천연물 유래의 5α-Reductase 저해제의 개발과 인체 유래 피지선 세포의 배양을 이용한 피지분비 억제 효과 측정

정세규 ( Se Kyoo Jeong ),김정기 ( Jeong Kee Kim ),백지훈 ( Jihwoon Baek ),이기무 ( Ki Moo Lee ),조인식 ( In Shik Cho ),이승훈 ( Seung Hun Lee ) 대한화장품학회 2005 대한화장품학회지 Vol.31 No.3

본 연구에서는 피지분비 억제 효과를 지닌 신규 화장품 원료를 대한민국 유래의 천연 식물에서 개발하고자 하였다. 다양한 식물 추출물의 5alpha-reductase에 대한 저해효과를 자외선 분광광도계를 이용한 효소 동력학적 분석법을 통하여 평가하였다. 효소 동력학적 분석을 위하여 각각 쥐의 간 조직과 배양된 인체 유래 피지선 세포에서 5alpha-reductase를 추출하였다. 그 결과, 5-AR에 대하여 뛰어난 저해효능을 보이는 3종의 식물 추출물을 선정하였고, 이들의 피지분비 억제 효과를 배양된 인체 유래 피지선 세포를 이용하여 분석하였다. 연구의 결과, 수십 종의 식물 추출물 중 해송 추출물이 가장 강력한 5-AR 저해능을 나타내었으며, 인체 유래 피지선 세포에 대하여 0.005% 농도로 처리한 경우 48%의 피지 생성량 감소 효과를 나타내었다. 해송 추출물은 피지 분비를 감소시켜 여드름이나 지루성 피부염과 같은 피부 질환에 효과를 지닌 화장품의 원료로 사용될 수 있을 것이다. This study was performed to develop new cosmeceutical agents with sebosuppressive activity from native plant extracts in Korea. Inhibitory efforts of the extracts on 5α-reductase (5-AR) were evaluated by enzyme kinetics analysis using UV-spectrophotometric method. Two kinds of enzyme suspensions as 5-AR sources were prepared from rat liver tissue and cultured hSG cells. The sebosuppressive effects were determined by measuring the total lipid quantity produced in cultured hSG cells after incubation with the extracts. As a result, Pinus thunbergii extracts showed the most potent 5-AR inhibitory effects. Its Ki values were 0.0002% and 0.0014% for rat liver 5-AR and human sebaceous gland 5-AR, respectively. Addition of Pinus thunberii extract to hSG cells showed 48% reduction in total lipid production at 0.005% concentration. In conclusion, Pinus thunbergii extracts can be used as a cosmeceutical agent to regulate sebum production and to alleviate the sebum-involved skin diseases, such as acne and seborrheic dermatitis.

Development of moisturizers

정세규 ( Se Kyoo Jeong ),박병덕 ( Byeong Deog Park ),이승헌 ( Seung Hun Lee ) 한국피부장벽학회 2007 한국피부장벽학회지 Vol.9 No.1

The use of topically applied materials to improve appearance, function, and feel of skin is as old as human life. Our knowledge of the mechanisms by which all of these agents work continues to evolve along with our understanding of skin structure and function, especially that of the stratum corneum (SC). However, even with the enormous efforts performed so far, the exact mechanisms are not yet totally understood. The term moisturizer implies to substances, that applied to skin, help adding water and/or retaining water in the SC. Diverse materials, ranging from natural moisturizing factors in the SC to ceramide as a structural constituent of SC intercellular lipids, are currently used as moisturizing agents but the complete mechanism of these moisturizing agents is not yet fully elucidated. Recent advances in the area of skin research show that various skin functions are closely interrelated with skin hydration. Altered epidermal permeability barrier function can result from abnormal skin hydration and vice versa. Terminal differentiation of epidermal keratinocyte provides the natural moisturizing factors in SC, which play a crucial role for retaining the water molecules in SC. These observations suggest a new strategy for developing a good moisturizer, and make it more important to study the basic mechanisms of skin hydration. Sufficient understanding of the formulation of moisturizers and their properties as cosmetic products is also needed for the development of a good moisturizer. In this article, recent advances in basic research and practical development of moisturizers are discussed.

