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유기인계 살충제 Phosalone 원제 중의 불순물 동정 및 독성효과
임금춘,허장현,한대성 한국환경농학회 1995 한국환경농학회지 Vol.14 No.2
The purpose of this study was to investigate the identification and the toxicological effects of some impurities present in the technical grade phosalone (94.4%). In instrumental analyses of the technical phosalone, the five impurities such as phosalone oxon, 6-chloro-3-methylthio-2-oxobenzoxazole, 6-chloro-2-oxobenzoxazole, O,O,S-triethyl phosphorodithioate (OOSTEPDT) and dichlorophosalone were identified. The bimolecular inhibition rate constants (k_i) indicated that the technical phosalone inhibited both AChE and BuChE about ten times faster than the purified phosalone did. From in vivo studies the technical phosalone showed greater inhibition for mouse brain AChE, rat blood ChE's and mouse cytosolic non-specific esterases. It was presumed that some impurities present in the technical phosalone such as phosalone oxon cause such inhibition patterns of the technical phosalone observed in this study.
NaCl, 한발 및 온도 처리에 따른 유묘기 수도의 폴리펩티드 속성의 비교분석
임금춘,정영상,신정섭,Lim, Gum-Chun,Jung, Yeoung-Sang,Shin, Jeong-Sheop 한국응용생명화학회 1992 Applied Biological Chemistry (Appl Biol Chem) Vol.35 No.6
식물은 자극이 큰 환경적 stress에 반응 외견상의 변모 뿐 아니라 내적으로 생리적 생화학적인 변화가 있게 되며, 특히 체내의 단백질 합성계는 그러한 stress에 더욱 급격하게 반응한다. 여러가지 stress하에서 유묘기 벼의 단백질 수준이 어떻게 변화되는 가를 일차적으로 조사하기 위하여 NaCl, 한발 및 온도를 각각 달리 처리하여 이에 따른 폴리펩티드의 변이 양상을 전기영동에 의하여 비교하여 보았다. 환경 변화에 내성이 다소 강한 벼의 경우에도 stress 처리에 따라 다수의 폴리펩티드가 변화되었으며, 새로운 폴리펩티드의 생성도 관찰 되었다. 또한 기존의 1차 대사과정을 위한 단백질의 감소도 각각의 처리에 따라서 달리 표현되는 것이 확인되었다. Plants are altered not only in the outward appearance but also in their physiological and biochemical properties with reaction to the environmental stresses; particularly, the biosynthetic system of protein in situ rapidly responds to this. In order to investigate the change of properties of polypeptides in rice plants induced by several stresses, the seedlings were subjected to exposure to NaCl, drought, and low and high temperatures, respectively, and then some aspects of polypeptide variations were compared. Without exception, the rice plant, which is somewhat tolerant to environmental change, shows the alteration in several polypeptides. Moreover, newly synthesized polypeptides were observed in response to stresses. The existing proteins for the primary metabolic pathways were markedly decreased as each treatment progressed.
Phosalone 의 활성화과정을 통한 acetylcholinesterase 와 butyrylcholinesterase 에 대한 활성 저해
임금춘(Geum Choon Lim),허장현(Jang Hyun Hur),한대성(Dae Sung Han) 한국응용생명화학회 1995 Applied Biological Chemistry (Appl Biol Chem) Vol.38 No.2
The purpose of this study was to investigate a role of cytochrome P_(450), for the toxicity of the phosalone in in vitro and in vivo bioactivation systems. The bimolecular inhibition rate constants(k_i) of the phosalone to acetylcholinesterase(AChE) and butyrylcholinesterase(BuChE) were approximately 10²M^(-1)min^(-1), respectively, which meant a poor inhibitor. The potency of the phosalone as an inhibitor of AChE and BuChE was increased about 300 and 40 fold, respectively, when the inhibitor and the ChE were incubated with microsomes fortified with NADPH compared with microsome alone. Piperonyl butoxide(PB) addition to these coupled systems greatly reduced the inhibition of both target enzymes by blocking a bioactivation process. The I_(50), value of the Phosalone alone for rat brain AChE was 170 ㎎/㎏. When PB was pretreated, that value was altered to 42.5 ㎎/㎏. PB pretreatment synergized the inhibition of brain AChE with four times. Rat blood erythrocyte AChE and plasma BuChE were similarly inhibited in vivo by the phosalone and PB pretreatment didn`t affect significantly the pattern of the inhibition. The in vivo studies showed different results in the role of cytochrome P_(450) from those of the in vitro studies.