Characterization of Algarose Product from Agar Using DMSO

전유진,이제희,Yasantha Athukorala 한국조류학회I 2005 ALGAE Vol.20 No.1

Agar was extracted from Gelidium amansii, which was harvested at the shores of Jeju Island in South Korea. As a unique solvent, the ability of dimethyl sulfoxide (DMSO) was used to separate agarose from agar by removing agaropectine and quality of the resultant agarose was characterized for chromatography purposes. Agar sample was agitated by motor-driven stirrer with DMSO in a water bath (at 70°C for 2 h) and centrifuged (3,000 rpm for 20 min). Resultant upper agarose layer was gelled, washed, dried and milled. The quality of agarose was evaluated by the analysis of proximate chemical composition, sulfate content, gelling strength and DNA migration. In this study, the separated agarose showed low sulfate amount (0.28%) and showed high gel strength (1190 g · cm–2). The resolution power and the ligase activities gave clear picture about the suitability of the present agarose for practical purposes.

$\alpha$-Chymotrypsin 을 이용한 Kyotorphin 유도체의 합성

전유진,김세권,Jeon, Yu Jin,Kim, Se Gwon 대한화학회 1994 대한화학회지 Vol.38 No.6

효소를 이용한 생리활성 펩티드의 합성 연구에 관한 기초자료를 얻기 위하여, Kyotorphin(진통작용을 가진 펩티드) 유도체가 $\alpha-chymotrysin$를 이용한 이상계(two phase system;유기상과 수용액상)조건하에서 Ac-Tyr-OH 와 $Arg-NH_2$로부터 합성되었다. Ac-Tyr-OH(10 mM)과 $Arg-NH_2$ (20 mM)와의 Kyotorphin 유도체 합성에 대한 유기용매의 효과에서 ethyl acetate계에서의 합성 수율이 다른 유기용매(dichloromethane, n-butanol, n-hexane, chloroform)에서 보다도 더 높았다. Kyotorphin 유도체의 합성에 미치는 최적조건을 보면, 효소 농도는 10 ${\mu}M$, 온도 및 pH는 각각 $35^{\circ}C$ 및 7.0이었으며, 유기상/수용액상의 비$(\alpha)$는 15였다. Kyotorphin 유도체 합성의 최적 반응조건하에서 수율은 70.2%였으며, 이때의 반응은 24시간 후에 평형에 도달하였다. In order to obtain the basic data for synthetic studies of bioactive peptide using enzyme, Kyotorphin(analgesic peptide) derivative was synthesized from Ac-Tyr-OH and $Arg-NH_2$ by $\alpha-chymotrysin$ in two phase system(organic phase and aqueous phase). In effect of organic solvent on Kyotorphin derivative synthesis from Ac-Tyr-OH(10 mM) and $Arg-NH_2$ (20 mM), the synthesis in ethyl acetate system of organic solvents was higher than those in other organic solvents (n-butanol, n-hexane, dichloromethane and chloroform). The optimal conditions for the synthesis are as follows: enzyme conc., 10 ${\mu}M;$ reaction pH, 7.0; reaction temp., $35^{\circ}C$ ; the ratio of organic phase volume/aqueous phase volume $(\alpha)$, 15. Under the optimal conditions, the yield was 70.2%, and the reaction achieved to equilibrium after 24 hrs.

< 수산가공분야 > : 가자미피 젤라틴을 이용한 유화제 개발

전유진,김세권 한국수산학회 1991 춘계학술발표회 Vol.- No.-

기능성 물질들의 검색을 위한 대체 동물모델로서 지브라피쉬

전유진,강성명 한국공업화학회 2012 한국공업화학회 연구논문 초록집 Vol.2012 No.-

키틴, 키토산 올리고당의 제조

전유진,김철호,김세권 한국키틴키토산학회 1998 한국키틴키토산학회지 Vol.3 No.2

Although chitin and chitosan have various bioactivities such as antimicrobial, antitumor by immunostimulating and elicitor acitivity, their effects in vivo were still ambiguous due to low uptake rates into the interior of the body. Therefore, converting chitin and chitosan to their oligosaccharides is necessary for more effect in vivo. There are two hydrolytic methods, acid hydrolysis and enzymatic hydrolysis, to prepare the oligosaccharides from chitin and chitosan. Acid hydrolysis of chitin and chitosan by inorganic acid led to lower oligosaccharides such monomer to pentamer in quantitative yield, but it gives only low yields of hexamer and heptamer. On the other hand, enzymatic hydrolysis of chitin and chitosan by chitinase and chitosanase have a lot of profits, comparing to acid hydrolysis, which are producing of more available oligosaccharides and more convenient preparing way. The present paper will review various useful methods for preparation of chitin and chitosan oligosaccharides among many literatures reported so far, especially focucing enzymatic hydrolysis and properties of the used enzyme, and valuable processing systems for the hydrolytic reaction

