역 모낭성 각화증의 임상적 고찰 및 Human Papillomavirus의 검색에 관한 연구

안성구,이인욱,최응호,이원수,이승헌,이동원 ( In Wook Lee,Eung Ho Choi,Sung Ku Ahn,Won Soo Lee,Seung Hun Lee,Dong Won Lee ) 대한피부과학회 1997 大韓皮膚科學會誌 Vol.35 No.6

Background : Inverted follicular keratosis has distinctive histopathological features and is infre quently encountered by dermatologists. To date, the pathogenesis of inverted follicular keratosis has not, been concluded. Many authors have suggest,ed several theories for the nature of invert.ed follicular keratosis including vearuca vulgaris with squamous eddies, irritated seborrheic keratosis and dist,inctive follicular tumors. Objective : It is the purpose of this study to observe and compare the clinical features of in verted follicular keratosis with previous studies and evaluate the possible relationship between in verted follicular keratosis and human papillomavirus to clarify the nature of this condition. Methods : We studied 9 patients with inverted follicular keratosis for clinical features including the duratiori of the disease, the location and size of the lesions, the clinical impressions at the first visit and we made a record of the patients age and sex. To evaluate the relationship be tween inverted follicular kerato is and the human papillomavirus, we performed immunohistochvm ical staining using polyclonal antibodies to the human papillomavirus common antigen. We also used the polymerase chain relation to detect hurnan papillomavirus DNA in inverted follicular keratosis from paraffin embedded tissue preparation. Results : 1. Seven of the 9 patients,vere male and the ages ranged fiom 36 to 77 years(mean 53 years). The lesions were situat d on the face(4/9),abdomen(1/9), back(1/9), chest(1/9), scapular area(1/9) and leg(1/9). The average size of the lesions was 1.5 cm. Verruca vulgaris was the most common clinical diagnosis(3/9), followed by seborrheic keratosis(2/9), soft fibroma(2/9), pigmented nevi(1/9), and granioloma pyogenicum(1/9). 2. Immunohistochemical stainiiigs using polyclonal antibodies to the human papillomavirus common antigen were negative for all 9 cases. 3. Polymerase chain reaction; using primers for human papillomavirus DNA were negative for all 9 cases. Conclusipn : In general, our linical findings are similar to those of others in previous studies. Our results of immunohistocheriiical staining and the polymerase chain reactions suggest, that in vert,ed follicular keratosis is ncl, related to the human papillomavirus. Further investigat.ions inf'o the nature of invert,ed follicular keratosis, should be centered on initated seborrheic keratosis and distinct follicular tumor s. (Korean J Dermatol 1997;35(6): 1074-1081)

최근 병원 내원 여드름 환자의 통계적 고찰

이승헌 ( Seung Hun Lee ),박태현 ( Tae Hyun Park ),강원형 ( Won Hyoung Kang ),황규광 ( Kyu Kwang Whang ),이창우 ( Chang Woo Lee ),성경제 ( Kyung Jeh Sung ),안성구 ( Sung Ku Ahn ),박장규 ( Jang Kyu Park ) 대한피부과학회 1996 대한피부과학회지 Vol.34 No.3

Background: Many patients with acne have little information about its cause, course, and treatment although it is one of the most common skin diseases seen at hospitals. Objective : We would like to find out what acne patients think about the etiology of acne, what factors affect its course their treatment methods before going to hospital, and the psychological impact of acne. Methods : We classified the clinical levels of acne patients who visited seven university hospitals for the first time. We distributed questionnaires to them and analysed the results statistically. Results : 1) Patients were classified,according to their level of clinical severity : mild type(45.0%), moderate type(46.2%), and seuere type(8.8%). 2) The mean age at onset was 15.7 in males and 16.7 in females. 3) 69.3% of patients experienced that psychological stress had aggravated acne. 4) 42.8% of patients experienced that certain foods had aggravated acne. 5) 66.8% of female patients experienced aggravation of acne after starting to wear make-up. 6) 25.1% of female patients complained of a flare-up 1-7 days premenstrually. Conclusion : Acne is affected by many different factors, but there seems to be an especially close relationship between acne and the psychological factors which influence social life. (Kor J Dermatol 1996;34(3): 386-393)

