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무인원격 무선 네트워크 환경에서의 저전력 운용을 고려한 LP-MAC 기법
윤종택,류정규,김용이,Youn, Jong-Taek,Ryu, Jeong-Kyu,Kim, Yongi 한국정보통신학회 2014 한국정보통신학회논문지 Vol.18 No.8
원격 무선 네트워크 환경에서의 무인 센서 신호처리기의 임무 수행을 위해서는 한정된 전력 자원으로 인해 무인 원격 센서 무선운용 상황에 적합한 신뢰성 있는 저전력 매체 접속 제어 기법이 요구된다. 저전력 무선 네트워크에서 효과적인 신호 전송을 위해서는 CSMA/CA, X-MAC을 일반적으로 고려한다. 본 논문에서는 고정 노드로 구성되는 무선 네트워크에서 노드 제어를 위한 이동 노드의 망 참여/탈퇴가 유동적인 망 형태에서의 신속한 데이터 전달 및 소모 전력 최소화 달성이 가능한 저전력 성능이 향상된 LP-MAC 동작 기법을 제안한다. 고정노드는 망 자율구성을 수행하여 망에 수시로 참여/탈퇴하는 이동 노드로의 빠른 정보 전달을 위해 비동기 방식으로 동작한다. 이동 노드가 망에서 탈퇴할 경우, 망 전체 운용모드가 소모 전력의 최소화를 위한 동기모드로 천이됨으로써 최소 전력 운용이 가능한 매체접속 제어 기법이다. Because of the limited power resource, we need a reliable low-power media access control technique suitable for unmaned remote sensor operation condition for the unmanned sensor processor to perform the task in the remote wireless network situation. Therefore CSMA/CA and X-MAC is generally considered to effectively transmit the signal in the low-power wireless network. In this paper, we propose the more efficient low-power LP-MAC Technique which consumes the minimum power and transmits the data faster in condition that the mobile nodes' joining to and leaving from the network which consists of the fixed nodes is fluid. The fixed nodes operate in an asynchronous mode to perform the network self-configuration and transmit data faster to the mobile node which is frequently join and leave the network. When the mobile node leaves the network, the network's operation mode will be synchronous mode to achieve the minimum power consumption, thus the minimum power operation becomes possible.
Epidermal Growth Factor(EGF)와 Insulin-like Growth Factor-1(IGF-1)이 소 난포란의 체외성숙에 미치는 영향
윤종택,정영호,한기영,최선호 韓國受精卵移植學會 1998 한국동물생명공학회지 Vol.13 No.3
본 연구는 성장인자 EGF, IGF-1, EGF+IGF-1 이 소 난포란의 체외성숙에 미치는 영향을 규명하기 위하여 실시하였다. 소 난포란의 체외성숙 시 EGF를 농도별 (10, 50 및 100ng/m1)로 첨가하여 실험한 결과 통계학적 유의성은 인정되지 않았으나 24시간 배양 후 성숙율은 대조군에 비하여 높은 경향을 나타내었다. 소난포란의 체외성숙 시 EGF와 IGF-1을 병용 처리하여 실험한 결과, EGF 또는 IGF-1 단독처리 군에 비하여 병용처 The purpose of this study was to evaluate the effects of growth factors such as epidermal growth factor (EGF) and insulin-like growth factor-1 (IGF-1) on maturation of bovine follicular oocytes in vitro. Oocytes were recovered from the ovaries of slaughtered Hanwoos. The oocytes were matured in TCM 199 at 39, 5% in air. Growth factors were added to maturation medium as follows: control (no serum), EGF (10ng/m1, 50ng/ml or 100ng/m1), IGF-1 (100ng/m1) and EGF (50ng/ml) + IGF-1 (100ng/m1). The oocytes were placed onto a slide and stained with aceto-orcein dye. Nuclear maturation was evaluated and classified as germinal vesicle breakdown (GVBD), metaphase-I (MI) or metaphase-ll(Mll). Maturation rates were 37.9% (control), 45.8% (EGF, 10ng/m1), 55.8% (EGF, 50ng/ml), 44.4% (EGF, 100ng/m1), 46.7% (IGF-1, 100ng/m1) and 67.0% (IGF-1+EGF). The highest group developed to Mll stage was IGF-1+EGF treatment group (p<0.05). Therefore, nuclear maturation of bovine oocytes were affected by both of growth factors, and it seems to have a mutual activity between them.
