Development of chloroplast DNA markers for environmental monitoring of genetically modified Brassica napus and Brassica hybrids

이범규,Sohn Soo In 한국식물생명공학회 2023 Plant biotechnology reports Vol.17 No.4

The increasing cultivation and use of genetically modified (GM) crops has increased the importance of environmental monitoring for safety management. Brassica napus is the fourth most commonly cultivated GM crop globally, and it can hybridize with Brassica rapa or Brassica juncea by serving as a pollen donor or recipient for these species. When a hybrid of B. rapa or B. juncea with GM B. napus is detected in an outdoor environment, the safety management goals could determine whether B. napus is a pollen donor or recipient. In this study, two PCR (polymerase chain reaction) markers were developed to identify the paternal and maternal involvement of B. napus in hybrids with B. rapa or B. juncea based on the chloroplast genome data of B. napus. Further, Marker primer 07 differentiated B. napus from the other Brassica species, and Marker primer 20 differentiated B. napus from B. rapa and B. juncea. Applying the chloroplast and nuclear markers of the C and B genomes to the hybrids B. napus × B. rapa, B. rapa × B. napus, and B. juncea × B. napus using the PCR method showed that B. napus, B. rapa, B. juncea, and all hybrids could be distinguished. Thus, the effectiveness of these markers in identifying hybrids of GM B. napus in the natural environment has been demonstrated for environmental monitoring.

체리 과산화효소 고정 과산화수소 정량 효소전극의 전기화학적 고찰

이범규,윤길중,Lee, Beom-Gyu,Yoon, Kil-Joong 대한화학회 2015 대한화학회지 Vol.59 No.6

시내버스 노선망 위계구조의 효율성 분석 (대전시 사례분석을 중심으로)

이범규,장현봉 대한교통학회 2009 대한교통학회지 Vol.27 No.3

Four alternative plans for the bus network in Daejeon metropolitan city, which have different hierarchical structure, were proposed : Alternative 1 represents a bus network without hierarchical structure, and Alternative 2, 3, and 4 represent bus networks with primary, intermediate, and advanced hierarchical structures, respectively. Efficiency of the alternative plans were evaluated based on the evaluation index including travel time cost, waiting time cost, and transition penalty cost. The travel time cost was decreased as the level of hierarchical structure gets higher until it reaches the extremely high level. As the level of hierarchical structure get higher, the waiting time cost significantly decreased while the transition penalty cost increased. Collectively, a bus network with hierarchical structure was shown to be more efficient than without it in the light of total travel cost. For the bus network with hierarchical structure, total travel cost shows a concave curve, which implies that there exists an optimal level of hierarchical structure in a bus network. 대전시를 사례로 위계수준을 달리한 4개의 노선망 대안(대안 1은 위계가 없는 노선망, 대안 2, 대안 3, 대안 4는 각각 초급, 중급, 상급 수준의 위계를 가진 노선망)을 작성하고, 평가지표(차내통행시간비용, 대기시간비용, 환승페널티비용)를 산출하여 대안간 비교를 통해 효율성을 분석하였다. 차내통행시간비용은 위계가 높은 대안일수록 감소하다가 위계가 매우 높아지면 다시 증가하는 것으로 나타났다. 대기시간비용은 위계가 높은 대안일수록 큰 폭으로 감소하며, 환승페널티비용은 위계가 높은 대안일수록 증가하는 것으로 나타났다. 종합적으로 볼 때 총통행비용은 위계가 있는 노선망이 위계가 없는 노선망에 비하여 낮은 것으로 나타나 위계구조를 가진 노선망의 효율성이 높은 것으로 나타났다. 위계가 있는 노선망 중에서는 위계가 높을수록 총통행비용이 감소하다가 위계가 지나치게 높아지게 되면 오히려 증가하는 특성을 보임으로써 중급수준의 위계를 가진 노선망이 가장 효율성이 높은 것으로 분석되었다.

전북, 전남, 경남 지역 LM 유채 환경영향 모니터링 및 효율적 안전관리 기술개발

이범규,조사영,김동우,강종익,나수미,이훈복 한국환경생물학회 2020 한국환경생물학회 학술대회 Vol.2020 No.07

The complete chloroplast genome of Zoysia japonica Steud. isolated in Korea (Poaceae): investigation of potential molecular markers on Z. japonica chloroplast genomes

이범규,Park Jongsun 한국식물생명공학회 2021 Plant biotechnology reports Vol.15 No.5

Various cultivars of Zoysia japonica Steud. have been developed as genetically modified organisms for commercial purposes. The chloroplast genome was completed to investigate genetic diversity in Z. japonica isolated in Korea. The chloroplast genome of Z. japonica is 135,884 bp long (GC ratio is 38.4%) and has four subregions: 81,378 bp of large single copy (36.3%) and 12,582 bp of small single copy (32.7%) regions are separated by 20,962 bp of inverted repeat (44.1%) regions includ- ing 130 genes (83 protein-coding genes (PCGs), eight rRNAs, 38 tRNAs, and one pseudogene). 68 SNPs and 24 INDEL regions were identified from the two Z. japonica chloroplast genomes, similar to those of Z. matrella. 12 SNPs located in PCGs can be good candidates for developing molecular markers of Z. japonica. Based on nucleotide diversity analysis, atpB/ rbcL, rpl16/rps3, and rpl32/trnL regions exhibited the high nucleotide diversity, which can be used for developing species- specific marker sequences to distinguish their species. Phylogenetic analysis presented that two Z. japonica and Z. sinica were clustered together, indicating that they have been used for developing cultivars of Z. japonica.