피부장벽과 각질세포간 지질

정세규 ( Se Kyoo Jeong ),김현정,박병덕,이승헌 한국피부장벽학회 2009 한국피부장벽학회지 Vol.11 No.1

피부는 인체가 생존하는 데 있어 필수적인 다양한 기능을 수행하고 있다. 환경 변화에 대응하여 인체 내부의 항상성을 유지하기 위한 장벽 기능, 외부 변화를 인지하기 위한 감각 기능, 체온 조절 기능 등이 가장 대표적인 피부의 기능에 속하며 이들은 모두 외부 환경의 변화에 대응하여 인체 내부의 기능을 유지할 수 있는 항상성을 유지하는 역할을 한다. 다양한 피부의 기능 중, 특히 피부의 장벽 기능은 주로 피부의 가장 외각에 위치하는 각질층에 의해 나타나게 된다. 그러나, 최근의 연구 결과에 따르면, 각질층은 단순한 장벽 기능뿐 아니라 내부의 살아있는 세포층, 즉 표피층이나 진피층의 기능 및 역할, 구조 등에도 영향을 미치는 것으로 보고된 바 있어, 그 중요성이 지속적으로 증가하고 있다. 각질층의 중요성이 강조됨에 따라 이에 대한 연구가 활발하게 이루어지고 있으며, 특히 각질층 내에 존재하는 각질세포간 지질의 구조와 기능 등에 대한 연구도 매우 활발하게 이루어지고 있다. 많은 수의 피부 질환에 있어서 피부 장벽 기능의 이상이 동반되거나, 주요한 병인으로 인식되고 있으며 특히 각질세포간 지질의 성분 변화에 따른 구조적인 변형 등이 중요한 것으로 알려지게 되면서, 지질에 대한 연구가 활발하게 이루어지고 있다. 각질세포간 지질은 라멜라 구조라 불리는 여러 층의 지질막 형태를 나타내고 있으며, 특히 세라마이드 성분이 각질세포간 지질의 기능에 중요한 역할을 하는 것으로 알려지면서 이를 근거로 하여, 다양한 보습제 등이 개발되고 있다. 피부의 장벽 기능이 손상되면, 지질의 분비와 합성 등을 포함하는 다양한 항상성 유지 반응의 나타나게 되며, 피부장벽 기능이 손상된 피부에 각질세포간 지질의 성분을 함유하고 있는 외용 제제를 도포하게 되면 피부장벽 기능을 회복하는 등의 효과를 나타내는 것으로 알려져 있다. 이러한 이론적 근거를 바탕으로 하여 아토피 피부염이나 건선 등의 피부질환에 사용되는 보습제를 개발하는데 있어서 각질세포간 지질 성분을 이용하고자 하는 연구가 꾸준히 이루어져 왔으며, 최근의 임상 시험 결과 등에 따르면, 특히 아토피 피부염에 있어서는 각질세포간 지질 성분으로 구성된 보습제가 외용 스테로이드제제와 유사한 임상 효능을 가지는 것으로 보고된 바 있다. 본 고에서는 이러한 각질세포간 지질의 구성과 구조, 장벽 기능에 미치는 영향 등에 대하여 최근의 연구 결과를 바탕으로 간략히 소개하고자 한다.

화학적 박피가 피부장벽에 미치는 영향에 대한 비교연구

정세규(Se Kyoo Jeong),김산(San Kim),이은희(Eun Hee Lee),최응호(Eung Ho Choi),안성구(Sung Ku Ahn),이승헌(Seung Hun Lee) 대한피부과학회 2002 대한피부과학회지 Vol.40 No.10