소 난자의 체외수정 및 이후 발육에 영향을 미치는 요인들
윤종택,노상호,정연길,이호준,한기영 韓國受精卵移植學會 1999 한국동물생명공학회지 Vol.14 No.1
The objectives of the study were to establish sperm separation method and duration of insemination for bovine IVF. Oocytes from slaughterhouse ovaries were matured and fertilized using general protocol. After 18 or 42 h of insemination, six to ten embryos were placed into a 30 drop of each medium, and the embryos were examined 7~10d post in semination without medium renewal. First, we compared Percoll gradient will swim-up technique for sperm separation. There was no difference in cleavage rates between them, but the development rates over morula stage of oocytes fertilized with sperm separated by Percoll gradient was significantly higher than that sperm selected by swim-up technique (p<0.05). Second, we evaluated development of bovine embryos derived from the IVF procedure with different durations(18 vs 42 h) of fertilization. There was also no difference in cleavage rates, but the development to blastocyst stage of oocytes exposed in cleavage rates, but the development to blastocyst stage of oocytes exposed to sperm for 42 h was significantly higher than that exposed for 18 h (p<0.05). In conclusion, Percoll gradient can be used for sperm selecton, improving of embryonic development. Also, 42h of IVF may improve the development of bovine embryos.
소 체외수정란의 배양조건이 동결-융해 배반포의 생존에 미치는 영향
윤종택,이호준,노상호,정연길,박용습,최은주,이종완,김용엽,정혜영 韓國受精卵移植學會 1999 한국동물생명공학회지 Vol.14 No.3
This study was carried out to investigate the effect of co-culture system(bovine oviduct epithelial cells; BOEC) and defined culture system(modified TALP ; mTALP) on the development of IVM-IVF embryos, and survival of in vitro produced blastocysts after freezing and thawing. Occytes from the slaugheterhous ovaries were matured and fertilized using general protocol. The results obtained were as the following: 1. Survival rates of frozen-thawed blastocysts using 10% glycerol as cryoprotectant was higher in day 7 blastocysts than in Day 8 and 9 blastocysts from co-cultrue system, but survival rate of frozen-thawed blastocysts was higher in Day 10 blastocysts than in day 8 and 9 blastocysts from defined culture system. Regardless of their age, survival rate of frozen-thawed blastocysts was significantly higher (p<0.05) in co-culture system than in defined culture system. 2. The cell number of blastocysts was significanlty higher (p<0.05) in Day 7 blasotcysts than in Day 8 and 9 blastocysts from co-cultures, but the cell number of blsstocysts was significantly higher (p<0.05) in Day 10 blastocysts than in Day 8 and 9 blastocysts from defined culture system. Regardless of the culture system, blastocysts with higher cell number showed higher survival rates after freezing and thawing.
윤종택,이호준,노상호,정연길,손동수,김일화,류일선,김창근,정영채 韓國受精卵移植學會 1999 한국동물생명공학회지 Vol.14 No.3
This study was carried out to investigate the effect of co-culture system(bovine oviduct epithelial cells; BOEC) and defined culture system (modified TALP ; mTALP) on the conception of embryos transferred, and pregnancy and twin birth rates after transfer of fresh or frozen-thawed bovine blastocysts produced in vitro were also evaluated. Oocytes from the slaughterhouse ovaries were matured and fertilized using general protocol. The results obtained were as the following. The pregnancy rate after transfer was higher in co-culture group than in mTALP group, but was not signficantly different, and there is no difference between fresh embryo group and frozen-thawed embryo group in conception rate. The conception rate was not different whether 3∼4 blastocysts or 2 blatocysts transferred into a recipient, but the production rate of twin calves was significantly higher (p<0.05) when 3∼4 embryos transferred. The average birth weight of twin calves(24.38kg) was numerically, but not significantly lighter than that of single calves(26.68kg).