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      • 국산 가공치이즈와 외국산 가공치이즈의 지질에 관한 연구

        유제현,노상호,이강익 한국낙농학회 1987 韓國酪農學會誌 Vol.9 No.4

        1. 總脂質은 國産 S₁ 加工치이즈 27.76%, 國産 S₂加工치이즈 32.90%, KRAFT 加工치이즈 26.11%가 抽出되었으며, 그중 中性脂質, 糖脂質 및 燐脂質의 比率은 各各 99.09%:0.43%:0.48%, 99.32%:0.30%:0.38%, 98.92%:0.44%:0.64%였다. 2. 中性脂質 構成은 triglyceride가 國産 S₁加工치이즈 91.49%, 國産 S₂加工치이즈 87.83%, KRAFT 加工치이즈 82.95%로 가장 많았으며, 1,3-diglyceride, free fatty acid, 1,2-diglyceride, monoglyceride 等이 共通的으로 檢出되었으며, 특히 國産 S₁加工치이즈는 他種에 비해 遊離脂肪酸의 含量이 많았다. 3. 糖脂質 構成은 3種의 加工치이즈 모두 ceramide monohexoside, ceramide dihexoside, ceramide trihexoside가 主成分이었다. 3種의 加工치이즈가 ceramide dihexoside와 ceramide trihexoside 組成比에 있어서 그 pattern이 相異했고, 특히 國産 S₂加工치이즈에 있어서 ceramide trihexoside 含有量이 ceramide dihexoside 含有量에 비해 월등히 높았다. 4. 燐脂質 構成은 주로 phosphatidyl ethanolamine, phosphatidyl choline, sphingomyelin으로 構成되었고 이외에도 lysophosphatidyl choline이 微量 檢出되었다. 5. 中性脂質의 脂肪酸組成은 3種의 加工치이즈 모두 palmitic acid 가 가장 많았고, 다음으로 oleic acid, myristic acid, stearic acid 順으로 含有되어 있었다. 6. 糖脂質의 脂肪酸組成은 國産 S₁加工치이즈와 KRAFT 加工치이즈는 palmitic acid가 各各 31.75%, 30.64%로 가장 많았고, 國産 S₂加工치이즈에서는 oleic acid가 33.76%로 가장 많았으며, 共通的으로 炭素數 10 以下의 揮發性 低級脂肪酸은 徵量 含有되어 있었다. 7. 燐脂質의 脂肪酸組成은 3種의 加工치이즈에 있어서 共通的으로 oleic acid가 가장 많았고, 다음으로 palmitic acid, stearic acid 順으로 含有되어 있었으며, 國産 S₂加工치이즈의 경우 炭素數 20 以上인 高級脂肪酸이 0.67% 含有되어 他種에 비해 많았다. This study was undertaken to get the fundermental data for the manufacture of domestic process cheese. The composition of lipids in domestic and foreign process cheese (KRAFT) was examined by Thin-layer chromatography (TLC), densitometry and gas liquid chromatography. The results obtained were as follows: 1. The total lipid content and the ratio of neutral lipid, glycolipid and phospholipid in domestic process cheese S₁, S₂brand and foreign process cheese (KRAFT) were 27.76, 32.90, 26.11% and 99.09: 0.43: 0.48%, 99.32: 0.30: 0.38%, 98.92: 0.44: 0.64%, respectively. 2. In domestic process cheese S₁, S₂brand and foreign process cheese (KRAFT), the neutral lipid mainly consisted of triglyceride (91.49, 87.83, 82.95%), 1.3-diglyceride, 1.2-diglyceride, free-fatty acid and monoglyceride especially, free-fatty acid being larger in domestic process cheese S₁brand than in domestic process cheese S₂brand and foreign process cheese (KRAFT). 3. The glycolipid mainly consisted of ceramide monohexoside, ceramide dihexoside and ceramide trihexoside in common. The contents of these ceramide dihexoside and ceramide trihexoside showed much difference among them. Especially, the content of ceramide trihexoside was much higher than that of ceramide dihexoside in domestic process cheese of S₂brand. 4. The phospholipid mainly consisted of phosphatidyl ethanolamine, phosphatidyl choline and sphingomyelin. Besides, a very small amount of lysophosphatidyl choline was detected. 5. As for fatty acid composition of neutral lipid, palmitic acid had the highest amount of the three kinds of process cheese and followed by oleic, myristic and stearic acid orderly. 6. In the fatty acid composition of glycolipid, palmitic acid was distinctively the most abundant, being 31.75% in domestic process cheese of S₁brand, and 30.64% of foreign process cheese (KRAFT). The oleic acid was distinctively the most abundant (33.76%) in domestic process cheese of S₂brand. In common, very small amounts of the low molecular weight fatty acids were detected. 7. As for the fatty acid composition of phospholipid, oleic acid was in common the most abundant followed by palmitic acid and Stearic acid etc. in order. In the case of domestic process cheese S₂brand, the content of the long chain fatty acids with carbon number 20 or upward was 0.67% which was higher than any other process cheese.

