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고추역병균 ( 병원균 : Phytophthora capsici ) 의 생물학적 방제 - Ⅱ. 항균물질의 분리 정제 및 항균활성 -
장윤희,장상문,최정,이동훈 ( Yoon Hee Chang,Sang Moon Chang,Jyung Choi,Dong Hoon Lee ) 한국환경농학회 1996 한국환경농학회지 Vol.15 No.4
In the culture medium, the three antifungal fractions against P. capsici were separated by Sephadex G-25 column chromatography and Silica-gel chromatography. The substance A in white powder and the substance B in sticky oil were isolated by ethyl acetate: acetone mixture(7 : 3), and the substance C in yellow powder was isolated by chloroform: ethyl acetate mixture(95 : 5). The crude extract by ethyl acetate from the culture medium acidified to pH 2 was known to inhibit completely the growth of P. capsici at the level of 50㎎ ㎏^(-1). The substance A and B were known to be effective above the level of 5㎎ ㎏^(-1), and the substance C was effective above the level of 1㎎ ㎏^(-1). However, at the level of 20㎎ ㎏^(-1), the efficiency was in the order of A>C>B. It is apparent on a pot-experiment scale that the three substances effectively control Phytophthora blight of the red-pepper plant grown in the soil inoculated with P. capsici.
고추역병균 ( 병원균 : Phytophthora capsici ) 의 생물학적 방제 - Ⅰ. 고추 역병 길항균의 (拮抗菌) 선발 -
장윤희,장상문,이동훈,최정 ( Yoon Hee Chang,Sang Moon Chang,Dong Hoon Lee,Jyung Choi ) 한국환경농학회 1996 한국환경농학회지 Vol.15 No.3
This study was attempted to select an antagonist against Phytophthora blight of red-pepper caused by Phytophthora capsici. The three strains, A-35, A-67 and A-183 were isolated from the rhizosphere in soil where red-pepper had been cultivated continuously for a long time, and the strain A-83 was estimated to be the strongest antagonist against P. capsici. The A-183 strain was identified as a strain of Pseudomonas sp., showing the maximum antifungal activity, when cultured at 30℃ for 5 days in the potato extract medium(pH 6.5) containing 2.0% mannitol and 0.3% peptone.
무릎 신전 제한형 보조기 착용이 보행 시 하지 내 협응에 미치는 영향
장윤희(Yoonhee Chang),정보라(Bora Jeong),강성재(Sungjae Kang),류제청(Jeicheong Ryu),김규석(Gyu Seok Kim),문무성(Museong Mun),고창용(Chang-Yong Ko) 한국재활복지공학회 2016 재활복지공학회논문지 Vol.10 No.3
본 연구의 목적은 무릎 신전 제한형 보조기 착용 후 보행시 하지 내 협응에 미치는 영향을 평가하는 하는 것이다. 본 연구에는 7명의 건강한 성인 남성(32.3±2.7 세, 175.2±3.8cm, 76.2±8.7kg)이 참여하였다. 이들은 무릎보조기를 착용하거나 착용하지 않고 10m의 평지를 보행하였다. 이때 시공간적 파라미터, 하지의 각도, 하지 내 연속상대위상(CRP)를 측정 및 계산하였다. 그 결과 시공간적 요인은 차이가 없었다 (all p > 0.05). 무릎보조기 착용 시 엉덩관절의 가동범위 및 각도 변화는 없었으나, 무릎관절의 가동범위는 감소하였으며 (65.5±3.7° vs. 60.5±3.5°, p < 0.05), 최대 굴곡 역시 감소하였다 (입각기: 31.9±4.6 vs. 25.6±5.5, 유각기: 76.7±3.1 vs. 68.9±3.4, all p < 0.05). 발목관절의 가동범위는 변화가 없었으나, 최대 배측굴곡 (22.4±2.6 vs. 19.2±2.1, p< 0.05)은 감소하였으며, 최대 족저굴곡이 증가하였다 (9.5±3.0 vs. 15.7±2.2, p<0.05). 대부분의 CRP는 차이가 없었으며, 유각기 엉덩관절과 무릎관절 사이의 CRP만 감소하였다 (93.0±7.8 vs, 84.7±4.9, p < 0.05). CRP의 표준편차는 대부분 증가하였다 (유각기 엉덩관절과 무릎관절사이: 25.1±6.7 vs. 32.4±1.9, 입각기 무릎관절과 발목관절사이: 46.0±12.9 vs. 80.1±31.1, 유각기 무릎관절과 발목관절 사이: 34.5±4.1 vs. 37.6± 3.1, all p < 0.05). 이와 같은 결과로부터 무릎 신전 제한형 보조기 착용은 관절의 각도, 하지 내 협응에는 영향을 미치지만, 보행특성에는 큰 영향을 미치는 않는다는 것을 확인하였다. The aim of this study was to evaluate Influence on intra-limb coordination in individuals wearing knee brace during walking. Seven healthy male adults (32.3±2.7 years old, 175.2±3.8cm, 76.2±8.7kg) participated. They wore knee brace or didn`t wear any knee brace and were asked to walk along a 10 m long walkway. Spatiotemporal parameters, angles of the lower limbs, and intra-limb continuous relative phase (CRP) were measured and calculated. No differences of spatiotemporal parameters were shown (all p > 0.05). There were no changes in the angle and its range of motion (ROM) in the hip for the subjects as wearing knee brace, while ROM (65.5±3.7° vs. 60.5±3.5°, p < 0.05) of the angle and maximum flexion angles (stance: 31.9±4.6 vs. 25.6±5.5, swing: 76.7±3.1 vs. 68.9±3.4, all p < 0.05) in the knee significantly