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In vitro comet assay를 이용한 HaCaT 세포의피부 유전독성 예측
이선의 ( Seoneui Lee ),김아름누리 ( Areumnuri Kim ),신혜림 ( Hye Rim Shin ),전경미 ( Kyongmi Chon ),박경훈 ( Kyung-hun Park ),문병철 ( Byeong-chul Moon ),백민경 ( Min Kyoung Paik ) 한국환경농학회 2016 한국환경농학회 학술대회집 Vol.2016 No.-
Skin carcinogen such as inorganic arsenic has been shown to promote skin cancer by stimulating DNA damaging, tumorigenicity, carcinogenicity and cell apoptosis due to play a role in cell cycle control. Methyl methanesulfonate (MMS) is a genotoxic chemical and breaks DNA double-strand, and therefore it is used for a reference substances to induce DNA damage induction. HaCaT cells are immortal keratinocyte cell line from human skin.Genotoxicity in HaCaTcells provides some clues of genotoxic and carcinogeniceffects in skin tissue. The alkaline single cell gel electrophoresis (comet) assay is a sensitive and simple test for the detection of DNA damage at the individual cell level. Thus, in the present study, the comet assay was used to evaluate DNA damage and genotoxicity in HaCaT cells. DNA damage in HaCaT cells was elicited by treatment with MMS as positive control for 3 hrs.Theseresults ofDNA damage in HaCaT cells might reveal the possibility ofpredictingthe potential skin carcinogenicity.It is impossible of genotoxicity tests to detect perfectly with a single test, due to the fact that it is designed to detect on particular characteristic.Other genotoxicity tests such as in vitro micronucleus, Ames and lymphoma assay arerecommended, based on the standard test battery for genotoxicity.