N/A Background : Among the various methods for chemical peeling, it is possible to select a wide range of peeling agents for particular patients. Objectives : The objective of present study was to investigate the effects of various chemical peeling agents on the epidermal permeability barrier of hairless mice skin and to clarify the histologic alteration in epidermal structure, thus to apply in the clinical practices. Methods : We have applied 35% and 70% glycolic acid (GA) aqueous solutions, 30% of salicylic acid (SA) solution of PEG400, Jessner`s solution and 15%, 30% and 50% of trichloroacetic acid (TCA) aqueous solution to the flank of hairless mice. TEWL (trans-epidermal water loss) values were measured before and immediately after the application and 3, 6, 12 and 24 hours following treatment. Biopsy specimens were evaluated with ligth and electron microscopy for epidermal structural changes. Results : There were no signific in TEWL for the GA and SA solution treated skin, regardless of their concentration. For the TCA and Jessner`s solution, TEWL increased immediately after treatment and recovered the basal levels about 90% after 24 hours for Jessner`s solution and low concentrated TCA solution, but did not recovered for high concentrated TCA solution. On light and electron microscopic examination, exfoliating effect was seen in every case and as for SA and Jessner`s solution treated skin, keratolysis at hair follicles was also seen. Slight epidermal necrosis was seen in every case, except in GA treated skin. Conclusion : The present results suggest that using topical age is such as glycolic acid can induce the change in the architecture of the epidermis without disrupting the skin barrier.

스트론튬 이온이 무모 생쥐의 표피 투과 장벽 회복에 미치는 영향

김현정 ( Hyun Jeong Kim ),김민정 ( Min Jung Kim ),정세규 ( Se Kyoo Jeong ),최기주 ( Ki Ju Choi ),서정택 ( Jeong Taek Seo ),최응호 ( Eung Ho Choi ),안성구 ( Sung Ku Ahn ),이승헌 ( Sung Hun Lee ) 대한피부과학회 2006 대한피부과학회지 Vol.44 No.11

Background: Several ions, such as calcium or magnesium ions, are reported to have regulatory effects on epidermal permeability barrier homeostasis. Recently, it has been suggested that strontium ion can play a substitutive role for calcium ion in various cellular reactions. Objective: This study was conducted to investigate the effects of strontium ion, either alone or in combination with calcium or magnesium ions, on epidermal permeability barrier homeostasis. Methods: Female hairless mice were used to study the effects of various ions on epidermal permeability barrier recovery. Calcium chloride solution, magnesium chloride solution or strontium chloride solution were topically applied to barrier-disrupted skin, either alone or simultaneously. Change of transepidermal water loss, which represents permeability barrier function, was measured by TEWameter and morphological change was also observed by light and electron microscopy. Results: Topical application of strontium chloride solution accelerated permeability barrier recovery rate, compared with vehicle-applied skin. Magnesium chloride solution also accelerated barrier recovery rate, as reported in previous studies. Interestingly, simultaneous application of strontium and calcium ions significantly accelerated barrier recovery rate, compared to application of strontium or calcium ion alone. Nile red staining confirmed the increased neutral lipid deposition in strontium ion applied skin. Electron microscopic observation also revealed an increased lamellar body secretion in strontium ion applied skin. Conclusion: Strontium ion can play a regulatory role in epidermal permeability barrier homeostasis due to, at least in part, its competitive action on calcium ion for the same ion channel. (Korean J Dermatol 2006;44(11):1309~1316)

아토피 질환과 알레르겐에 의한 피부 장벽 손상

김현정 ( Hyun Jung Kim ),정세규 ( Se Kyoo Jeong ),이승헌 ( Seung Hun Lee ) 한국피부장벽학회 2008 한국피부장벽학회지 Vol.10 No.2

Atopic dermatitis (AD) is a chronic inflammatory skin disease associated with a complex interplay between multiple factors, such as 1> strong genetic factors 2> the cutaneous hyper-reactivity to environmental trigger 3> neurologic and pharmacologic dysfunction 4> underlying defect in skin barrier function. Various contact allergens and aero allergens are focused as the important factors for the initiation of atopic diseases, aggravation of the diseases and therapeutic targets. In this review, the role of allergens in atopic disease can be explained in the view of various proteases, chitinase, abnormal skin barrier function and finally the vicious relationship with inflammation.

