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Nanocomposite Hydrogel Adhered to Concrete Material for Aquaculture of Marine Organism
원남일,복상현,박진숙,나양호 한국고분자학회 2018 Macromolecular Research Vol.26 No.8
Nanocomposite (NC) hydrogels based on N,N'-dimethyl acrylamide (DMAAm) using Laponite were prepared. The mechanical properties, adhesive properties to concrete material, and bio-fouling behavior of microalgae Nitzschia sp. were investigated. The elongation and fracture strength of the NC hydrogels were greatly increased and the elastic modulus was decreased in comparison with those of typical hydrogels prepared by using the organic cross-linking agent N,N'-methylenebis(acrylamide) (MBAA). In case of NC hydrogels, they had excellent adhesion properties to the concrete material. The adherence of microalgae on the NC hydrogel surface was also much higher. As the concentration of Laponite in the nanocomposite hydrogels increased, the elastic modulus and fracture strength increased, and the elongation at break, peeling force, and peeling energy were found to be at a maximum at certain concentrations.
연안재해가 연안환경에 미치는 영향과 학제간 환경연구의 중요성
원남일(Nam-Il Won),이상진(Sang-Jin Lee) 한국연안방재학회 2014 한국연안방재학회지 Vol.1 No.2
In this study, coastal ecosystem research on coastal disasters was shortly reviewed in terms of recent international publications and case studies of the 2011 East Japan Great Earthquake and Tsunami. Several keywords such as ecosystem, resilience, and tsunami were analyzed from recent 20-years publications in Web of Science. Most recent 10-year studies covered more than 90% of entire related studies. Tsunami and resilience were also importantly regarded in world leading journals such as Nature and Science, recently. The dramatic effects of the 2011 Japan earthquake and tsunami were found from various coastal systems of human dimension of villages, natural dimension of marine ecosystem, and research area such as coastal research facilities. The following international cooperative studies have been reporting both local and international marine effects of Fukushima-driven radionuclides as well as tsunami-driven debris. The on-going after-tsunami research projects are initiated to reveal the fluctuation and restoration process of coastal complex ecosystem and to provide sound environmental basis for possible socio-economic decision making such as resilient coastal system. This short review is expected to provide the most recent example to organize the future interdisciplinary research program for coastal disaster and environmental resilience in Korea.
한국 서해안에 서식하는 주황해변해면에서 분리된 해양세균 Microbulbifer sp.으로부터 생리활성물질 비올라세인의 규명
원남일 ( Nam-il Won ),이가은 ( Ga-eun Lee ),고기범 ( Keebeom Ko ),오동찬 ( Dong-chan Oh ),나양호 ( Yang Ho Na ),박진숙 ( Jin-sook Park ) 한국미생물생명공학회(구 한국산업미생물학회) 2017 한국미생물·생명공학회지 Vol.45 No.2
오늘날 해양생물로부터 얻어진 미생물유래의 이차대사물질은 구조적, 생물학적으로 새로운 화합물의 주요한 자원이다. 그 중에서 해면동물과 미생물 관계는 생리활성 물질을 탐색하는데 가장 흥미있는 자원 중 하나로서 주목받아 왔다. 본 연구에서는 서해안 조간대에서 채집된 주황해변해면(Hymeniacidon sinapium)으로부터 분리된 세균 균주(Microbulbifer sp., 127CP-12)를 검토하였다. 배양된 세균은 자주색 색소를 생산하였으며, 색소생산의 최적 배양조건을 조사하였다. 최대 색소생산을 위한 미생물 배양조건은 25℃, pH 6.0, 3% NaCl임을 알 수 있었다. 추출용매는 에탄올과 메탄올에 비해 아세톤이 더 적절한 것으로 나타났다. 추출된 색소의 주요성분은 HLPC, NMR, MS, 그리고 UV 스펙트럼의 구조 분석을 통해 유용한 생리활성물질인 비올라세인으로 밝혀졌다. 본 연구는 해양미생물이 관여한 대사물질로부터 생리활성물질을 조사하는 연구기법을 서술함과 동시에 오늘날 변화하는 해양환경에서 해면동물과 미생물 관계의 생태학적 의의를 제시하고 있다. Microbial secondary metabolites of marine organisms are regarded as major sources of structurally and biologically novel compounds with numerous potential uses. Sponge-microbe associations are among the most interesting sources for exploring bioactive compounds. In this study, the bacterial strain Microbulbifer sp. (127CP7-12) was isolated from the Asian marine sponge Hymeniacidon sinapium collected at an inter-tidal zone on the west coast of Korea. Cultured bacteria produced a violet pigment, and optimal culture conditions for violet pigment production were investigated. Maximum production of the violet pigment from the strain culture was observed under the conditions of 25℃, pH 6.0, and 3% NaCl. Acetone provided better extraction of the pigment from fermented broth compared with ethanol and methanol. The proposed structure of the major component in the extracted crude pigment was determined via high-performance liquid chromatography, nuclear magnetic resonance, mass spectrometry, and UV spectra analyses, which showed that the metabolite was the promising bioactive compound violacein. This study describes the examination of marine bioactive materials from microbe-engaged metabolites and the ecological implica-tions of the sponge-microbe association in a changing ocean.