant. One of isolates NS13 showed high removal rate of ammonium and was identified as Alcaligenes faecalis by analysis of its 16S rDNA sequence, carbon source utilization and fatty acids composition. This bacterium could remove over 99% of ammonium in a heterotrophic medium containing 140 mg/L of ammonium at pH 6-9, $25-37^{\circ}C$ and 0-4% of salt concentrations within 2 days. It showed even higher ammonium removal at higher initial ammonium concentration in the medium. A. faecalis NS13 could also reduce nitrate and nitrous oxide by nitrate reductase and nitrous oxide reductase, respectively, which was confirmed by detection of nitrate reductase gene, napA, and nitrous oxide reducase gene, nosZ, by PCR. One of metabolic intermediate of denitrification, $N_2O$ was detected from headspace of bacterial culture. Based on analysis of all nitrogen compounds in the bacterial culture, 42.8% of initial nitrogen seemed to be lost as nitrogen gas, and 46.4% of nitrogen was assimilated into bacterial biomass which can be removed as sludge in treatment processes. This bacterium was speculated to perform heterotrophic nitrification and aerobic denitrification simultaneously, and may be utilized for N removal in wastewater treatment processes.
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돼지위축성비염과 가금티푸스 병원균을 저해하는 Bacillus amyloliquefaciens TK3의 분리 및 특성 조사
정택경,송홍규,Jung, Taeck-Kyung,Song, Hong-Gyu 한국미생물학회 2015 미생물학회지 Vol.51 No.2
For prevention of atrophic rhinitis of swine by Bordetella bronchiseptica and fowl typhoid by Salmonella gallinarum, bacterial strains showing antimicrobial activity against those pathogenic bacteria were isolated from various samples collected at animal farms. Among 372 bacterial isolates strain TK3 showed the highest antibacterial activity against both pathogens, and was identified as Bacillus amyloliquefaciens by 16S rRNA gene sequence analysis. B. amyloliquefaciens TK3 could inhibit growth of both pathogens by secretion of antibacterial compounds such as siderophore, rhamnolipid and antimicrobial peptide. Production radius of siderophore on Chrome azurol S agar plate by strain TK3 was 0.53 cm after 14 days of incubation, and concentration of siderophore in King's B medium was 1.06 mmol/ml. It also secreted 82.4 mg/L of rhamnolipid, and antimicrobial peptide that completely inhibited growth of both pathogens at concentration of $30{\mu}l/ml$ in LB medium.
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식물병원성 진균을 억제하는 근권세균의 항진균능과 식물생장촉진능
정택경,김지현,송홍규,Jung, Taeck-Kyung,Kim, Ji-Hyun,Song, Hong-Gyu 한국미생물학회 2012 미생물학회지 Vol.48 No.1
Since many pesticides cause various health and environmental problems, alternative measures to replace them are needed, and the bacteria producing the antifungal substances can be one of them. In this study, several rhizobacteria were isolated and their antifungal activities against some important plant pathogenic fungi were examined. Pseudomonas otitidis TK1 and Paenibacillus peoriae RhAn32 inhibited the growth of Fusarium oxysporum f. sp. niveum and F. oxysporum f. sp. lycopersici by 49.8% and 45.6%, and 45.1% and 48.3%, respectively compared to those of the control. P. peoriae RhAn32 also decreased the growth of F. oxysporum f. sp. raphani by 37.5%. This growth inhibition might be due to the production of antifungal substances, such as siderophore, hydrogen cyanide and chitinase, which were produced by these rhizobacteria. P. otitidis TK1 also produced plant growth hormones indole acetic acid and indole butyric acid at $293.41{\mu}g/mg$ protein and $418.53{\mu}g/mg$ protein, respectively. When P. otitidis TK1 and B. cereus TK2 were inoculated together with F. oxysporum f. sp. lycopersici to the 4 weeks grown tomato seedlings and incubated additional 8 weeks, the stem lengths of tomato increased up to 45.7% and 55.3% and root lengths were raised to 64.9% and 60.8%, respectively than those of the control group. The wet weights increased by 118% and 182%, respectively compared to the control group.
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이자와 고이치(井澤耕一),서민교(번역자) 한국국학진흥원 2010 국학연구 Vol.16 No.-
본 논고는 ‘제가齊家의 예’를 논한 ??가례??가 아니라 그 다음 단계인 ‘치국의 예’를 논한 ??주례??에 주로 초점을 두고 남송기의 ??주례?? 해석을 주희의 시점을 중심으로 고찰한 것이다. 그 결과 주희는 ??주례??가 주공周公이 구상을 짜내고 관리들에 의해서 집필된 예에 관한 ‘강령綱領’이라고 간주하고 있다는 것, 또 ??주례??를 해석할때에는 왕안석王安石과 진전량陳傳良과 같이 자기의 학설, 즉 ‘사의私意’에 의해서 해독하는 것이 아니라 ‘차요취절실리회수용처且要取切實利會受用處’ 즉 우선은 절실하게 대응할 수 있는 것에서부터 시작해야 한다고 강조하고 있었다는 것이 밝혀졌다.
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