http://chineseinput.net/에서 pinyin(병음)방식으로 중국어를 변환할 수 있습니다.
변환된 중국어를 복사하여 사용하시면 됩니다.
Purification platform for Adeno associated virus using AAVR
이희형,이슬기림,김승현,조미라,장재형 한국공업화학회 2019 한국공업화학회 연구논문 초록집 Vol.2019 No.0
Since it is safe and effective as a gene carrier, AAV has recently been used in many gene therapy studies. However, because of the AAV production method, purification is essential to use AAV. However, AAV purification methods that are widely used have limitations. Some methods can’t be used to purify specific serotypes. And Other methods have cost limitations because they use expensive equipment or antibodies. To solve these limitations, AAVR, a protein that works with all serotypes of AAV, was used in this study. To expose AAVR on polycaprolactone(PCL), we used recombinant AAVR, manufactured PCL microsphere, and reformed the surface of PCL. As a result of study, AAVR-based AAV purification systems have shown similar or better results compared to the methods which is commonly used. There are still many things that need to be improved. However, I think this study will contribute to the production of AAV.
Polyelectrolyte for in vivo sustained gene/drug delivery
이희형,장재형 한국공업화학회 2019 한국공업화학회 연구논문 초록집 Vol.2019 No.1
Drug and gene delivery through intravascular injection spread throughout the body. So, Drug and gene delivery to target tissue locations is difficult. Therefore, direct injection into the target tissue is mostly for local delivery. However, without specific mediator, direct injection into the target tissue is difficult for sustained release because of the rate of diffusion of drugs and genes. Because of this, drug and gene delivery through mediators is essential. But an incision is necessary when the mediator is implanted through surgery. In order to minimize the incision site, injection should be possible. Therefore, this study was carried out by designing polyelectrolyte for the above-mentioned local & sustained drug and gene delivery. To produce the desired polyelectrolyte, we studied how to control the release rate of drugs and genes and reduce the immune response by controlling the combination of various materials applicable to the living body.
창유리 위에 졸겔 담금 방법으로 코팅된 인듐 주석 산화막의 에너지 절약 특성
정형진,이희형,이동헌,이전국 한국세라믹학회 1992 한국세라믹학회지 Vol.29 No.1
Indium tin oxide (ITO) layers are of considerable interest on account of the combination of properties they provide high electrical conductivity, high infrared reflection with high solar energy transmission, high transmission in the visible range. We are concerned about the variation of the spectral transmittances and sheet resistances as the thickness of SiO2-ZrO2 barrier layer and ITO layers and heat treating conditions are changed. Transmittances and reflectivities were studied by measuring UV-VIS-NIR-, FT-IR spectroscopy. ITO films are crack free, homogeneous and of polycrystalline cubic structure. The microstructure of good ITO films shows a narrow grain size distribution and mean value of 100 nm. The selectivity of absorbing properties is improved by increasing the thickness of ITO films. The increase of sheet resistance of ITO films are due to the increase in the reaction between films and glass substrate.
Polydopamine Coated Multi-Layered PCL Fiber as a Drug Delivery Carrier
오석민,김유진,이희형,한지나,( Lau Zheng Rong ),장재형 한국공업화학회 2019 한국공업화학회 연구논문 초록집 Vol.2019 No.0
The spontaneous formation of multi-layers by an electrospinning technique is an unconventional result considering that conventional electrospinning generates a non-discrete, continuous fibrous structure. Dopamine was used as a salt during the fabrication by electrospinning to fabricate multi-layered PCL fiber. The salts are conventionally removed from the fabricated fiber after fabrication. In this study, we have studied the uniform coating of fiber surface by polymerizing dopamine released from the multi-layered PCL fiber. the multi-layered PCL fiber uniformly coated by polydopamine was characterized by SEM, drug release profile in different pH conditions. Also, Adeno-associated virus (AAV) can be attached to the surface of the coating to enable sequential delivery of surface AAV and fiber internal drug. This multi-layered PCL fiber uniformly coated by polydopamine will contribute significant advancements into drug release control and gene therapy.
Surface tuning for controlled adeno-associated virus (AAV) delivery systems
김승현,이슬기림,이희형,조미라,장재형 한국공업화학회 2019 한국공업화학회 연구논문 초록집 Vol.2019 No.0
Multi-functional biomaterials based gene therapy could be utilized to enhance therapeutic effects (i.e. targeted delivery). Recently, adenoassociated viral vector (AAV) conducted biomaterials have been accelerating of researches, due to the final approval of commercialization by the food and drug administration (FDA, USA) and European medicines agency (EMA, EU). Herein, we developed the surface modification technique for interactions with any AAV serotypes. The recombinant AAV capture protein were engineered insertion of cysteine for maleimide-thiol “Click” chemical conjugation and produced for AAV conducting biomaterials. The tuning interfaces for AAV were showed that the significantly enhanced AAV capture efficiency and in vitro/in vivo controlled AAV transgene expression with any biomaterial structures (e.g., microspheres, nanofiber). This AAV specific functional biomaterial showed high potential for using advanced gene therapies (e.g. localized gene therapy).