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RISS 인기검색어
- 검색키워드
- 저자 : 이병웅 ( Soon Woo Hong (검색결과 2 건)
- 무료
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- 유료
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홍순우,하영칠,한홍의,이병웅,Hong, Soon-Woo,Hah, Yung-Chil,Han, Hong-Eui,Lee, Byung-Ung 생화학분자생물학회 1983 한국생화학회지 Vol.16 No.4
One strain of gram-negative bacteria which could not utilize furfural initially but could utilize 2-furoic acid as carbon source was isolated from the campus soil. This strain was identified as Pseudomonas fluorescens and the microorganism produced an extracellular metabolite that stimulates degradation of furfural, if $FeCl_3$ was provided to the furoic acid medium. The metabolite which was reactive with ninhydrin reagent (NPS; Ninhydrin Postive Substance) was purified by ion exchange chromatography and gel column chromatography. The empirical formula of NPS was determined as $C_2H_6NO_2$ by quantitative elemental analysis. The NPS also was assumed to be organic compound containing primary amide group by IR spectrophotometry and chemical analysis. 서울대학교내의 토양에서 furfural을 탄소원으로 이용하지는 못하나 이것의 대사물인 furoic acid를 탄소원으로 이용할 수 있는 세균을 분리하였으며 이것을 동정한 바 Pseudomonas fluorescens 임을 확인하였다. 이 P. fluorescens는 $FeCl_3$를 첨가한 furoic acid 배지에서 세포외에 furfural의 분해를 촉진하는 대사물 (ninhydrin반응 양성 물질 ; Ninhydrin Positive Substance=NPS)을 생성함을 알았다. 이 물질 즉 NPS를 ion exchange chromatography와 gel column chromatography에 의하여 정제하었다. 정량 원소 분석에 의하여서 이것의 실험식은 $C_2H_6NO_2$로 되어 있음을 밝혔고, 정성 분석과 IR spectrophotometry에 의해서 이것은 또한 일종의 primary amide기를 가진 물질임을 추정할 수 있었다.
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홍순우,하영칠,한홍의,이병웅 ( Soon Woo Hong,Yung Chil Hah,Hong Eui Han,Byung Ung Lee ) 생화학분자생물학회 1983 BMB Reports Vol.16 No.4
One strain of gram-negative bacteria which could not utilize furfural initially but could utilize 2-furoic acid as carbon source was isolated from the campus soil. This strain was identified as Pseudomonas fluorescens and the microorganism produced an extracellular metabolite that stimulates degradation of furfural, if FeCl₃ was provided to the furoic acid medium. The metabolite which was reactive with ninhydrin reagent (NPS; Ninhydrin Postive Substance) was purified by ion exchange chromatography and gel column chromatography. The empirical formula of NPS was determined as C₂H_6NO₂ by quantitative elemental analysis. The NPS also was assumed to be organic compound containing primary amide group by IR spectrophotometry and chemical analysis.
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