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황용일,심상국,오시마 야스지,정동효 中央大學校 遺傳工學硏究所 1990 遺傳工學硏究論集 Vol.3 No.1
The cloned gene of glyceraldehyde-3-phosphate dehydrogenase(GAP) of Saccharomyces cerevisiae (Holland et al., 1983) has been characterized. Based on the communication, we have also cloned 2.1kb GAP DNA fragment and modified that fragment as a portable promoter. Two yeast exression vectors, one is a YCp type vector being maintained at low copy number(1 or 2) and the other is a YEp type vector at high copy number, have been constructed with the GAP promoter PH05' gene as an indicator gene. Our plasmids were introduced into Saccharomyces cerevisiae. HU-1, which has been improved. The Trp+ transformants expressed APase activity efficiently and showed the high level of PH05' transcripts.