http://chineseinput.net/에서 pinyin(병음)방식으로 중국어를 변환할 수 있습니다.
변환된 중국어를 복사하여 사용하시면 됩니다.
이준황,원병연,윤현철 한국바이오칩학회 2007 BioChip Journal Vol.1 No.1
The signal stability from electrochemical immunosensors based on the enzymatic back-filling strategy was investigated. The presence of an analyte, especially antibody, which is bound to the surfaceimmobilized capture molecule, could cause a significant signal loss during subsequent handling procedures and signaling due to its limited affinity /stability. Compared to the typical method of detection, the signal from the back-filling immunoassay is generated differently from the covalently immobilized (‘back-filled’) glucose oxidase (GOX) to the sensing surface, not from the bound analyte that is usually labeled with signaling molecules. Therefore, an enhancement of signal stability would be expected with the back-filling immunoassay. To evaluate the merits of the enzymatic back-filling method, various antibody dissociation conditions from immunosensor surfaces were compared in terms of signal stability and sensitivity. As a model immunosensing reaction, the 2, 4-dinitrophenyl (DNP) group was functionalized to the biorecognition surface on a thin-film gold electrode, and anti-DNP antibody was employed as the target analyte. Cyclic voltammetry was used for immunosensor signal registration and to trace the protein adsorption/dissociation process. The back-filling method was found to be suitable for the analysis of immune reactions without labeled-antibody molecules, and the signal stability was comparable to the affinity sensor employing biotin/avidin couple.
Washing-free Electrochemical Strategy to Detect Target DNA Utilizing Peroxidase Mimicking DNAzyme
이상모,Sujeong Shin,김효용,원병연,안준기,박기수,박현규 한국생물공학회 2020 Biotechnology and Bioprocess Engineering Vol.25 No.5
We herein describe a novel washing-free electrochemical strategy for target DNA detection by utilizing the peroxidase mimicking DNAzyme. The DNAzymeincorporated DNA probes, the key components of this strategy, are designed to be not able to form G-quadruplex structure in the initial state such that the peroxidase mimicking activity of DNAzyme is kept suppressed. The DNAzyme sequences, however, would be converted to the catalytically active G-quadruplex structure by the presence of target DNA, whose peroxidase mimicking activity then promote the precipitation reaction of 4-chloronaphthol (4- CN) on the electrode. Due to the precipitates produced on the electrode surface, the electrochemical reaction between the redox materials and electrode surface is inhibited, consequently leading to a significant increase of the impedance signal. Based on this novel electrochemical design principle, we successfully detected the target DNA from sexually transmitted disease (STD) pathogens such as Chlamydia trachomatis, Trichomonas vaginalis, and Herpes simplex virus type 2 in real patient samples, verifying its practical diagnostic capability in the clinical applications.
박수열(Soo-Yeol Park),원병연(Byung-Yeon Won),여금수(Keum-Soo Yeo),지창환(Chang-Hwan Ji),오영민(Young-Min Oh) 한국연안방재학회 2016 한국연안방재학회지 Vol.3 No.3
Light beacon structures are very essential to the maneuvering of ship especially in the night. The power is usually from the rechargeable battery connected to solar panel which generates electricity from the sunlight. There are two types of structures, one is floating and the other is fixed. In this study, we are dealing with the new power generation system motivated by the wave motion for the floating light beacon. The basic principle of new system is transforming wave motion into electricity by the solenoid that composed of circular tube coiled around and magnetic inside. We succeeded in generating electricity in the lab test using the motion actuator. On the basis of lab test experience it is applied to the real field, Samchuk port. The power unit is installed in the floating light buoy and generating electricity when the inclination of the buoy is beyond the certain limit. The amount of electricity depends on the wave height and wave period. The purpose of field test is to clarify the relationship between the wave condition and electricity.