마찰력 저감 성능을 갖는 발수성 금속 표면 개발

전유진,Sumit Barthwal 대한기계학회 2021 대한기계학회 춘추학술대회 Vol.2021 No.05

제주산 해조류 감태의 생리활성 및 화장품 소재로서의 응용

전유진 한국공업화학회 2011 한국공업화학회 연구논문 초록집 Vol.2011 No.-

강아지 외 1편

전유진 고요아침 2022 한국동시조 Vol.- No.20

블루베리 (Vaccinium corymbosum L.) 유래 효소 추출물의 항산화성

전유진,김수현,Mahinda Senevirathne,하진환,김소미 한국생명과학회 2006 생명과학회지 Vol.16 No.1

Enzymatic extracts were prepared from the blueberry (Vaccinium corymbosum L.) collected in Jeju, Korea. Five carbohydrases namely AMG, Celluclast, Termamyl, Ultraflo and Viscozyme, and five proteases namely Alcalase, Flavourzyme, Kojizyme, Neutrase and Protamex were used to prepare the enzymatic extracts. Antioxidant properties of each extracts were studied using stable 1,1-diphenyl 2-picrylhydrazyl (DPPH), reactive oxygen species (ROS), nitric oxide (NO․) scavenging, metal chelating assays and lipid peroxidation inhibitory activity in hemoglobin-induced linoleic acid system. The phenolic content of all enzymatic extracts was in the range of 517.85–597.96 mg/100 g dried sample. DPPH and NO․ scavenging, and metal chelating assays exhibited prominent activities. Viscozyme showed the highest DPPH activity (0.046±0.002 mg/mL) while AMG showed the highest activity in NO․ scavenging (0.339±0.011 mg/mL). All the extracts exhibited strong metal chelating activities. Blueberry enzymatic extracts also showed relatively good activity in hydrogen peroxide scavenging. AMG showed the highest lipid peroxidation inhibitory activity (0.28±0.01 mg/mL) in hemoglobin-induced linoleic acid system. In this results, the blueberry, which has potential antioxidant components, may be a good candidate as a natural antioxidant source 제주지역에 서식하고 있는 블루베리 (Vaccinium corymbosum L.)로부터 수용성 추출물을 효과적으로 제조하기 위하여 5가지 종류의 탄수화물 분해효소 (AMG, Celluclast, Termamyl, Ultraflo, Viscozyme)와 또 다른 5종의 단백질 분해효소 (Alcalase, Flavourzyme, Kojizyme, Neutrase, Protamex)를 이용하여 효소적 추출을 시도하였다. 이들 효소를 이용하여 제조된 여러 가지 블루베리 효소 추출물에 대하여 1,1-diphenyl 2-picrylhydrazyl (DPPH), 활성산소종 (ROS), 일산화질소 (NO) 등의 소거활성, 금속 킬레이트결합능, 그리고 지질 과산화 저해능과 같은 항산화 효과를 검토하였다. 모든 효소 추출물의 페놀함량은 517.85~597.96 mg/100 g 건조시료이었으며, 항산화 효과에 대한 평가에서 특히 DPPH 및 NO 소거활성, 그리고 금속 킬레이트 결합능 등이 우수한 것으로 나타났다. Viscozyme 추출물은 DPPH 소거활성 (0.046±0.002 mg/mL)이, 그리고 AMG 추출물은 NO 소거활성 (0.339±0.011 mg/mL) 및 지질 과산화 억제활성 (0.28±0.01 mg/mL)이 각각 우수하였다. 과산화 수소 소거활성에 있어서는 블루베리 효소 추출물들이 천연 항산화제인 α-토코페롤보다 상대적으로 높았다. 이러한 결과로 볼 때 블루베리는 항산화 효과가 우수한 화합물들을 함유하고 있을 것으로 판단되며, 향후 천연 항산화 자원으로서 이용이 가능할 것으로 사료된다

Rosmarinic acid inhibits chemical hypoxia-induced cytotoxicity in primary cultured rat hepatocytes

전유진,송경식,한호재,박수현,장우철,이민영 대한약학회 2014 Archives of Pharmacal Research Vol.37 No.7

We examine the effect of rosmarinic acid (RA)in chemical hypoxia-induced injury in rat hepatocytes. Cellviability was significantly decreased by cobalt chloride(CoCl2), a well-known hypoxia mimetic agent in a timeanddose- dependent manner. RA pretreatment beforeexposure to CoCl2 significantly attenuated the CoCl2-induced decrease of cell viability. Additionally, pretreatmentwith RA potentiated the decrease of Bcl-2 expressionand attenuated the increase of Caspase-3 expression byCoCl2. CoCl2 treatment resulted in an increase of intracellularROS generation, which is inhibited by RA or N-acetylcysteine(NAC, a ROS scavenger), and p38MAPK phosphorylation,which is also blocked by RA or NAC. CoCl2-induced increase of Bax/Bcl-2 ratio and Caspase-3expression was attenuated by RA, NAC and SB203580 (p38MAPK inhibitor). CoCl2-induced decrease of cellviability was also attenuated by RA, NAC and SB203580pretreatment. Additionally, RA inhibited CoCl2-inducedCOX-2 expression and prostaglandin E2 (PGE2) secretion. Similar to the effect of RA, both NAC and NS-398 (COX-2inhibitor) blocked CoCl2-induced COX-2 expression andPGE2 secretion. NS-398 attenuated not only CoCl2-inducedincrease of Bax/Bcl-2 ratio and Caspase-3 expression, butdecrease of cell viability. Taken together, RA protects primarycultured rat hepatocytes against CoCl2-induced cellinjury through inhibition of ROS-activated p38MAPK andCOX-2/PGE2 pathway.