Benzalkonium chloride 자극 후 desoxymethasone 연고 , vaseline 및 hydrobase 도포에 따른 피부장벽의 회복

안성구 ( Sung Ku Ahn ),최응호 ( Eung Ho Choi ),강소군 ( Jiang Shaojun ),황상민 ( Sang Min Hwang ),이승헌 ( Seung Hun Lee ) 대한피부과학회 1998 대한피부과학회지 Vol.36 No.5

Backgroud: Topical irritants disrupt the cutaneous permeability barrier through the removal of stratum comeum lipids. This perturbation of barrier integrity stimulates a variety of homeostatic repair responses that ultimately result in the normalization of bamer function. Object To measure the effect of desoxymethasone ointment, vaseline and hydrobase on the barrier recovery of benzalkonium chloride (BKC) imtated skin Materials and methods . The left flank skin of 2-3 monthold hairless mice was treated with BKC and then desoxymethasone ointment, vaseline and hydrobase were applied. Transepidermal water loss (TEWL) was checked after 0, 3, 6, 9, 12, 15, 18, 21 and 24 hours. Electron microscopic examination was performed after 3 and 24 hours after desoxymethasone, vaseline and hydrobase had been applied. Results : The recovery of TEWL was most prominantly observed in the desoxymethasone ointment treated group followed by vaseline and hydrobase. Electron microscopic examination using ruthenium tetroxide fixation revealed that secretion and numbers of lamellar bodies and complete formatice of lipid bilayers were most prominent at desoxymethasone ointment and vaseline treated group. Conclusion : Desoxymethasone ointment, vaseline and hydrobase can be good agents in improving bamer recovery after exposure to irritant material. (Korean J Dermatol 1998;36(5): 820-826)

무모생쥐에서 아세톤 자극후 스테로이드의 국소도포 및 스테로이드 주사에 의한 피부장벽의 회복효과

안성구(Sung Ku Ahn),천승현(Seung Hyun Chun),최응호(Eung Ho Choi),이승헌(Seung Hun Lee) 대한피부과학회 2002 대한피부과학회지 Vol.40 No.10

N/A Background : Acetone disrupts the cutaneous permeability barrier thtough the removal of stratum corneum lipids. This pertubation of barrier integrtity stimulates a variety of homeostatic repair that ultimately results n the normalization of barrier function. Object : To measure the effect of steroid on the barrier recovery of acetone applied skin, Material and Methods : The flank skin of 8∼10week old hairless mice was treated with acetone and then topical and systemic steroids were applied. Transepidermal water loss(TEWL) was checked after 0, 3, 6, 12 and 24 hours. Electron and light microrcopic examination and ion capture cytochemistry were performed after 3, 6, 12 and 24 hours ter systemic and topical steroids had been applied. Results The results were as follows ; 1) During 3∼6 hours after experiment, the recovery rate of TEWL was most prominent in the group of acetone applied animal than other group. 2) After 12 hours after acetone applied, formation of new stratum corneum was found in the groups of acetone applied or acetone applied skin with topical steroid application. But loss of stratum corneum was observed in the groups of high or low dose steroid injection. 3) Ruthenium tetroxide staining of acetone alone or topical steroid treated specimens after 12 hours experiment revealed that the lipid bilayer was partly impaired and fragmented, Intercellular spaces were widening and the lipid bilayer disappeared or was damaged in the groups of high or low dose steroid injection. 4) Six hours after acetone application, pattern of calcium distribution had been partially reestabilished in the group of acetone alone or topical steroid treated animals. But calcium content was still sparse and decreased from the stratum granulosum to basale in the groups of high or low does steroid injection.