고무로 결합된 과산화수소 감응 탄소반죽 전극의 빠른 감응

이범규,윤길중 한국과학수사학회 2015 과학수사학회지 Vol.9 No.4

과산화수소 정량을 위하여 고추냉이의 과산화효소를 효소원으로, 클로로술폰화 고무를 결합재로 사용하여 효소전극을 만들고 전압전류법으로 전기화학적 특성을 살펴보았다. 계가 보여주는 Lineweaver-Burk 도시의 직선성은 H2O2의 분해가 전극표면에 고정된 HRP에 의하여 촉진되고 있었고, 얻어진 대칭인자(α) 및 교환전류밀도(i0)를 포함한 10개 이상의 전기화학 파라미터는 전극이 정량적으로 작동하고 있음을 확인하여 주었다. 특히 2초보다 짧은 전극신호의 포화시간은 지금까지 소개된 과산화수소 감응 탄소반죽전극 중에서 가장 빠른 감응시간이었다. 이 같은 실험적 결과는 법과학 기법처럼 많은 시료의 신속한 분석을 요구하는 화학분석 분야에 응용될 수 있는 이론적인 기초를 제공할 것이다.

양이온 교환수지에 대한 희토류 원소의 흡수에 미치는 Coion의 영향

이범규,김인숙,김강진,Beom-Gyu Lee,In-sook Kim,Kang-Jin Kim 대한화학회 1983 대한화학회지 Vol.27 No.1

희토류 양이온이 4M 이상의 염산과 과염소산 용액에서 양이온 교환지수에 예상보다도 큰 흡수를 나타내는 현상을 알아보기 위하여, $1{\sim}12M 염산 또는 염산-과염소산 혼합용액에서 Dowex 50W-X2 양이온 교환지수에 흡수되는 Ce(III), Tb(III) 및 $Cl^-$이온의 양을 흡광분광법으로 측정하였다. 염산-과염소산 혼합용액에서 Ce(III)와 Tb(III)의 %S(흡수백분율) 값은 염산용액의 값에 비하여 모든 산농도에서 증가하였으며, 혼산중 과염소산의 분율이 증가함에 따라 과염소산만의 %S에 접근하는 것을 관찰하였다. 또 $Cl^-$ 이온의 흡수를 염산의 농도에 따라 측정해 봄으로써 수지내에서는 희토류 양이온과 $Cl^-$이온간의 착물로 존재하는 양은 많아야 흡수된 전체 희토류 이온의 10%에 불과해서 수지내에서의 이러한 착물형성이 중요하지 않은 것을 알았다. 혼산중의 과염소산 분율이 증가함에 따라 수지내 착물형성의 경향이 더욱 감소하였으므로 %S의 증가현상은 Cl-이온보다는 $ClO_4^-$이온의 영향이 더 클 것으로 예상된다. To understand the abnormal absorption behavior of rare earths in cation exchange resins, the absorption for Ce(III), Tb(III) and $Cl^-$ ions in Dowex 50W-X2 have been investigated by spectrophotometry in the concentration range of $1{\sim}12$ M HCI and $HCl-HClO_4$ mixed solutions. The amount of $Cl^-$ ion absorbed shows that the ratio of amount of $Cl^-$ ions to that of rare earths does not exceed 10% in the concentration range of $6{\sim}8M$ HCl and decreased gradually to 3% at 2M HCl and 6% at 12M HCl. The ratio is further decreased with the fraction of $HClO_4$ in $HCl-HClO_4$ mixed solutions and the decrease is presumably due to the weak tendency to form a complex between rare earths and $Cl^-$ ions in a cation exchange resin. The effect of $ClO_4^-$ is expected to play a more important role than that of $Cl^-$ ions in the large absorption of rare earths.

Cortisone投與가 肝디스토마 感染宿主와 蟲體에 미치는 影響

李範珪 연세대학교 의과대학 1968 연세의대논문집 Vol.1 No.1

Biotech. Info.

이범규,한정은,이종희,김현오 한국생물공학회 1996 BT NEWS Vol.3 No.3

제초제저항성 유채로부터 교잡근연종으로의 화분을 통한 유전자이동 조사

이범규,손수인 한국잔디학회 2019 Weed & Turfgrass Science Vol.8 No.2

The development and use of genetically modified (GM) crops have continuously increased. In particular, canola is the fourth largest GM crop in the world after soy, maize and cotton. Canola (Brassica napus) can hybridize with B. rapa, B. juncea and B. oleraceae, due to its genetic affinity with them, and is known to be hybridizable with various relatives. The commercialization of GM canola requires the establishment of risk assessment techniques. Canola has a relatively long pollen dispersal and can hybridize with wild species; thus, it requires rigorous safety assessment techniques for gene flow. This study investigated gene flow from canola to other Brassicaceae crops in the field area. Herbicide resistant canola was used as the pollen donor and placed at the center, and Chinese cabbage, turnip and leaf mustard were placed in four directions from the canola as pollen recipients. To analyze hybridization, seeds were harvested from the pollen recipient plants, and herbicide resistant seeds were selected by using an imidazolinone herbicide. Then, gene flow was verified through PCR analysis. Chinese cabbage showed a hybridization rate of 0.083% at the close distances of 0-6.1 m, and 0.033% at the distances of 9.15-15.25 m. Turnip showed a hybridization rate of 0.067% at 0-6.1 m from the pollen donor, but no turnip hybrid was found at the distances of 9.15-27.5 m. For leaf mustard, no hybrid was found in this experiment. At greater distances, one turnip hybrid was found at 30.5m and one Chinese cabbage hybrid at 64.05 m. It is expected that the results of this study would be used as basic data for establishing safety management practices for GM canola.