decreased. No changes in ROM of angle in the ankle were shown, whereas maximum dorsiflexion decreased (22.4±2.6 vs. 19.2±2.1, p< 0.05) and maximum plantarflexion increased (9.5±3.0 vs. 15.7±2.2, p<0.05). There were no changes in most of CRP between joints. CRP between the hip and knee joints decreased (93.0±7.8 vs, 84.7±4.9, p < 0.05). Most of CRP standard deviation increased (between the hip and ankle joint during swing: 25.1±6.7 vs. 32.4±1.9, between the knee and ankle joint during stance: 46.0±12.9 vs. 80.1±31.1, between the knee and ankle joint during swing: 34.5±4.1 vs. 37.6±3.1, all p < 0.05). These results indicated that wearing knee brace affected joint angle and intra-limb coordination, but less affected gait features.
장윤희(Yoon Hee Chang),고창용(Chang Yong Ko),정보라(Bo Ra Jeong),강정선(Jung Sun Kang),김규석(Gyoo Suk Kim),류제청(Jei Cheong Ryu) Korean Society for Precision Engineering 2018 한국정밀공학회지 Vol.35 No.1
Gait analysis is the best objective measurement tool for monitoring rehabilitation. However, it has limitations to evaluate gait recovery. Previous studies have evaluated the effect of gait training using continuous relative phase. The objective of this study was to determine the effect of gait recovery by rehabilitation gait training on lower limb coordination. We analyzed spatio-temporal parameters and CRP values of hip and knee joints based on gait analysis data obtained by 3D motion analysis system at 15 days intervals in 24 uni-lateral transfemoral amputees participated in IRP. Our results revealed that walking velocity of uni-lateral transfemoral amputees who participated in the program during a mean of 107.1 days was 49.2% faster than that at initial stage. The walking velocity showed a 46% increase at the end of 30 days after training. In gait coordination, values of CRP-RMS and CRP-SD were increased and maintained in-phase pattern. CRP showed symmetry in both limbs at the end of 90 days after training. Therefore, CRP is a significant factor in the gait recovery process. Effects of various rehabilitation training methods can be determined through CRP analysis.
베트남 한류문화 연구의 전개와 현황: 한국과 현지 학자의 한류연구주제와 접근법 비교를 중심으로
장윤희 ( Yoonhee Chang ) 세계음악학회 2018 음악과 문화 Vol.38 No.-
This paper examines academic approaches used and formed by Korean and Vietnamese researchers in studying Korean wave in Vietnam. This paper does not merely introduces existing outcomes done in the development the scholarship, but scrutinizes their topics, methodology, and purpose applied in the research. By focusing on the researchers' perspectives in looking at the Korean wave, this paper outlines research trend and paradigm shift that have been shaped from early 1990s until today. In the early stage, Korean scholars put emphasis on defining items of the most beloved and popular contents to tap market potentialities in Vietnam. On the other hand, the Vietnamese scholars looked at Korean language and its education as the initial content of the Korean culture related research. In the late 2000s, Vietnam saw the influence of Korean wave and showed their anxiety relevant to socio-cultural problems, while the Korean scholars turned their eyes to consumers, sales strategy, and new contents development in Vietnam. Recent researches pay attention to cultural confluence and Vietnamese pop culture along with the idea of discourse on "Vietnamese globalization" and self awareness of local people. This trend is also contemporary, cooperative, mutual, and human-oriented, and being attended with in depth interviews in the field. Policies, industries, education, and identity are all combined and intermingled in the past, present, and the future of Vietnam Korean wave scholarship. Thus the new scholars in different disciplines and perspectives have to be raised for the future research development on Korean wave in Vietnam.
