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이용선,이근모,임새미 한국스포츠사회학회 2013 한국스포츠사회학회지 Vol.26 No.4
본 연구의 목적은 체육고등학교에 형성된 승리지상주의 문화를 분석하는데 있다. 이를 위해 전국 15개 체육고등학교 중 한 곳을 연구대상으로 선정하였고, 전략적 제보자 표집법을 통해 연구 참여자 15명을 선정하였다. 자료수집을 위해 비참여관찰, 심층면담, 관련자료수집을 사용하였고 수집된 자료는 텍스트 분석을 통해 결과를 도출하였다. 그 결과는 크게 세 가지로 나타났다. 첫째, 체육고등학교는 플래카드에 ‘신화성 문구’, ‘신체적·정신적 극기를 요구하는 문구’, ‘시합에서 갖춰야 할 신체적·정신적 태도에 대한 문구’를 넣고, 눈에 잘 보이는 장소에 부착하여 학생선수들의 심리를 자극하는 프로파간다 전략을 사용한다. 둘째, 체육고등학교 학생선수들이 전국대회에 출전하기 위해서는 1단계인 불편한 기초체력테스트(신체강화), 2단계인 승리를 위한 조건과 자극(정신강화), 3단계인 애국가와 교가 제창(소속감 확립)의 과정을 거쳐야 한다. 셋째, 체육고등학교는 모든 성적과 기록을 잘 보이는 장소에 공개함으로써 각 운동부 간의 경쟁을 부추긴다. The purpose of this study was to analyze the culture which is formed by winner-oriented in the physical education high school. To attain the goal, the paper selected one of the 15 high schools of physical education in Korea and chose 15 participants there through strategic informant sampling. To collect data, non-participant observation, in-depth interview, and related data collection were employed. The collected data drew results through text analysis. The forms of winner-oriented were seen largely from three aspects: First, The physical education high school posted placards marked with the ‘mythical phrases’, ‘phrases demanding physical and mental self-denial’, or ‘phrases about physical and mental attitudes for competitions’ to somewhere noticeable in order to stimulate student players’ psychology. Second, To enter national competitions, they had to pass three steps: The 1st step was the basal physical fitness test (physical enrichment), the 2nd step was English test and stimulation encouraging the win (mental enrichment), and the 3rd step was singing the national anthem and school song (enhancing a sense of belonging). Third, all teams in physical education high school incited competition by opening all results and records.
Expression of PACT and EIF2C2, Implicated in RNAiand MicroRNA Pathways, in Various Human Cell Lines
이용선,Yesu Jeon,Jong Hoon Park,Deog Su Hwang,Anindya Dutta 한국통합생물학회 2004 Animal cells and systems Vol.8 No.3
MicroRNA and siRNA (small interfering RNA), representative members of small RNA, exert their effects on target gene expression through association with protein complexes called miRNP (microRNA associated ribonucleoproteins) and RISC (RNA induced silencing complex), respectively. Although the protein complexes are yet to be fully characterized, human EIF2C2 protein has been identified as a component of both miRNP and RISC. In this report, we raised antiserum against EIF2C2 in order to begin understanding the protein complexes. An immunoblot result indicates that EIF2C2 protein is ubiquitously expressed in a variety of cell lines from human and mouse. EIF2C2 protein exists in both cellular compartments, as indicated by an immunoblot assay with a nuclear extract and a cytosolic fraction (S100 fraction) from HeLa S3 lysate. Depletion of EIF2C1 or EIF2C2 protein resulted in a decrease of microRNA, suggesting a possible role of these proteins in microRNA stability or biogenesis. We also prepared antiserum against dsRNA binding protein PACT, whose homologs in C. elegans and Drosophila are known to have a role in the RNAi (RNA interference) pathway. The expression of PACT protein was also observed in a wide range of cell lines.