      • KCI등재후보

        k - Casein , GMP , Sialic Acid 가 한우송아지 Rotavirus 와 제주도 Bovine Rotavirus ( JBR ) 의 MA - 104 세포감염에 미치는 영향

        유제현,김종헌,유대환,신원선,지병주,송진욱,김세민,박범석 한국축산식품학회 2002 한국축산식품학회지 Vol.22 No.3

        This study was conducted to investigate inhibitory effects of κ-casein, GMP and sialic acid addition on the infection of MA-104 cells by S97(Korean native cattle rotavirus) and JBR(Jeju island bovine rotavirus). MA-104 cells on incomplete M199 were infected with domestically separated S97 and JBR, activated by incubating at 37℃ for 6 days, and analyzed for the titer of rotavirus. κ-casein, GMP and sialic acid added MA-104 culture infected by activated S97 and JBR were incubated for 15 hours and stained by the AEC stainning method. The number of infected cells were counted on microscope. The titer of S97 and JBR was 2.5×10exp(7) and 2.0×10exp(6) PFU/ml, respectively. The inhibition level against cell infection by S97 was 97.4% for 2000μM of κ-casein and 97.44% for 2000μM of GMP. The inhibition level against cell infection by JBR was 99.52% for 2000μM of κ-casein and 99.78% for 2000μM of GMP. The inhibition level against cell infection by S97 and JBR was 3.85 and 3.63% for 2000μM of sialic acid, respectively. The high inhibitory effects (over 97%) of κ-casein and GMP against infection of MA-104 cells with S97 and JBR indicated great potentials for the use of κ-casein and GMP in the treatment of calf or infant caused by rotavirus.

      • KCI등재

        Clinical Outcomes of U-blade Gamma3 Nails Used to Treat Patients with Trochanteric Fractures: Retrospective Multicenter Study

        유제현,김상민,정호정,황지효 대한고관절학회 2019 Hip and Pelvis Vol.31 No.2

        Purpose: This study was performed to assess the radiologic and clinical results of U-blade Gamma3 nail use for the treatment of trochanteric fractures. Materials and Methods: Between September 2015 and May 2018, all patients aged 65 years and older who underwent surgery with U-blade Gamma3 nails were analyzed. A total of 129 patients were selected based on having at least six months of follow-up. Image evaluations included bone quality (T-score), fracture classification on plain radiograph (AO/OTA), computed tomography configuration, union period, position of lag screw, anatomical reduction, tip apex distance (TAD), sliding extent of lag screw, change of neck shaft angle, and complications leading to reoperations were analyzed. Functional outcome were assessed using the Koval grade (ambulatory ability) at the final follow-up. Results: The mean time to union was 19.7 (range, 6-36) weeks. The screw position was centric (93 cases; 72.1%) and anatomical reduction was achieved in 74 cases (57.4%). The mean TAD was 20.3 (range, 12.3-38.1)mm. The mean sliding length of the lag screws was 3.8 (range, 0.1-12.6) mm. The mean change of neck shaft angle was 3.4。(range, 0-12.8。). Reoperations were required in two cases (1.6%) due to the cutting out of the lag screw (n=1) and metal failure with U-blade bending (n=1). Finally, Koval grades for 49.8% of patients reached preoperative status. Conclusion: Overall, use of the U-blade Gamma3 nail led to favorable clinical results, suggesting that this system may be a good option for the treatment of trochanteric fractures.

      • KCI등재

        Osteoporotic Fracture: 2015 Position Statement of the Korean Society for Bone and Mineral Research

        유제현,문성환,하용찬,이동연,공현식,박시영,양규현 대한골대사학회 2015 대한골대사학회지 Vol.22 No.4

        Osteoporotic fractures are one of the most common causes of disability and a major contributor to medical care costs worldwide. Prior osteoporotic fracture at any site is one of the strongest risk factors for a new fracture, which occurs very soon after the first fracture. Bone mineral density (BMD) scan, a conventional diagnostic tool for osteoporosis, has clear limitations in diagnosing osteoporotic fractures and identifying the risk of subsequent fractures. Therefore, early and accurate diagnosis of osteoporotic fractures using the clinical definition which is applicable practically and independent of BMD, is essential for preventing subsequent fractures and reducing the socioeconomic burden of these fractures. Fractures caused by low-level trauma equivalent to a fall from a standing height or less at major (hip, spine, distal radius, and proximal humerus) or minor (pelvis, sacrum, ribs, distal femur and humerus, and ankle) sites in adults over age 50, should be first regarded as osteoporotic. In addition, if osteoporotic fractures are strongly suspected on history and physical examination even though there are no positive findings on conventional X-rays, more advanced imaging techniques such as computed tomography, bone scan, and magnetic resonance imaging are necessary as soon as possible.

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