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      • 작잠(작蠶)에 관(關)한 연구(硏究)

        박병희 ( B. H. Park ),송기언 ( K. E. Song ),이상풍 ( S. P. Lee ),박광의 ( K. E. Park ) 한국잠사학회 1965 한국잠사곤충학회지 Vol.5 No.-

        本 實驗은 中性子 및 γ-Ray를 작蠶 踊體 및 蠶卵에 照射하여 突然變異體를 얻고저 行하였든 바 그 結果는 다음과 같다. 1) 踊體에 對한 中性子 處理區는 모두 羽化 하였으나 交尾不能이였으며 4000γ 區만은 産卵은 하였지마는 全部 不受精卵이였고 卵內容物도 없었으며 一部는 卵殼에 구멍이 뚫려있었다. 2) 秋期 二化期에 卵에 對한 中性子處理區에서는 50~60%의 乳化率을 보였으나 卵에 對한 γ-Ray 處理區는 全然 孵化하지 못하였다. 3) 中性子에 對한 踊體의 減受性은 卵보다 弱하다. 4) 孵化率은 γ-線量에 反比例한다. 5) γ-4000區에 繭質이 多少 優秀한 突然變異體를 얻었는데 來年度에 次代檢定을 行할 豫定이다. I. Breeding of tussah silkworm(preliminary report). The preliminary examination for bleeding has been carried out since 1963 in tussah silkworms. 1) The strain(l-MG-B) of the heaviest silk quantity was the green silkworm and brown cocoon in univoltine, and the strains(2-G-B, 2-MG-B) of the heaviest silk quantity were also the green silkwom and brown cocoon in bivoltine in both spring and fall in 1965. 2) It looks like the voltinism, the body color and the cocoon color have reached to pure line up to 1965. II. Best place for the winter of tussah pupa. This work was aimed to find out good ways for the winter of tussah pupa. 1) The hatch of bivoltine was better than that of univoltine. 2) The cocoons covered with the leaves were good in the emergence of moth. 3) The cocoons which were kept at natural temperature till the first emergence of moths would show bad in both hatch and emergence. 4) If some of the pupae kept under natural condition were controled at proper temperature for a few days, hatch and laying eggs were best. 5) The best places for the winter were the egg storage and the rearing room. III. Relation between incubation temperature and voltinism. 1) When the tussah pupa are kept at natural temperature during winter, the moths do not come out of the pupa. 2) There is no difference between about 18℃ and about 25℃ during incubation in hatching ratio. 3) The tussah silkworms of univoltine in mortality are stronger than that of bivoltine. 4) There is not any relation between voltinism and high or low temperature for pupa and eggs. IV. Induced mutation by gamma-ray and neutron in tussah silkworm. This work was carried out in order to induce the mutation by treating the pupa or the eggs of tussah silkworm with gamma my and neutron. The results obtained are as follows. 1. Though the whole pupa treated with neutron become moths, the moths have no ability to copulate each other. The only moths emerged from pupa treated with neutron, 4000γ are able to lay all un-fertilized eggs, some of which have a hole on the surface and nothing of contents. 2. The non-diapause eggs are treated with neutron in spring, but the hatching ratio is 50∼60 percent, but the whole eggs treated with gamma ray are never hatched. 3. The sensitivity of the pupa to neutron is weaker than that of the eggs. 4. The hatching ratio is in direct proportion to the gamma ray dose. 5. Author find out a new mutant which is excellent in the cocoon quality, so he will do the progeny test next hear.

      • 견(絹) Fibroin의 효소분해(酵素分解)에 관(關)한 연구(硏究) (3) -가잠(家蠶)의 원종(原種) 및 교잡종견사(交雜種繭絲)의 Fibroin 분해율(分解率) 차이(差異)-

        이용우 ( Y. W. Lee ),최병희 ( B. H. Choe ),송기언 ( K. E. Song ),남중희 ( J. H. Nahm ) 한국잠사학회 1976 한국잠사곤충학회지 Vol.18 No.2

