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      • Severe Escherichia coli Septicaemia and polyserositis in hens at the start of lay

        권용국,강민수,오재영,안병기,송은아,임춘태,권준헌 한국가금학회 2010 한국가금학회 정기총회 및 학술발표회 Vol.27 No.-

        The present study reports colibacillosis of layer chickens in a commercial egg-producing farm in middle part of Korean peninsular. Two flocks of chicken at 18-20 weeks of age were affected during the initiation of egg lay. They were characterized by no clinical signs, but sudden mortality, from 5%~7%, with severe lesions of speticaemia and fibrinous polyserositis. A Gram-negative bacterium was consistently isolated in pure culture from tissues. Isolates were typed biochemically as E. coli. The serotyping tests typed isolates as somatic group O1(7/10), O46(1/10), O78(1/10), O84(1/10). Interestingly, All the isolates were sensitive to enrofloxacin, ciprofloxacin, neomycin, cefepime, cefoxitin, ceftiofur. The isolates harbored the putative virulence genes, fimC, iucD and Iss. The results suggested that certain E. coli virulence genes, host factors and stress, such as initiation of egg lay may be associated with occurrence of colibacillosis.

      • 닭병원성 대장균과 야생조류유래 대장균 분리주에서 항생제 감수성 검사와 병원성 관련 유전자의 분포

        송은아,오재영,안병기,서이경,윤재영,강민수,임춘태,권준헌,권용국 한국가금학회 2010 한국가금학회 정기총회 및 학술발표회 Vol.27 No.-

        We compared the occurrence of antimicrobial resistance and virulenceassociated genes between 98 avian pathogenic Escherichia coli (APEC) and 790 E. coli strains isolated from normal wild birds. Of 15 antibiotics tested, 7 antibiotics such as nalidixic acid, tetracycline, ampicillin, sulfisoxazole, streptomycin, cephalothin, and enrofloxacin showed more than 30% resistance rate in APEC strains, whereas the resistance to E. coli (wild birds) strains was very low (Figure2). The 98 APEC strains and 120 of 790 E. coli strains (from wild birds) were detected the frequency of virulence-associated genes by multiplex PCR. The detection rates of virulence-associated genes tested in APEC strains were significantly higher than those of wild birds. The APEC strains comprised more than 40% against all 8 virulenceassociated genes. Although detection rates of virulence genes was low in E. coli strains of wild birds, they harbored almost every virulence-associated genes except for the cva A/B gene (Figure3). The distribution of virulence genes detected possesses twentyfive combination patterns in APEC strains, whereas E. coli strains of wild birds were divided into 14 gene combination patterns. According to antimicrobial disc tests and detection of virulence-associated genes, the APEC strains possesses multiple antibiotic resistance and virulence-associated genes which integrated into ColV plasmid with E. coli virulence. Even E. coli strains of wild birds also harbored various antimicrobial resistance and virulence markers.

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