장시간 수화가 표피투과장벽에 미치는 영향

안성구 ( Sung Ku Ahn ),이은희 ( Eun Hee Lee ),홍승필 ( Seung Phil Hong ),최응호 ( Eung Ho Choi ),이승헌 ( Seung Hun Lee ) 대한피부과학회 2007 大韓皮膚科學會誌 Vol.45 No.1

Background: Water exposure is considered an important causative factor of irritant contact dermatitis. It is also known that water exposure can disrupt the stratum corneum (SC). However, there are only a few morphologic studies on the effect of water contact on the skin. Objective: The aim of our study was to investigate the effects of prolonged water exposure on the permeability barrier and the ultrastructure of the SC intercellular lipids. Methods: After prolonged water exposure of hairless mouse skin in vivo for 24, 36, 48, and 72 hrs respectively, the permeability barrier function was assessed by transepidermal water loss (TEWL) measurement, and the ultrastructure of SC by electron microscopy using osmium tetraoxide and ruthenium tetraoxide postfixation and calcium ion capture cytochemistry. Additionally, the lipid composition was evaluated using confocal microscopy with nile red stain and the integrity of the SC assessed using a lanthanum tracer. Results: After prolonged water exposure, water caused a significant increase in TEWL with disappearance of the calcium gradient, but this did not significantly influence the recovery rate of TEWL. The intercellular lipids were disrupted, and multiple lacunae containing abnormal delaminated materials within the intercellular spaces were observed. Lanthanum tracer penetrated into the intercellular space of the SC. There was a progressive decrease in nile red staining with neutral lipid content. With increasing exposure to water, these results were more evident. Conclusion: Our results provide a better understanding of the disruptive effect of prolonged water exposure on barrier lipids, the penetration-enhancing effect of water and the increased susceptibility to irritants, with regard to duration of water exposure. (Korean J Dermatol 2007;45(1):23~33)

피부섬유종의 임상양상과 유병기간에 따른 병리조직학적 및 면역조직학적 고찰

안성구(Sung Ku Ahn),이남호(Nam Ho Lee),강윤철(Yun Chul Kang),최응호(Eung Ho Choi),황상민(Sang Min Hwang),이승헌(Seung Hun Lee) 대한피부과학회 2000 大韓皮膚科學會誌 Vol.38 No.4

N/A Background:Dermatofibroma is a common benign fibrohistiocytic lesion that occurs most commonly on the legs of middle-aged women. Objective:The study was performed to evaluate the clinical, histopathological and immunohistochemical features of dermatofibromas according to the duration. Methods:Sixty seven cases of dermatofibromas were selected from 12 years of laboratory files for the period January 1986 to June 1998 from two medical hospitals. Results:1. The age range was from 10 to 69 and the average age at diagnosis was 36.0 (male;38.3, female;34.5). The ratio of male to female was 1.0:1.5 (27:40). 2. Fifty eight cases(86.5%) had solitary lesions, and nine cases(13.5%) had multiple lesions. 3. The lower extremities(40 cases, 50.6%) were the most common sites followed by the upper extremities(16 cases, 20.9%), abdomen(5 cases, 6.3%), shoulders(4 cases, 5.0%), back(3 cases, 3.7%), hands(3 cases, 3.7%), neck(2 cases, 2.5%), buttock(2 cases, 2.5%), breast(1 cases, 1.2%), face(1 cases, 1.2%), axilla(1 cases, 1.2%) and foot(1 cases, 1.2%). 4. The most common color was brown (50 cases, 74.7%), and other common colors were red(10 cases, 14.9%), black (4 cases, 5.9%), violaceous (3 cases, 4.5%). 5. Histopathologically, fibrous types were seen in 51cases(76.1%), cellular types in 6 cases (8.9%), mixed types in 7 cases(10.4%), and sclerosing hemangiomas in 3 cases(4.6%). 6. All lesions were positive for factor XIIIa. However, the degree of staining was different according to the duration; less than 1 year(10 cases-strong positive), between 1 to 5 years (3 cases-strong positive, 7 cases-moderate positive), more than 5 years(2 cases-moderate positive, 3 cases-weak positive). 7. Results of Masson's trichrome and Verhoeff-van Gieson stain showed that normal appearanced collagen and elastic fibers were increased in the lesion according to the duration and mucin depositions were also more increased in the old lesion than the younger lesion by alcian blue stain. Conclusion:On the basis of our result, we believe that factor XIIIa-positive cells seen in dermatofibroma represent reactive stromal cells rather than true tumor cells. Further studies are needed to determine whether the other factors are related to the pathogenesis of dermatofibroma. (Korean J Dermatol 2000;38(4):500~505)

