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Roles of Nucleosides on Amino Acid Transport Systems of E. Coli
유승현,Yoo, Seung-Hyun 생화학분자생물학회 1985 한국생화학회지 Vol.18 No.1
E. coli를 배양하여 삼투에 의한 충격으로 막 내의 단백질을 추출시킨 후, 이들 E. coli의 아미노산 수송에 관여하는 Nucleoside의 영향을 관찰하였다. Pyrimidine Nucleoside인 Cytidine과 Uridine이 아미노산 수송을 크게 증가시켰는데 Purine Nucleoside들은 효과가 조금 뒤졌다. 증가된 아미노산 수송은 주로 세포내의 아미노산 pool에 도착되었고, 단백질이나 RNA합성을 저지하여도 이 수송은 영향을 받지않았다. 이런 자극 효과는 Nucleoside의 독특한 효과에 의한 것으로 밝혀졌다. Transport of a number of amino acids in osmotically shocked cells of Escherichia coli after growth in enriched media has been found to be enhanced by nucleosides, particularly cytidine and uridine which are more effective than purine ribonucleosides. Deoxyribonucleosides have activity approximately equivalent to that of the corresponding ribonucleosides. The stimulatory activity is involved primarily, in increasing the expandable pool of amino acid within the cell and is not significantly affected by inhibitors of protein or RNA synthesis. The stimulatory effect is specific to nucleosides because neither ribose, deoxyribose and free bases nor pseudouridine had any effect.
Evidence for Alkaline Phosphatase Involvement in tRNA-Stimulated Amino Acid Transport Systems
유승현,Yoo, Seung-Hyun 생화학분자생물학회 1985 한국생화학회지 Vol.18 No.1
세포막 단백질 중의 하나인 periplasmic 단백질이 적절한 tRNA의 존재하에 아미노산 수송기작에 관여함이 알려졌는데, 이 단백질이 alkaline phosphatase로 밝혀졌다. 이 단백질의 분자량은 gel여과 방법으로는 88,000, SDS전기 영동으로는 43,500으로 나타났는데 두 개의 동일한 단일체로 존재함을 나타내었다. 아미노산 분석과 효소 역할에 의거하여 alkaline phosphatase임이 알려졌고, 적절한 tRNA와 더불어 아미노산 수송을 크게 증가 시킴을 볼 수 있었다. A periplasmic protein which later turned out to be alkaline phosphatase has been found to participate in amino acid transport mechanisms of Escherichia coli in the presence of appropriate tRNA. The apparent molecular weight of the purified protein was estimated to be 88,000 by gel filtration and 43,500 by SDS-polyacrylamide gel electrophoresis, indicating this protein is a dimer composed of two identical monomers. The purified protein which showed tRNA-dependent amino acid transport activity in the shocked E. coli 9723 cells has been identified as alkaline phosphatase based on its amino acid composition and enzymatic activity in addition to the molecular size. Purified alkaline phosphatase or tRNA alone did not exert any significant effect on leucine, isoleucine or valine transport, whereas simultaneous addition of these two molecules increased the amino acid transports drastically.
이문구 소설의 특성 연구 -『관촌수필』, 『우리동네』 두 연작 소설을 중심으로-
유승현 ( Seung Hyun Yoo ) 우리어문학회 2006 우리어문연구 Vol.27 No.-
This study aims for clarifying the narrating method of Lee, Mun-ku`s novel, especially Gwanchonsupil and Wuridongne. Previous studies on Lee, Mun-ku`s novel is separated in two ways. One is social and cultural approaches which is based on the view of realism theory. The other is formal and aesthetical approaches which is based on the view of stylistics. However the aspect both of them mention in common is the writer`s peculiarity as a storyteller. It means that his novels are not written in the way normal modern novels are written but written in their own ways. And this makes the specialty of the way of Lee, Mun-ku`s storytelling. Both Gwanchonsupil and Wuridongne show the farm villages as backgrounds. But the farm village one of them show us is very different from another one. The farm village in Gwanchonsupil is the narrater`s hometown and the place which reminds the narrater past days everyone had lived happily. Therefore this novel express reflection and attachment for past days strongly. Wuridongne describes the lives of the farm village people as the present age. So it tells us the problem of the farm village very vividly by Lee, Mun-ku`s own way of storytelling. The reason Gwanchonsupil and Wuridongne shows us very different aspects, even though they have same background, is they are written by different ways of storytelling. Yet these different two novels show us the author`s special attitude in common. We can find the aspect as analyzing the way of storytelling of these two novels. Many studies on Lee, Mun-ku`s novel mention about lack of the author`s critical mind. This is because of the attitude revealed by the way of storytelling. This study pays attention to this point.
유승현(Seung-Hyun Yoo),김학균(Hak-Kyun Kim),김종범(Jong-Bum Kim),송준호(Joon-Ho Song),오대진(Dae-Jin Oh) (사)한국CDE학회 2009 한국CDE학회 논문집 Vol.14 No.2
This paper describes the design of the ossicular replacement prosthesis for the people who have problem of hearing because of middle ear disease. Also the simulation results of the designed product for verification by finite element analysis software (ABAQUS) are presented. New model is applied to middle ear FE analysis which was generated in previous study. The full replacements of ossicular prosthesis for ossicles (malleus, incus and stapes) are made of Hydroxyapatite and Titanium. Although other existing prosthesis models consider only sound amplification effect, current type has damper system which is operating on the audible frequencies. High frequency sound transmitted to inner ear can be reduced and the prosthesis and inner ear can be prevented from damage.
tRNA 의 자극을 받는 아미노산 수송 기작에 관여하는 Alkaline Phosphatase 의 증거
유승현 ( Seung Hyun Yoo ) 생화학분자생물학회 1985 BMB Reports Vol.18 No.1
A periplasmic protein which later turned out to be alkaline phosphatase has been found to participate in amino acid transport mechanisms of Escherichia coli in the presence of appropriate tRNA. The apparent molecular weight of the purified protein was estimated to be 88,000 by gel filtration and 43,500 by SDS-polyacrylamide gel electrophoresis, indicating this protein is a dieter composed of two identical monomers. The purified protein which showed tRNA-dependent amino acid transport activity in the shocked E. coli 9723 cells has been identified as alkaline phosphatase based on its amino acid composition and enzymatic activity in addition to the molecular size. Purified alkaline phosphatase or tRNA alone did not exert any significant effect on leucine, isoleucine or valine transport, whereas simultaneous addition of these two molecules increased the amino acid transports drastically.