        우리나라 장려잠품종의 원종과 교잡종견의 fibroin 분해율 및 각종 특수품종견에 대한 fibroin 분해율과 견사형태와의 관계를 구명코저 시험하여 다음 결과를 얻었다. 1. 원종과 교잡종독의 fbroin 효소분해에 있어서 원종은 F1 교잡종에 비하여 분해율이 높았다. 2. 양 원종의 평균 fbroin 분해율은 그 교잡종의 분해율과 정상관(r=+0.86)이 있었고 그 회귀방정식은 y=0.81x+0.04이다. 3. 교잡종견사 fibroin의 분해율에 있어서 잠103×잠104가 다른 장훈품종에 비하여 분해율이 낮았다. 4. 각종 특수품종 견사의 fibroin 분해에 있어서 품종간에 분해율차이가 인정되었고 견사섬도와 fibroin 분해율과는 낮은 정상관(r=0.6)이 있었다. 5. 이상의 결과에서 견 fibroin 분해율은 유전적 성질을 가지므로 잠품종육종에 있어서 이와 관련된 실용형질 선발에 marker로서 이용될 수 있을 것으로 사료된다. The study was carried out to investigate the variations in the fibroin hydrolysis of leading silkworm varieties in Korea, and the relationship between the fibroin hydrolysis of the silkworm varieties with some characteristics and silk morphological property. The results obtained are summarized as follows: 1) The fibroin hydrolyzing ratio of both parent silkworm cocoons was reduced in the F1 hybrids. 2) A positive corelation (r=+0.86) was approved between the mid-parent value and the F1 hybrid in the fibroin hydrolysis. 3) It was disclosed that the fibroin hydrolyzing ratio of Jam 103×Jam 104 was the lowest among the leading silkworm F1 hybrids. 4) The fibroin hydrolysis was affected by the silk morphological property, showing a positive corelation between the size of cocoon filament and the fibroin hydrolyzing ratio. 5) It was thought that the determination of the fibroin hydrolyzing ratio was useful as a marker for the selection of its related economical characters in silkworm breeding, because the fibroin hydrolysis of parents silkworm cocoons was heritable in their F1 hybrids.

      • 견(絹) Fibroin의 효소분해(酵素分解)에 관(關)한 연구(硏究) (2) -견층부위별(繭層部位別) Fibroin 분해율(分解率) 차이(差異)와 견사물성과(繭絲物性)과의 관계(關係)-

        이용우 ( Y. W. Lee ),최병희 ( B. H. Choe ),송기언 ( K. E. Song ),남중희 ( J. H. Nahm ) 한국잠사학회 1976 한국잠사곤충학회지 Vol.18 No.1

        각종 고치의 견층부위별 fibroin 효소분해율의 차이 및 견사물성과의 관계를 구명코자 시험하여 다음과 같은 결과를 얻었다. 1. 각종 고치의 견층부위별 fibroin 효소분해율은 외층에서 내층으로 갈수록 높았다. 2. 이상용견의 견층부위별 fibroin 분해율은 정상견에 비하여 높았으며 옥견>박피견>사용견>정견의 순위를 보이었다. 3. 원종인 잠111과 잠112의 fibroin 분해율 및 sericin 함유량은 교유종인 잠 111×잠112보다 높았다. 4. 인공사료육잠견의 견층부위별 fibroin의 분해율은 외층보다 내층으로 갈수록 증가되었으며 sericin 함유량은 외층에서 내층으로 갈수록 감소되었으나 최내층에서 약간 증가되는 경향을 보이었다. 5. 견층부위별 연편사의 절단강력은 외층에서 내층으로 갈수록 감소되는 경향이었고 이상용견의 절단강역은 정견에 비하여 저하되었다. 6. 견층부위별 fibroin 분해율과 견사의 절단강력과는 부의 상관(r=-0.8)이 인정되고 그 회귀방정식은 y=-0.29x+5.07이었다. The study was carried out to investigate the variations in the fibroin hydrolysis of different cocoon shell layers, and the relationship between the hydrolysis and silk physical property. The obtained results are as follows: 1. The fibroin hydrolyzing ratio was highest at the inner layer of cocoon shell and, next in order, at the middle layer and at the outer layer. 2. The fibroin hydrolyzing ratio of abnormal cocoons was higher than normal cocoon and it was different among them, tile highest was double cocoon, thin shell cocoon and perforated cocoon in order. 3. The sericin content and fibroin hydrolyzing ratio of Jam 111 and Jam 112 was higher than that of Jam 111×Jam 112. 4. The fibroin hydroiyzing ratio of the cocoons fed with the artificial diets was increased at the inner layer. The sericin content of those lessened at the inner layer, however, it slightly increased at the most inner layer more than at the inner layer. 5. The breaking strength of the degummed silk fiber of different cocoon layers was reduced at the inner layer. The breaking strength of abnormal silk fiber was less than that of normal silk fiber. 6. A negative correlation (r=-0.8) was approved between fibroin hydrolyzing ratio and breaking strength of silk fiber, and the regression line was Y=-0.29x+5.07.

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