피부 형질세포종을 동반한 다발성 골수종

안성구(Sung Ku Ahn),이승헌(Seung Hun Lee),신동환(Dong Hwan Shin) 대한피부과학회 1987 대한피부과학회지 Vol.25 No.6

A 52-year-old man with multiple myeloma developed cutaneous nodules while being treated with melphalan and prednisolone. A biopsy specimen showed dermal infiltration by well differentiated plasma cells similar to those found on bone marrow biopsy. The use of peroxidase anti-peroxidase to demonstrate the monoclonality or polyclonality of the cytomplasmic immunoglohulins in the tumor cells revealed a positivity for IgG and 1 chain (ie, monotypic staining). Ultrastructurally, each plasmacytoma cell contained varyting amounts of rough endoplasmic reticulum and Golgi-apparatus. The cutnneous nodules completely disappeared after radiotherapy

피부 평활근종의 임상 및 병리조직학적 연구

안성구(Sung Ku Ahn),함익병(Ki Byeong Haam),한승경(Seung Kyung Hann),이승헌(Seung Hun Lee),강원형(Won Hyoung Kang),이성낙(Sung Nack Lee) 대한피부과학회 1991 대한피부과학회지 Vol.29 No.2

We reviewed 50 skin biopsies obtained from 40 patients with cutaneous leiomyoma during a 10 year period from January 1980 to December 1989. The result.s were summarized as follows: 1) Sex distribution showed a female preponderance with male.female ratio of 1: 1.9. 2) The age distribution was relatively even from childhood to old age. The mean age was 47,8 years, 3) The most common site of lesion was lower leg(34%) followed by hand(24%), foot(12% ), anterior chest(8% ), face(6% ), neck(4%), upper arm(4% ), labia major(2 %) and nipple(2%). 4) The lesions were relatively well circumscribed subcutaneous nodule, papule, plaque and nodule which measured between 0.3cm to 5cm in size and their colors were erythematous to light brown. 5) The most common clinical and histopathologic type was angioleiomyoma(75%) followed by solitary piloleiomyoma(12.5%), multiple piloleiomyoma(7,5%) and solitary genital leiomyoma(5% ). Angioleiomyomas were clincally associated with cavernous hemangioma, myoma of uterus and systemic lupus erythematosus. 7) The clinical diagnoses were mass(30% ), epidermal cyst(20%), dermatofibroma (12.5% ), leiomyoma(12,5%), neurilemmoma(5%), lipoma(5%), calcifying epithelioma(2.5%), metastatic carcinoma(2.5%), morphea(2.5% ), fibrocystic disease(2.5%), glomus tumor(2.5 % ) and Dupuytrens contracuture(2..5%).(Kor J Dermstol 29(2):181 186)

동면 선종

안성구(Sung Ku Ahn),이승현(Seung Hun Lee),이성낙(Sung Nack Lee) 대한피부과학회 1991 대한피부과학회지 Vol.29 No.1

We Report a hibernoma which ocurred in a 30-year-old male, who had 2cm sized, well-marginated, subcutaneous mass on the right side of the scapula. Histopathologic finding showed the tumor was composed of uniform granular or multivacuolated, eosinophilic, round to ovoid cells with small and peripherally plnced nuclei. Total exrision of the tumor was performed. No recurrence was observed for 5 months after surgical excision. (Kor J Dermatol 29(1):100 103, 1991)

피부로 전이된 방광암

안성구(Sung Ku Ahn),원진형(Jin Hyong Won),곽호(Ho Kwack),이승헌(Seung Hun Lee),이성낙(Sung Nack Lee) 대한피부과학회 1991 대한피부과학회지 Vol.29 No.2

A 70 year-old male visited with 1 month history of dark brown, hard, non-movable subcutaneous nodule on the anterior chest. Histopathologic findings were compatible with the metastatic transitional cell carcinoma of the urinary bladder. (Kor J Dermatol 29(2):271--274)