Linoleic Acid가 포함된 Multi-Lamellar Emulsion의 국소 도포가 실험적으로 유도된 면포 생성에 미치는 영향

이상은 ( Sang Eun Lee ),염종경 ( Jong Kyung Youm ),이승헌 ( Seung Hun Lee ),정세규 ( Se Kyoo Jeong ) 대한피부과학회 2007 대한피부과학회지 Vol.45 No.3

Background: Disturbed keratinization of the follicular infundibulum is the earliest change in comedo formation. The relative decrease in linoleic acid in the sebum could be responsible, in part, for this abnormal keratinization. Objective: This study was conducted to evaluate the effects of topically applied multi-lamellar emulsion containing linoleic acid (MLE/LA) on experimentally induced comedones. Methods: To induce comedo formation, 50% oleic acid (OL) in macrogol 400 was applied to the ventral surface of both ears of New Zealand white rabbits. Twenty ears of ten rabbits were randomly divided into four treatment groups (5 ears in each group). Four groups (OL only, OL and MLE/LA, OL and MLE, OL and control vehicle containing LA) were treated twice daily for 2 weeks. The relative increase in areas of the comedo was evaluated by digital image analysis. The morphologic changes around the epithelial lining of the comedo were observed by light microscopy and scanning electron microscopy. Results: After 2 weeks of application, only the OL and MLE/LA combined treated group showed significantly less (by 1.23-fold, p<0.05) increase in comedo size when compared to the OL treated group (by 1.86-fold). Upon light microscopy and scanning electron microscopy examination, the MLE/LA treated ears showed a lesser degree of epidermal hyperplasia and hyperkeratosis in the follicular infundibulum compared with the OL treated ears. Conclusion: Topical MLE/LA might have an inhibitory effect on the formation of OL induced comedones. (Korea J Dermatol 2007;45(3):249~254)

참느릅나무(Ulmus parvifolia) 잎 추출물 분석 및 안정성 평가

김두영 ( Doo-young Kim ),송수빈 ( Soobin Song ),김일주 ( Iljoo Kim ),정세규 ( Se-kyoo Jeong ),김성우 ( Sungwoo Kim ),김정희 ( Jung-hee Kim ),장현재 ( Hyun-jae Jang ),오세량 ( Sei-ryang Oh ),류형원 ( Hyung Won Ryu ) 한국응용생명화학회(구 한국농화학회) 2020 Journal of Applied Biological Chemistry (J. Appl. Vol.63 No.4

Ulmus parvifolia 잎의 약리학적 지표 성분을 제시하기 위해 간단하고 신뢰할 수 있는 HPLC 분석방법을 개발하였다. 지표 성분들은 NMR 및 UPLC-QTof-MS 분석에 의해 neochlorogenic acid (trans-5-O-caffeoylquinic acid, 5-CQA)과 chlorogenic acid (trans-3-O-caffeoylquinic acid, 3-CQA)로 구조 동정 되었다. HPLC를 이용하여 두개 화합물을 포함한 잎 추출물의 정성/정량 분석 방법을 평가하였다. 잎 30% 에탄올 추출물과 지표성분의 안정성 테스트는 6개월 동안 평가되었다. 그러나 안정성 조사 기간 동안 추출물과 지표 성분들 각각의 함량에 대한 유의한 변화는 관찰되지 않았다. A simple and reliable HPLC method was developed to determine pharmacologically standard marker compounds of Ulmus parvifolia leaves. Standard markers were characterized with neochlorogenic acid (trans-5-O-caffeoylquinic acid, 5-CQA) and chlorogenic acid (trans-3-O-caffeoylquinic acid, 3-CQA) using NMR and UPLC-QTof-MS analysis. A method for qualitative/ quantitative analysis of the leaves extracts were evaluated including two compounds by using HPLC. The stability test of 30% ethanolic extracts of the leaves sample and standard markers have been evaluated for six months. However, no significant changes in the content of the marker compounds of each extract was observed during the time of investigation.