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영지버섯 균사체를 이용한 고체 발효 쌍화탕의 급성독성에 관한 연구
엄영란(Youngran Um),박화용(Hwayong Park),이재훈(Jaehoon Lee),심기석(Kisuck Shim),마진열(Jinyeul Ma) 한국한의학연구원 2010 한국한의학연구원논문집 Vol.16 No.1
This study was carried out to investigate the acute toxicity and safety of Ssanghwa-tang extract fermented with Ganoderma lucidum. To evaluate the acute toxity and safety, 0(control group), 1250, 2500 and 5000 ㎎/㎏ of Ssanghwa-tang and fermented Ssanghwa-tang extracts were orally administered to 20 male and 20 female ICR mice. After single administration, we observed survival rates, general toxicity, changes of body weight, and autopsy. Compared with the control group, we could not find any toxic alteration in all treated groups (1250, 2500 and 5000 ㎎/㎏). LD?? of Ssanghwa-tang and fermented Ssanghwa-tang extracts might be over 5000 ㎎/㎏ and it is very safe to ICR mice.
엄영란(Youngran Um),심기석(Kishuk Shim),이재훈(Jaehoon Lee),박화용(Hwayong Park),마진열(Jinyeul Ma) 한국한의학연구원 2009 한국한의학연구원논문집 Vol.15 No.1
The aim of this study was to study the quantitative analysis of glycyrrhizic acid in Glycyrrhizae Radix extract fermented with Paecilomyces japonica, Ganoderma lucidum, honey or Nuruk. The amounts of dry on loss were measured and the quantitative analysis of glycyrrhizic acid was performed by high performance liquid chromatographic (HPLC). HPLC method was performed on C18 column (250 ㎜ × 4.6 ㎜, 5 ㎛, RS tech) using gradient solvent mixtures of water-acetonitrile with photodiode array detector (254 ㎚). The flow rate was 1.0 ㎖/min. Retention time of glycyrrhizic acid was about 23.96 min and linearity of calibration was R²=0.9998. Contents of glycyrrhizic acid in Glycyrrhizae Radix extract (control) was 5.048 ± 0.14; Contents of glycyrrhizic acid in Glycyrrhizae Radix extract fermented with Paecilomyces japonica (SDT) was 1.975 ± 0.07; Contents of glycyrrhizic acid in Glycyrrhizae Radix extract fermented with Ganoderma lucidum (SYT) was 2.676 ± 0.07; Contents of glycyrrhizic acid in Glycyrrhizae Radix extract fermented with honey (SST) was 5.191 ± 0.06; Contents of glycyrrhizic acid in Glycyrrhizae Radix extract fermented with Nuruk (SNT) was 5.305 ± 0.34, respectively. Contents of glycyrrhizic acid in SDT and SYT were decreased but that in SST and SNT was increased when compared to control.
엄영란(Youngran Um),이지혜(Jihye Lee),마진열(Jinyeul Ma) 한국한의학연구원 2010 한국한의학연구원논문집 Vol.16 No.1
The purpose of this study was investigation of quantitative analysis of marker substances in solid fermented Angelicae Gigantis Radix by High performance liquid chromatography(HPLC). HPLC was performed for determination of nodakenin and decursin in solid fermented Angelicae Gigantis Radix extract, the separation method was performed on C18 column (250 ㎜ × 4.6 ㎜, 5 ㎛, RS tech) using gradient solvent mixtures of water-acetonitrile with photodiode array detector (330 nm). The flow rate was 1.0 ㎖/min. Retention time of nodakenin and decursin was about 11.47, 46.79 min and linearity of calibration was showed good result(r2=0.9999, 0.9999), respectively. Content of nodakenin was 0.76 ± 0.02% in control, 0.31 ± 0.00% in Angelicae Gigantis Radix extract fermented with Paecilomyces japonica(SDT)(p<0.01), 0.51 ± 0.02% in Angelicae Gigantis Radix extract fermented with Ganoderma lucidum(SYT)(p<0.01), 0.82 ± 0.03% in Angelicae Gigantis Radix extract fermented with honey(SST)(p<0.05) and 0.88 ± 0.01% in Angelicae Gigantis Radix extract fermented with Nuruk(SNT)(p<0.01). Content of decursin was 4.50 ± 0.08% in control, 2.90 ± 0.05% in Angelicae Gigantis Radix extract fermented with Paecilomyces japonica(SDT)(p<0.01), 2.65 ± 0.08% in Angelicae Gigantis Radix extract fermented with Ganoderma lucidum(SYT)(p<0.01), 4.46 ± 0.11% in Angelicae Gigantis Radix extract fermented with honey(SST) and 4.73 ± 0.04% in Angelicae Gigantis Radix extract fermented with Nuruk(SNT)(p<0.05), respectively.
이지혜(Jihye Lee),엄영란(Youngran Um),박화용(Hwayong Park),이재훈(Jaehoon Lee),마진열(Jinyeul Ma) 한국한의학연구원 2009 한국한의학연구원논문집 Vol.15 No.2
The purpose of this study was investigation of quantitative analysis of marker substances in Paeonia lactiflora extracts by solid fermentation. High performance liquid chromatography (HPLC) for the determination of albiflorin and paeoniflorin in P. lactiflora extracts by solid fermentation, the separation method was performed on C18 column (250 ㎜ × 4.6 ㎜, 5 ㎛, RS tech) using gradient solvent mixtures of water-acetonitrile with photodiode array detector (230㎚). The flow rate was 1.0 ㎖/min. Retention time of albiflorin and paeoniflorin was about 28.88, 31.92 min and linearity of calibration was showed good result(r2 = 0.9998, 0.9996), respectively. Content of albiflorin was 0.090 ± 0.03% in P. lactiflora extract(control), 0.102 ± 0.00% in P. lactiflora extract fermented with Paecilomyces japonica, 0.056 ± 0.01% in P. lactiflora extract fermented with Ganoderma lucidum, 0.093 ± 0.00% in P. lactiflora extract fermented with honey and 0.046 ± 0.00% in P. lactiflora extract fermented with Nuruk. Content of paeoniflorin was 4.506 ± 0.13% in control, 2.599 ± 0.04% in P. lactiflora extract fermented with Paecilomyces japonica, 1.222 ± 0.03% in P. lactiflora extract fermented with Ganoderma lucidum, 2.750 ± 0.05% in P. lactiflora extract fermented with honey and 0.847 ± 0.00% in P. lactiflora extract fermented with Nuruk, respectively. Content of the marker substances did not increase in all fermentation experiment group.