장윤희(Yunhee Chang),유지형(Jihyung Yoo) 한국자동차공학회 2020 한국자동차공학회 학술대회 및 전시회 Vol.2020 No.11
An exploratory study on non-intrusive thermometry of permanent magnets based on optical fiber pyrometry is presented. This temperature sensor is very small and flexible, allowing precise temperature measurements in a small and compact devices. Furthermore, it is unaffected by electric and magnetic field interferences making it ideal for monitoring permanent magnet temperatures in electric motors. Near-infrared grey body radiation spectrum from a neodymium magnet from was recorded and processed. The measured temperature range was from 30℃ to 80℃. The measurement technique was also tested under various repetition rate from 250㎐ to 250㎑. The results showed good temperature sensitivity and low temperature uncertainty of around ±1℃ at the fastest tested repetition rate.
오이로부터 분리된 cucurbitacin B의 미백 효능 연구
장윤희 ( Yun-hee Chang ),추정하 ( Jung-ha Choo ),이소영 ( So-young Lee ),김태윤 ( Tae-yoon Kim ),진무현 ( Mu-hyun Jin ),장민열 ( Min-youl Chang ),이상화 ( Sang-hwa Lee ),이천구 ( Cheon-koo Lee ),박선규 ( Sun-gyoo Park ) 대한화장품학회 2014 대한화장품학회지 Vol.40 No.4
효능이 우수한 신규 미백 소재 개발을 위하여, 민간 및 전통 미백 처방에 사용되어 온 오이(Cucumis sativus L.)에서 활성 물질 분획 추적 연구를 통하여 cucurbitacin B를 분리 정제하고, cucurbitacin B의 멜라닌 합성에 미치는 효과를 B16F1 멜라노마 세포를 이용해 확인하였다. Cucurbitacin B는 세포 독성을 보이지 않는 농도에서 실험한 결과, 멜라닌 생합성을 농도 의존적으로 감소시켰다. Cucurbitacin B는 mushroom tyrosinase의 활성은 직접적으로 저해하지 않았지만, 세포에 처리했을 때 세포 내의 tyrosinase의 활성을 감소시킴을 확인하였다. 또한, cucurbitacin B의 이러한 멜라닌 합성 저해의 기전 연구를 위하여, 멜라닌 합성에 중요한 단백질인 tyrosinase와 microphthalmia-associated transcription factor (MITF)의 단백질 발현을 조사한 결과, cucurbitacin B가 tyrosinase와 MITF의 단백질의 발현을 농도 의존적으로 감소시키는 결과를 확인하였다. 또한, cucurbitacin B는 자외선에 의한 피부암 발생 억제인자(tumor repressor) 및 Wnt/β-catenin 신호전달 과정에 대한 억제 기능이 밝혀진 WW domain-containing oxidoreductase (WWOX)의 단백질 발현을 증가시킴을 추가적으로 확인하였다. 따라서, 이상의 연구 결과를 통해, 오이에서 분리 정제된 cucurbitacin B는 멜라닌 세포(melanocytes)에서 멜라닌 합성을 저해하는 효능이 있음을 확인하였으며, 향후 피부 미백 소재로 활용될 수 있을 것으로 판단된다. To develop an effective skin whitening agent for cosmetics, we isolated cucurbitacin B from Cucumis sativus L. which has been used as traditional skin lighting regimen by the bioactivity-guided fractionation, and investigated the inhibitory effects of cucurbitacin B on melanogenesis. At a non-cytotoxic concentration, cucurbitacin B reduced melanin contents of B16F1 melanoma cells in a dose-dependent manner. Cucurbitacin B did not directly inhibit mushroom tyrosinase activity, but it inhibited intracellular tyrosinase activity in a dose-dependent manner. Its inhibitory mechanism on melanin biosynthesis was further assessed, and we found that cucurbitacin B significantly decreased the protein level of tyrosinase, a major melanogenic enzymes and MITF, a master transcriptional factor of melanogenesis. In addition, cucurbitacin B increased the expression of WW domain-containing oxidoreductase (WWOX) which is known to function as tumor repressor and inhibits Wnt/β-catenin pathway. Collectively, these results suggest that cucuritacin B from C. sativus could be used as an active ingredient for skin whitening.